Frontiers reaches 6.4 on Journal Impact Factors

Original Research ARTICLE

Front. Mar. Sci., 22 July 2014 | https://doi.org/10.3389/fmars.2014.00024

Anti-inflammatory activity in selected Antarctic benthic organisms

Juan Moles1*, Anna Torrent2, M. José Alcaraz3, Ramon Ruhí2 and Conxita Avila1
  • 1Department of Animal Biology (Invertebrates), Biodiversity Research Institute (IrBIO), Universitat de Barcelona, Barcelona, Catalonia, Spain
  • 2R&D Department, Bioibérica, Barcelona, Catalonia, Spain
  • 3Department of Pharmacology and Pharmacodynamics, University of Valencia, Valencia, Spain

Antarctic benthos was prospected in search for anti-inflammatory activity in polar benthic invertebrates, in two different geographical areas: deep-bottoms of the Eastern Weddell Sea and shallow-waters of the South Shetland Islands. A total of 36 benthic algae and invertebrate species were selected to perform solubility tests in order to obtain extracts that were soluble at an innocuous ethanol concentration (0.2%) for cell culture, and further test them for anti-inflammatory activity. From these, ethanol extracts of ten species from five different phyla resulted suitable to be studied in cell macrophage cultures (RAW 264.7). Cytotoxicity (MTT method) and production of inflammatory mediators (prostaglandin E2, leukotriene B4, interleukin-1β) were determined at three extract concentrations (50, 125, 250 μg/mL). Bioassays resulted in four different species showing anti-inflammatory activity corresponding to three sponges: Mycale (Oxymycale) acerata, Isodictya erinacea, and I. toxophila; and one hemichordate: Cephalodiscus sp. These results show that Antarctic sessile invertebrates may have great value as a source of lead compounds with potential pharmaceutical applications.

Introduction

The Ocean harbors a rich source of both biological and chemical diversity. Although this diversity is the source of unique chemical compounds, its potential for pharmaceutical applications remains still underexplored (Kijjoa and Sawangwong, 2004; Albericio et al., 2010). Some marine natural products show useful pharmacological activities and are being developed either as analgesics or to treat inflammation (Jha and Zi-rong, 2004; Mayer et al., 2011). Many sessile and soft bodied benthic organisms possess defensive mechanisms based on the use of chemical compounds, which often display high biological activity (Faulkner, 1995). Recent studies on marine organisms are in fact providing many bioactive natural products, in larger percentages than terrestrial organisms (König et al., 2006; Newman and Cragg, 2012). In particular, organisms from temperate and tropical areas have been the most studied so far, while polar organisms remain almost unknown (Lebar et al., 2007; Avila et al., 2008; Blunt et al., 2013). During several cruises in the Southern Ocean we collected samples of benthic sessile and sluggish organisms in order to evaluate their potential for pharmacological applications. Thus, the aim of this study was to determine the potential anti-inflammatory and pain-killer applications of some Antarctic samples collected in the Eastern Weddell Sea and the South Shetland Islands. In order to do this, we tested the possible inhibition of the production of some important mediators of inflammation and pain. We tested ethanol (EtOH) extracts at different concentrations as a source of natural products from marine organisms. Since inflammation is caused by the release of chemicals from tissues and migrating cells (Korbut and Guzik, 2011), we decided to test the release of some of the most strongly implicated compounds in the inflammation reaction: the prostaglandins (PGs), leukotrienes (LTs), and interleukin-1 (IL-1).

Materials and Methods

Sample Collection

Marine benthic invertebrate samples were collected in the Eastern Weddell Sea (Antarctica) during the ANT XXI/2 cruise (November 2003–January 2004) on board the R/V “Polarstern” (AWI, Bremerhaven, Germany). Our samples were obtained from 7 stations at depths ranging from 230 to 600 m sampled with Agassiz and bottom trawls. During ACTIQUIM-I campaigns (2007–2008 and 2008–2009), algae and invertebrate samples from Deception, Livingston, and Snow Islands were sampled by SCUBA at 7 stations (ca. 0–15 m depth) (Table 1). Organisms were photographed alive and immediately frozen (−20°C) for chemical investigations. Organisms were selected according to potential interest based on ecological observations and/or previous ecological assays (Taboada et al., 2012). Voucher specimens or a portion of each sample was fixed in 10% formalin or 70% EtOH for taxonomical identification. These vouchers are deposited at the Dept. of Animal Biology (Invertebrates), University of Barcelona, Spain.

