Original Research ARTICLE
Transparent Exopolymeric Particles (TEP) Selectively Increase Biogenic Silica Dissolution From Fossil Diatoms as Compared to Fresh Diatoms
- Laboratoire des Sciences de l'Environnement Marin, UMR 6539 CNRS, Institut Universitaire Européen de la Mer (IUEM), Université de Bretagne Occidentale (UBO), Plouzané, France
Diatom production is mainly supported by the dissolution of biogenic silica (bSiO2) within the first 200 m of the water column. The upper oceanic layer is enriched in dissolved and/or colloidal organic matter, such as exopolymeric polysaccharides (EPS) and transparent exopolymeric particles (TEP) excreted by phytoplankton in large amounts, especially at the end of a bloom. In this study we explored for the first time the direct influence of TEP-enriched diatom excretions on bSiO2 dissolution. Twelve dissolution experiments on fresh and fossil diatom frustules were carried out on seawater containing different concentrations of TEP extracted from diatom cultures. Fresh diatom frustules were cleaned from the organic matter by low ash temperature, and fossil diatoms were made from diatomite powder. Results confirm that newly formed bSiO2 dissolved at a faster rate than fossil diatoms due to a lower aluminum (Al) content. Diatom excretions have no effect on the dissolution of the newly formed bSiO2 from Chaetoceros muelleri. Reversely, the diatomite specific dissolution rate constant and solubility of the bSiO2 were positively correlated to TEP concentrations, suggesting that diatom excretion may provide an alternative source of dSi when limitations arise.
The terrestrial lithosphere is composed of 27% (by weight) silicon. As a nutrient and required element of various marine organisms, silicon has an important part in biogeochemical processes. At a global scale, silica and carbon cycles are coupled by silicifying organisms via photosynthesis. In the marine realm, diatoms (Bacillariophyceae), unicellular phytoplankton with an absolute requirement for silicon to build their frustules composed of amorphous polymerised silica (bSiO2), are key silicifying organisms that play an important role in the marine biogeochemical cycling of carbon. They are responsible for nearly 40% of the global primary production (Nelson et al., 1995; Rousseaux and Gregg, 2013). This contribution to the carbon cycle varies greatly according to the oceanic region and period of the year. For example, the contribution of diatoms to the global primary production varies between 25% in oligotrophic conditions (Brzezinski et al., 2011) to 75% in eutrophic ocean areas (Nelson et al., 1995). Diatoms are also a major component of the marine biological carbon pump (Smetacek, 1999; Jin et al., 2006; Sanders et al., 2014) and are the base of some of the most successful food webs (Irigoien et al., 2002).
Approximately 50–60% of diatomaceous biogenic silica (bSiO2) produced in the euphotic layer is remineralized within the first 100–200 m of the water column (Tréguer et al., 1995; Van Cappellen et al., 2002; Passow et al., 2003). This process of bSiO2 dissolution provides diatoms with their main source of dissolved silicon, in the form of dissolved silica (dSi), which in turn, supports diatom growth for 50% of the global silicon budget established by Tréguer and De La Rocha (2013). Silica dissolution in the surface ocean of the northwest African upwelling regions was sufficient to supply all silicic acid taken up by phytoplankton (Nelson and Goering, 1977; Nelson et al., 1981). As such, biogenic silica dissolution is an important process that needs to be better understood and quantified, with particular emphasis on its variability in different environmental contexts. The dissolution of bSiO2 depends on abiotic factors, such as specific surface area, temperature, salinity, pressure, pH, and aluminum concentration (Van Cappellen et al., 2002; Loucaides et al., 2012). Less studied, biotic factors also influence bSiO2 dissolution. Diatom frustules are surrounded by an organic coating that needs to be removed by prokaryotes before dissolution of the frustule can begin (Bidle and Azam, 2001). In addition, biogenic silica dissolution depends on nutritive growth conditions of the diatoms (Boutorh et al., 2016). The process of silicification is known to be influenced by stressful conditions, such as nutrient limitation (Boyle, 1998; Takeda, 1998; Lasbleiz et al., 2014) and/or the presence of grazers (Pondaven et al., 2007).
