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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Immunol. | doi: 10.3389/fimmu.2019.01890

NK cell contributes to the immune risk profile in kidney transplant candidates .

  • 1University of Rochester, United States
  • 2Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, United States
  • 3Transplant Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, United States
  • 4Eurofins Viracor, United States
  • 5Division of Infectious Diseases, Center for Virology and Vaccine Research, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, United States

Background:
A previously proposed immune risk profile (IRP), based on T cell phenotype and CMV serotype, is associated with mortality in the elderly and increased infections post-kidney transplant. To evaluate if NK cells contribute to the IRP and if the IRP can be predicted by a clinical T cell functional assays, we conducted a cross sectional study in renal transplant candidates to determine the incidence of IRP and its association with sepcificNK cell characteristics and ImmuKnow® value.
Material and Methods:
65 subjects were enrolled in 5 cohorts designated by age and dialysis status. We determined T and NK cell phenotypes by flow cytometry and analyzed multiple factors contributing to IRP.
Results:
We identified 14 IRP+ (CMV seropositivity and CD4/CD8 ratio < 1 or being in the highest quintile of CD8+ senescent (CD28-/CD57+) T cells) individuals equally divided amongst the cohorts. Multivariable linear regression revealed a distinct IRP+ group. Age and dialysis status did not predict immune senescence in kidney transplant candidates. NK cell features alone could discriminate IRP- and IRP+ patients, suggesting that NK cells significantly contribute to the overall immune status in kidney transplant candidates and that a combined T and NK cell phenotyping can provide a more detailed IRP definition.
ImmuKnow® value was negatively correlated to age and significantly lower in IRP+ patients and predicts IRP when used alone or in combination with NK cell features.
Conclusion:
NK cells contribute to overall immune senescence in kidney transplant candidates.

Keywords: Immune senescence, NK cells, T cells, Pre kidney transplant, Immune risk profile

Received: 08 Mar 2019; Accepted: 26 Jul 2019.

Copyright: © 2019 DeWolfe, Aid, McGann, Ghofrani, Geiger, Helzer, Malik, Kleiboeker, Jost and Tan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: MD. Chen S. Tan, Beth Israel Deaconess Medical Center, Harvard Medical School, Division of Infectious Diseases, Center for Virology and Vaccine Research, Department of Medicine, Boston, United States, ctan@bidmc.harvard.edu