TABLE 1
www.frontiersin.org

Table 1. Data of the benthic algae and invertebrates used in this study, collected in the Weddell Sea and the vicinities of the South Shetland Islands (Deception, Livingston, and Snow Islands) during ANT XXI/2, ACTIQUIM-1, and ACTIQUIM-2 cruises.

Chemical Extraction and Solubility Screening

When necessary, several conspecific specimens from the same station were extracted together in order to obtain enough extract for the experiments. Frozen selected samples were weighted and immediately lyophilized for 24–48 h. Subsequently, dehydrated samples were re-weighted, pounded, and exhaustively extracted with absolute ethanol (1:10 w/v) helping with ultrasonic baths of 10 min. After filtering, the ethanol of the remaining extracts was evaporated in vacuo. The solid ethanol extracts were sequentially solubilized at different EtOH/H2O concentrations: 125 μg/μL EtOH 100%, 5 μg/μL EtOH 4%, and 0.25 μg/μL EtOH 0.2% and freeze-dried immediately. Cell culture was performed only with ethanol extracts that remained soluble at EtOH 0.2%, as it is the highest ethanol concentration innocuous to cells. Ten species with soluble extracts at 0.2% EtOH were chosen to perform anti-inflammatory assays according to the solubility tests. We similarly extracted more individuals and/or the rest of the frozen samples selected in order to obtain enough extract for the anti-inflammatory assays. Infrared spectra of samples and replicates were performed for all the species in order to discard contamination due to extraction methods of the ten species. Wet, dry, and extract's weight of the species selected and those rejected are shown in Supplementary Tables S1, S2, respectively.

Cell Culture

The mouse macrophage RAW 264.7 cell line (American Type Culture Collection, Manassas, VA, U.S.A.) was cultured in DMEM medium supplemented with 5% fetal bovine serum. Cells were incubated with marine organisms' extracts at concentrations of 50, 125, and 250 μg/mL (dissolved in 0.2% ethanol). The cells were stimulated with lipopolysaccharide (LPS; 1 μg/ml) and incubated for 24 h, in the presence or absence of extracts. Dexamethasone (1 μM) was used as a reference anti-inflammatory drug. Each test was performed in quadruplicate. Toxicity of the extracts at the concentrations tested was assessed by the mitochondrial-dependent reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide to colored formazan (MTT method). The production of inflammatory mediators was determined in cell supernatants by measuring: prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) by radioimmunoassay and interleukin-1β (IL-1β) by ELISA. Statistical significance was established by ANOVA followed by Dunnett's test. Results indicate mean ± SE (at least n = 8 for each group). *P < 0.05, **P < 0.01 with respect to LPS control.

Results

Solubility Tests

A total of 36 ethanol extracts from benthic algae and invertebrates were dissolved at decreasing ethanol concentrations, down to 0.2% EtOH, to perform the bioactivity assays at an innocuous ethanol concentration (Table 1). Ethanol extracts of ten species from five different phyla: Achrophyta (2), Porifera (4), Chordata (2), Echinodermata (1), and Hemichordata (1), resulted suitable, i.e., soluble at EtOH 0.2%, to cell culture (asterisks in Table 1).

Cytotoxicity

EtOH extracts of the alga Desmarestia menziesii, the sponge Mycale (Oxymycale) acerata, and the seastar Odontaster validus exhibit cytotoxicity at the two higher concentrations (125 and 250 μg/mL; Figure 1). Certain cytotoxicity, only at the highest concentration, was found in the alga Desmarestia anceps, the sponge Isodictya toxophila, and the hemichordate Cephalodiscus sp. Finally, the sponges Isodictya erinacea and Homaxinella cf. balfourensis, the ascidiacean Synoicum adareanum, and the thaliacean Salpa cf. thompsoni were not cytotoxic at the concentrations tested.