Polysaccharides (EPS) are abundant in the surface waters of the global ocean and are typically found in the nutrient limited conditions at the end of a phytoplankton bloom (Passow, 2002a; Claquin et al., 2008). EPS are mainly secreted by diatoms and bacteria, though other types of phytoplankton can also contribute (Passow, 2002a). EPS are sticky and easily aggregate to form transparent exopolymeric particles (TEP), an essential trigger for mass flocculation during a phytoplankton bloom (Mari and Kiørboe, 1996; Passow, 2002b). Their size ranges from colloidal (1 kDa) to 100 μm, and they can reach abundances of 40,000 particles mL−1 or 11 mg Xeq L−1 (Mari and Burd, 1998; Passow, 2002b), contributing to ~10–25% of the DOM pool in surface waters (Passow, 2002b; Thornton, 2014). In such conditions, when turbulence favors collisions, TEP promote aggregation with cells included in the TEP matrix. In diatom aggregates bSiO2 dissolution is slower than in the surrounding seawater (Moriceau et al., 2007b). The decrease of bSiO2 dissolution rates inside aggregates have, for the first time, been attributed to the longer viability of aggregated diatoms (Garvey et al., 2007) and to higher dSi concentrations in the pore water due to adsorption processes (Moriceau et al., 2007a, 2014). Another hypothesis is that the TEP matrix also protects the bSiO2 against dissolution. EPS inhibits the dissolution of Al-enriched lithogenic silica (lSiO2), such as feldspar, kaolinite, and quartz (Welch and Ullman, 1993, 1996; Ullman et al., 1996). Conversely, in some cases, bacterial byproducts such as organic acids promote lSiO2 dissolution (Welch and Ullman, 1993, 1996; Vandevivere et al., 1994) which is much more resistant to dissolution than bSiO2. If this is true, it can be of great importance for the dissolution occurring both in the surface layer and in sediments. Similar to what can be found in the surface layer, TEP are sometimes concentrated at the surface of sediments due to massive sedimentation of aggregates or from the direct activity of benthic organisms (Wotton, 2004). Despite their abundance in the surface waters and sediments, two important components of the water column for bSiO2 dissolution processes, no study has measured the influence of TEP on the dissolution of diatomaceous bSiO2. In this context, the goal of the present study is to understand the influence of TEP concentrations on the dissolution of freshly cleaned and fossil diatom bSiO2.
Materials and Methods
In order to understand how diatom excretion products influence bSiO2 dissolution, we conducted two sets of dissolution experiments under batch conditions. Each set of conditions consisted of amorphous silica dissolution experiments in seawater containing different concentrations of TEP-enriched diatom excretion products. The dissolution of two different types of silica: Fossil diatoms and fresh diatoms, were evaluated. For the first set of experiments we used diatomite (fossil diatoms), for the second set of experiments we used dead diatoms Chaetoceros muelleri cleaned from their organic coating.
bSiO2 from diatomite (fossil diatoms) exists as a commercial product, a fine powder that is a chalk-like, soft, friable, earthy, very-fine grained, and siliceous sedimentary rock, usually light in color. The measured Si:Al ratio (moles:moles) of the diatomite was 4.8. Fresh bSiO2 from diatoms came from a culture of C. muelleri (strain CCAP 1010-3) provided by Ifremer's Argenton experimental station (Argenton, France). Fresh algae cultures were grown using a Conway culture medium (Salinity = 35.2 PSU), at 20°C with 100 μmol m−2 s−1 light on a 24:0 h light:dark cycle. The final cell concentration was 3.9 × 106 cells mL−1 after we isolated diatom byproducts and bSiO2. Ten liters of this culture solution was centrifuged at 3,000 g for 10 min at 15°C (Thermo Scientific Heraeus® Multifuge® 3SR Plus). Following protocols commonly used for bSiO2 dissolution experiments, the pellets were rinsed three times with milliQ water to remove salts and residual dSi, centrifuged, and then frozen at −20°C prior lyophilisation during 72 h. Fresh diatom bSiO2 was cleaned from the external organic membrane by low ash combustion in a plasma oven (Gala intrumente® Plasma—ACE5; Dixit and Van Cappellen, 2003; Loucaides et al., 2008). Despite the 10 L of highly concentrated diatom culture, we were only able to collect 254 μmol of bSiO2.
In order to extract byproducts from diatoms, another 10 L of C. muelleri culture at the stationary phase was kept in the dark for several days at 20°C. Diatom by-products were isolated from the diatoms by filtration through a 1 μm polycarbonate filter (C. muelleri, single cell size range from 4 to 6 μm). The resulting seawater was strongly enriched in TEP (TEP > 0.4 μm: 4.1 ± 1 mg Xeq L−1) and contained no diatoms. Five solutions were prepared from different mixtures of the TEP-enriched medium and natural seawater from the Bay of Brest, pre-filtered using a 0.22 μm polycarbonate filter (Salinity = 33.5 PSU, [SiOH4] = 8 μmol L−1, [NO3] = 18.35 μmol L−1, [NO2] = 0.20 μmol L−1, [PO4] = 0.45 μmol L−1). We expected an increasing TEP concentration range from the mixing of the two mother solutions. TEP-enriched to seawater vol:vol ratios were 0 for D1, 0.25 for D2, 0.5 for D3, 0.75 for D4, and 1 for D5. The salinity change in our medium from D1 to D5 did not exceed 1.7 PSU.