FIGURE 1
www.frontiersin.org

Figure 1. Toxicity and anti-inflammatory effects of the extracts of Isodictya erinacea, I. toxophila, Mycale (Oxymycale) acerata, and Cephalodiscus sp. in the mouse macrophage cell line RAW 264.7. Cytotoxicity (MTT method) and amount of inflammatory mediators released: PGE2, LTB4, and IL-1β, for each concentration tested (250, 125, 50 μg/mL), LPS control, and the reference anti-inflammatory drug dexamethasone (DX). B indicates the non-stimulated cell control. Statistical significance (ANOVA followed by Dunnett's test): *P < 0.05, **P < 0.01, with respect to LPS control.

Release of Inflammatory Mediators

Desmarestia menziesii extracts decreased the release of IL-1β and PGE2 at cytotoxic concentrations (Supplementary Figure S1). Similarly, the extract of Odontaster validus reduced the release of IL-1β at the highest concentration, which had cytotoxic effects. The EtOH extract of the brown alga D. anceps inhibited PGE2 release at non-cytotoxic concentrations (50 and 125 μg/mL), while showing no action on the other mediators. The extract of the salpid Salpa cf. thompsoni reduced significantly and dose-dependently the release of IL-1β and PGE2. However, at the highest concentration (250 μg/mL) increased the release of LTB4. The EtOH extract of the demosponge Homaxinella cf.balfourensis reduced significantly the release of IL-1β and PGE2 only at the highest, but not cytotoxic, concentration (250 μg/mL). Finally, the extract of the colonial ascidiacean, Synoicum adareanum, increased the release of PGE2 and LTB4 at the highest concentration, having no effect at lower concentrations (Supplementary Figure S1).

The extract of Isodictya erinacea inhibited significantly and dose-dependently the release of IL-1β and PGE2, although not affecting LTB4 (Figure 1). The extracts of both the sponge Isodictya toxophila and the hemichordate Cephalodiscus sp. decreased the release of IL-1β and LTB4 at two non-cytotoxic concentrations (50 and 125 μg/mL) and also diminished PGE2 release at the intermediate concentration (125 μg/mL) (Figure 1). Mycale (Oxymycale) acerata's extract decreased the release of IL-1β, PGE2, and LTB4 at the lower concentration of 50 μg/mL, which was not cytotoxic (Figure 1).

Discussion

Cytotoxicity was determined in order to evaluate the possible further applications as anti-inflammatory activity. Among the three species which exhibit more cytotoxic activity two were previously investigated. The brown macroalgae Desmarestia menziesii is known to produce plastoquinones, which have been suggested to present cytotoxic activity against leukemia cells, toxicity to fish, and inhibit mitosis of fertilized sea urchin eggs (Rivera, 1996). Also Mycale (Oxymycale) acerata is a chemically bioactive species displaying cytotoxicity to sea urchin gametes (McClintock et al., 1990). However, the sea star Odontaster validus is here reported as a cytotoxic species for the first time.

The species with intermediate cytotoxicity, i.e., only at the highest concentration, have been reported previously with similar cytotoxic activity. Desmarestia anceps possess antibacterial and diatom's antifouling activity (Laturnus et al., 1996; Huang et al., 2006). Cytotoxicity against cells of human colon adenocarcinoma was recently proved in the deep-sea sponge Isodictya toxophila (Turk et al., 2013). Several studies in equatorial waters showed that isolates from Cephalodiscus gilchristi, the cephalostatins, exhibit potent cytotoxicity toward murine P388 lymphocytic leukemia cell line (Rudy et al., 2008). In fact, cephalostatin 1 proved to be one of the most powerful cancer cell growth inhibitors (Moser, 2008). However, no Cephalodiscus species from the Southern Ocean had been tested for cytotoxic properties before our study and there are no molecules isolated so far (Avila et al., 2008).

Four species were not cytotoxic at the concentrations tested. The sponges Isodictya erinacea and Homaxinella cf. balfourensis have been previously tested and no cytotoxic activity was found (McClintock et al., 1990; Turk et al., 2013). Similarly, to the polyketide isolated from Synoicum adareanum, palmerolide A, which presented no cytotoxicity against several cell lines, in our study the macrophages RAW 264.7 were not affected by the different concentrations of the extracts tested (Diyabalanage et al., 2006). Likewise, several polyunsaturated acids with hemolytic activity have been isolated from individuals of Salpa thompsoni (Mimura et al., 1986).

The species providing the best results for avoiding the release of inflammatory mediators at non-cytotoxic concentrations were the hemichordate Cephalodiscus sp., and the sponges Isodictya erinacea, I. toxophila, and Mycale (Oxymycale) acerata. Since inflammation is caused by the release of chemicals from tissues and migrating cells, as mentioned above, we may conclude that the active extracts could be useful in avoiding inflammation and pain. Thus, these extracts possess promising molecules with anti-inflammatory activity, potentially useful in pharmacology. Finally, our study shows that Antarctic benthic invertebrates may have a great value as a source of lead compounds with potential pharmaceutical applications, such as painkillers. For this reason, it will be very interesting to isolate and to test the isolated molecules responsible of the release-avoidance of inflammatory mediators, which might provide future anti-inflammatory drugs.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

The authors wish to thank J. Vázquez, J. Cristobo, L. Núñez-Pons, and S. Taboada for field and laboratory support. Thanks are due to Prof. W. Arntz and the crew of the R/V of Polarstern for allowing our participation in the Antarctic cruise ANT XXI/2 (AWI, Bremerhaven, Germany). Funding was provided by the Spanish government through the ACTIQUIM Projects (CGL2004-03356/ANT, CGL 2007-65453/ANT, and CTM2010-17415/ANT) and by the pharmaceutical company Bioibérica.

Supplementary Material

The Supplementary Material for this article can be found online at: http://www.frontiersin.org/journal/10.3389/fmars.2014.00024/abstract

References

Albericio, F., Álvarez, M., Cuevas, C., Francesch, A., Pla, D., and Tulla-Puche, J. (2010). “The sea as a source of new drugs Fernando,” in Molecular Imaging for Integrated Medical Therapy and Drug Development, eds N. Tamaki and Y. Kuge (Tokyo: Springer Japan), 237–249.

Avila, C., Taboada, S., and Núñez-Pons, L. (2008). Antarctic marine chemical ecology: what is next? Mar. Ecol. 29, 1–71. doi: 10.1111/j.1439-0485.2007.00215.x

CrossRef Full Text

Blunt, J. W., Copp, B. R., Keyzers, R. A., Munro, M. H. G., and Prinsep, M. R. (2013). Marine natural products. Nat. Prod. Rep. 30, 237–323. doi: 10.1039/c2np20112g

CrossRef Full Text

Diyabalanage, T., Amsler, C. D., McClintock, J. B., and Baker, B. J. (2006). Palmerolide A, a cytotoxic macrolide from the antarctic tunicate Synoicum adareanum. J. Am. Chem. Soc. 128, 5630–5631. doi: 10.1021/ja0588508

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Faulkner, D. J. (1995). Chemical riches from the ocean. Chem. Br. 31, 680–684.

Huang, Y. M., McClintock, J. B., Amsler, C. D., Peters, K. J., and Baker, B. J. (2006). Feeding rates of common Antarctic gammarid amphipods on ecologically important sympatric macroalgae. J. Exp. Mar. Biol. Ecol. 329, 55–65. doi: 10.1016/j.jembe.2005.08.013

CrossRef Full Text

Jha, R. K., and Zi-rong, X. (2004). Biomedical compounds from marine organisms. Mar. Drugs 2, 123–146. doi: 10.3390/md203123

CrossRef Full Text

Kijjoa, A., and Sawangwong, P. (2004). Drugs and cosmetics from the sea. Mar. Drugs 2, 73–82. doi: 10.3390/md202073

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

König, G. M., Kehraus, S., Seibert, S. F., Abdel-Lateff, A., and Müller, D. (2006). Natural products from marine organisms and their associated microbes. Chembiochem 7, 229–238. doi: 10.1002/cbic.200500087

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Korbut, R., and Guzik, T. J. (2011). “Inflammatory Mediators and Intracellular Signalling,” in Principles of Immunopharmacology, 3rd Edn., eds F. P. Nijkamp and M. J. Parnham (Basel: Birkhäuser). doi: 10.1007/978-3-0346-0136-8_7