Nine 500 mL polycarbonate batches were used in the diatomite experiment: D1, D2, D3, D4, and D5, with D1 and D5 in triplicate. Each batch contained 15 mg of diatomite. For the second set of experiments with freshly cleaned diatoms, due to the low amount of bSiO2 collected from the 10 L of C. muelleri culture, only three polycarbonate batches of 125 mL were prepared using three dilutions: D1, D2, and D5. The initial bSiO2 concentrations were ~500 μM in all batches.
All batches were incubated in the dark, at 16°C, during 30 days on a shaking table for continuous agitation, allowing for a better homogenization of the particles in the batch. Batch cultures were kept ajar during the 30 days, in order to preserve gases exchanges, which have been proven to limit CO2 surplus and pH changes (Suroy et al., 2014, 2015; Boutorh et al., 2016). The dSi concentrations were measured on a daily basis for 4 days, and every second day until the end of the experiment.
TEP Sampling and Measurement
The method of Passow and Alldredge (1995) was used for TEP measurements. Three to five replicates for each solution were sampled at the beginning of the experiment. Under low-pressure conditions, 15 ml was filtered onto 0.4 μm polycarbonate filters (according to Passow and Alldredge, 1995) and stained with 0.5 mL of a 0.02% aqueous solution of pre-filtered Alcian blue in 0.06% acetic acid (pH 2.5). Filters were kept at −20°C until analysis. Filters were soaked for 2 h in 6 mL of 80% H2SO4 under agitation. The absorption measured at 787 nm in a 1 cm cuvette was converted into grams of Gum Xanthan equivalent per liter (g Xeq L−1) using a calibration curve done for our working solution of Alcian blue.
Dissolved and Particulate Biogenic Silica Measurements
Samples were filtered through 0.4 μm polycarbonates Millipore filters. Filtrates were preserved at 4°C in 15 mL polycarbonate falcon tubes prior to analysis for dSi. Millipore filters were dried during 24 h at 55°C inside Petri box, and then kept at room temperature before digestion and analysis for bSiO2. Filters were digested in 8 mL of NaOH (0.2 M) during 4 h at 90°C under constant agitation. Digestion was stopped by cooling the solution and neutralized with 2 mL of chloride acid (1 M). All samples were then centrifuged at 3,000 g at 20°C during 5 min. Supernatants containing the dissolved silica (dSi), were analyzed by AutoAnalyzer.
Silicic Acid (dSi) was analyzed using the automated colorimetric method on a Technicon AutoAnalyzer according to the protocol established by Tréguer and Le Corre (1975) and optimized by Aminot and Kérouel (2007). Samples are guided by a pump in a continuous flux, through a circuit in which dissolved silica (dSi) reacts with ammonium molybdate, at low pH, to produce β-silicomolybdique acid. This acid is then reduced by methylamino-4-phenol sulfite and a sodium sulfite solution, resulting in a final product absorbing at 810 nm.
In the present study, bSiO2 dissolution was monitored by following the dSi concentration with time (Equation 1) as described as:
The specific dissolution rate constant (K, d−1) was determined using the slope at the origin of the plot of the vs. time (Equation 2), which corresponds to exponential dissolution phases (Kamatani and Riley, 1979; Greenwood et al., 2001; Truesdale et al., 2005; Roubeix et al., 2008).
Where dSi(t) is dSi concentration (μmol L−1) measured at time t (day), dSi(0) is initial dSi concentration (μmol L−1), and dSi(e) is the dSi concentration reached at the plateau (μmol L−1). For diatomite dissolution experiments, dSi(e) corresponds to the apparent solubility of the bSiO2, because the 500 μM bSiO2 initially added was never totally dissolved in the dissolution batch. For the bSiO2 dissolution experiments using the freshly cleaned diatoms, dSi(e) is equal to the initial bSiO2 concentration (μmol L−1).
Correlations between the kinetic parameters of bSiO2 (specific dissolution rate constant and apparent solubility of the bSiO2) and TEP concentrations are tested with non-parametric Spearman rank correlation, using R software with function: cor.test() method = “Spearman.”
TEP concentrations (>0.4 μm) increased from D1 to D5, with values from 2.4 ± 0.2 to 4.7 ± 0.2 mg Xeq L−1 (Table 1).
Table 1. Initial TEP concentrations for each experimental batch condition, calculated specific dissolution rate constant (K), and apparent solubility of the bSiO2 for fossil diatoms cleaned diatoms.
The dissolution of bSiO2 was clearly visible in the two sets of experiments from the progressive increase of the dSi concentrations with time (Figures 1, 2). This increase was steeper for freshly cleaned diatoms than for fossil diatoms. In the fresh bSiO2 dissolution experiments, a small plateau of dSi concentration was observed when all the bSiO2 initially added to the batch was dissolved. In the dissolution batch containing fossil diatoms, the dSi concentrations stabilized after 120 h when they reached a plateau (Figure 1) equivalent to the apparent solubility of the bSiO2. The apparent solubility of the bSiO2 also increased from D1 to D5, with values from 62 μM in D1, 87 μM in D2, 102 μM in D3, 127 μM in D4, and 163 μM in D5 (Table 1, Figure 1).
Figure 1. bSiO2 dissolution of diatomite expressed as the increase in dSi concentration (μmol L−1) with time (days) for each TEP concentration D1 to D5 (see Table 1 for corresponding TEP concentrations). Dashed lines represent the linear regression for the initial part of the graphs. Gray lines represent the mean apparent solubility of the bSiO2.
Figure 2. bSiO2 dissolution of freshly cleaned diatom frustules of C. muelleri expressed as the increase in dSi concentration (μmol L−1) with time (days) for three TEP concentrations (see Table 1). Dashed lines represent the linear regression for the initial part of the graphs. Gray lines represent the dSi concentrations at the plateau, in these cases the initial bSiO2 concentrations.
Diatomite specific dissolution rate constants varied from 0.16 to 0.35 d−1 from D1 to D5 (Table 1). The specific dissolution rate constants of the freshly cleaned diatoms (C. muelleri) varied from 0.18 to 0.31 d−1 (Table 1). Our constants are close to the average specific dissolution rate constants of 0.15 ± 0.15 d−1 given for cleaned frustules by Roubeix et al. (2008). For diatomite dissolution, a significant correlation was revealed between specific dissolution rate constants and >0.4μm TEP concentrations (Figure 3; Spearman correlation = 0.7*, p-value = 0.022, N = 9 experiments). Similarly, a significant correlation was found between apparent solubility of the bSiO2 and >0.4 μm TEP concentrations (Figure 3; Spearman correlation = 0.867**, p-value = 0.002, N = 9 experiments). By contrast, no correlation between specific dissolution rate constants and TEP concentrations was observed for the freshly cleaned diatoms (Figure 3).
Figure 3. Correlations between the dissolution kinetics measured during the 12 dissolution experiments and TEP concentrations. On the left panel, bSiO2 specific dissolution rate constants (d−1) are plotted vs. TEP concentrations (>0.4 μm TEP, mg Xeq mL−1). On the right panel, bSiO2 apparent solubility of the bSiO2 (μmol L−1) are plotted vs. TEP concentrations (mg Xeq mL−1). Open squares represent C. muelleri and solid squares represent diatomite.
Specific dissolution rate constant and apparent solubility of bSiO2 were correlated with TEP concentration. We were able to create a concentration gradient of TEP in the dissolution batches from the different dilutions between the TEP-enriched medium and the natural filtered seawater. TEP concentrations in D1 that contained only 0.22 μm filtered natural seawater were negligible (2.4 ± 0.2 mg Xeq L−1). TEP are sticky particles (Passow, 2002b; Mari et al., 2017) that aggregate easily in solution, especially under continuous agitation. The different solutions also contained < 0.2 μm TEP. With this fraction, TEP concentrations were on average twice as high (Table 1). Our measurement suggests that in the filtered seawater, small TEP particles (lower than 0.2 μm) have aggregated into TEP particles with diameters larger than 0.4 μm. As most of the studies are measuring TEP >0.4 μm, we decided to refer only to these measurements when talking about TEP concentrations in the following discussion. Finally, we obtained TEP concentrations ranging from 2 to 6 mg Xeq L−1 that reflect what can be seen in situ according to Passow and Alldredge (1995), who gave natural values in a range of 0.02–11 mg Xeq L−1.
Dissolved silica (dSi) concentrations increased with time following the pattern generally seen in similar dissolution experiments. We observed some variability in the dissolution pattern with dSi concentrations data higher or lower than expected from Equation (1). At such high TEP concentrations, as seen in situ with high concentrations of phytoplankton, the seawater viscosity is influenced, affecting the swimming behavior of the zooplankton (Seuront, 2006). High viscosity decreases the diffusion of the dSi (Eyring, 1936), which may explain the variability of dSi concentrations as compared to the normal pattern, despite the mixing of the batches.