CrossRef Full Text

Laturnus, F., Wiencke, C., and Klöser, H. (1996). Antarctic macroalgae - sources of volatile halogenated organic compounds. Mar. Environ. Res. 41, 169–181. doi: 10.1016/0141-1136(95)00017-8

CrossRef Full Text

Lebar, M. D., Heimbegner, J. L., and Baker, B. J. (2007). Cold-water marine natural products. Nat. Prod. Rep. 24, 774–797. doi: 10.1039/b516240h

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Mayer, A. M. S., Rodríguez, A. D., Berlinck, R. G. S., and Fusetani, N. (2011). Marine pharmacology in 2007-8: marine compounds with antibacterial, anticoagulant, antifungal, anti-inflammatory, antimalarial, antiprotozoal, antituberculosis, and antiviral activities; affecting the immune and nervous system, and other miscellaneous mec. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 153, 191–222. doi: 10.1016/j.cbpc.2010.08.008

CrossRef Full Text

McClintock, J. B., Heine, J., Slattery, M., and Weston, J. (1990). Chemical bioactivity in common shallow-water Antarctic marine invertebrates. Antarct. J. U. S. 25, 204–206.

Mimura, T., Okade, M., Satake, M., Nakanishi, T., Inada, A., Fujimoto, Y., et al. (1986). Fatty acids and sterols of the tunicate, Salpa thompsoni, from the Antarctic Ocean: chemical composition and hemolytic activity. Chem. Pharm. Bull. 34, 4562–4568. doi: 10.1248/cpb.34.4562

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Moser, B. R. (2008). Review of cytotoxic cephalostatins and ritterazines: isolation and synthesis. J. Nat. Prod. 71, 487–491. doi: 10.1021/np070536z

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Newman, D. J., and Cragg, G. M. (2012). Natural products as sources of new drugs over the 30 years from 1981 to 2010. J. Nat. Prod. 75, 311–335. doi: 10.1021/np200906s

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Rivera, P. (1996). Plastoquinones and a chromene isolated from the Antarctic brown alga Desmarestia menziesii. Bol. Soc. Chil. Quim. 41, 103–105.

Rudy, A., López-antón, N., Dirsch, V. M., and Vollmar, A. M. (2008). The cephalostatin way of apoptosis. J. Nat. Prod. 71, 482–486. doi: 10.1021/np070534e

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Taboada, S., Núñez-Pons, L., and Avila, C. (2012). Feeding repellence of Antarctic and sub-Antarctic benthic invertebrates against the omnivorous sea star Odontaster validus. Polar Biol. 36, 13–25. doi: 10.1007/s00300-012-1234-z

CrossRef Full Text

Turk, T., Ambrožič Avguštin, J., Batista, U., Strugar, G., Kosmina, R., Čivović, S., et al. (2013). Biological activities of ethanolic extracts from deep-sea Antarctic marine sponges. Mar. Drugs 11, 1126–1139. doi: 10.3390/md11041126

Pubmed Abstract | Pubmed Full Text | CrossRef Full Text

Keywords: inflammatory inhibitor, Antarctic benthic invertebrates, sponge, hemichordate, marine natural products

Citation: Moles J, Torrent A, Alcaraz MJ, Ruhí R, and Avila C (2014) Anti-inflammatory activity in selected Antarctic benthic organisms. Front. Mar. Sci. 1:24. doi: 10.3389/fmars.2014.00024

Received: 21 May 2014; Accepted: 30 June 2014;
Published online: 22 July 2014.

Edited by:

Donatella De Pascale, National Research Council- CNR, Italy

Reviewed by:

Maria Rosaria Coscia, National Research Council of Italy, Italy
Giovanna Romano, Stazione Zoologica Anton Dohrn, Italy

Copyright © 2014 Moles, Torrent, Alcaraz, Ruhí, and Avila. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Juan Moles, Department of Animal Biology (Invertebrates), Biodiversity Research Institute (IrBIO), Universitat de Barcelona, Av. Diagonal 643, 08028 Barcelona, Catalonia, Spain e-mail: moles.sanchez@gmail.com