The bSiO2 from cleaned frustules dissolved on average five times faster than bSiO2 still surrounded by an external membrane (0.03 vs. 0.15 d−1, Roubeix et al., 2008). In our study, we measured similar dissolution rates for the cleaned bSiO2 from fresh C. muelleri with an average specific dissolution rate constant of 0.22 d−1. bSiO2 dissolved at 0.3 d−1 in the two batches containing less TEP, while in the third dissolution experiment the rate constant was 0.18 d−1. One experiment is not sufficient to clearly demonstrate a potential influence of diatom excretion on bSiO2 dissolution. However, a similar trend was observed in the study by Roubeix et al. (2008), in which they suggested a possible “blockage effect,” due to the physical coating of the bSiO2 surface that may slow down access to the surface by water molecules.
At similar TEP concentrations, bSiO2 from freshly cleaned frustules dissolved slightly faster than bSiO2 from diatomite (0.3 vs. 0.18 d−1, Table 1). During its stay in the sediment, “reverse weathering” processes such as Al-Si substitutions progressively decreased the reactivity of the bSiO2 (Van Bennekom et al., 1991). A partial explanation for the difference in the dissolution rate constant measured between diatomite and fresh bSiO2 could be a different Al content: The Si:Al ratio of the diatomite used in this study was 4.8 compared to the 20–40 commonly found for fresh diatoms (Ragueneau et al., 2005). In our study, most of the amorphous silica from the diatomite stayed in its particulate phase, with the apparent solubility of the bSiO2 going from 62 to 165 μmol L−1. By comparison, the solubility measured by Loucaides et al. (2008) for diatomite was 1,150 μM at 25°C and a pH of 8. However, the diatomite used in his study contained a lot less Al, and Al incorporation inside diatom frustules can also change solubility (Van Bennekom et al., 1991). An important result of our study is the correlation between the apparent solubility of the bSiO2 in the fossil diatoms batch and the TEP concentrations (Spearman correlation = 0.867**, p-value = 0.002). It has been suggested that dSi may adsorb on the TEP (Moriceau et al., 2014), which would explain the high dSi concentrations measured inside aggregates despite sometimes very low dSi concentrations in the surrounding seawater (Brzezinski et al., 1997). Our correlation between TEP concentrations and solubility of the bSiO2 tends to strengthen this hypothesis.
In our experiment, the influence of diatom byproducts on bSiO2 dissolution was monitored using the TEP concentrations because (1) diatoms generally produced EPS and TEP in larger quantities (Myklestad and Haug, 1972; Myklestad, 1974, 1995; Myklestad et al., 1989) and (2) previous experiments already showed an influence of polysaccharides on lithogenic SiO2 dissolution (Welch and Vandevivere, 1994). The bSiO2 specific dissolution rate constants of fossil diatoms were also correlated to TEP concentrations. As it was impossible to extract TEP from the rest of the diatom byproducts, and because the culture was not axenic, we must also have created a gradient of other molecules and bacteria concentration in the other dissolution batch. Analyses of diatom excretions at the end of a phytoplankton bloom or at the stationary phase in the culture showed considerable amounts of amino acids, proteoglycans, and extracellular polysaccharides containing residues of rhamnose, fucose, and galactose, with the carbohydrate contribution sometimes reaching 80–90% of the total extracellular release (Myklestad and Haug, 1972; Myklestad, 1974, 1995; Myklestad et al., 1989). Previous studies evidenced that fresh microbial EPS extracted from bacteria inhibit or promote the dissolution of lSiO2 (Welch and Vandevivere, 1994; Ullman et al., 1996), and organic acids such as glucuronic acids favor lSiO2 dissolution (Welch and Ullman, 1993, 1996; Vandevivere et al., 1994). However, the variation of the bSiO2 specific dissolution rate constants with the concentration of diatom byproducts could also be due to compounds other than polysaccharides, or to bacteria. To our knowledge no study has directly measured the potential influence of other byproducts such as amino acids. Concerning bacteria, proteolytic activities have little effect on bSiO2 dissolution, except for the removal of organic matter associated to the frustules occurring in the first days of the dissolution (Bidle and Azam, 2001). In our study we used frustules cleaned from their organic coating and indeed the dissolution of the bSiO2 from freshly cleaned diatoms was not increased by the bacteria gradient possibly resulting from our protocol. Roubeix et al. (2008) also hypothesized that bacterial colonization increases bSiO2 dissolution by creating a microenvironment with high ectoproteolytic activity at the diatom surface or by releasing organic compounds that can facilitate diatom bSiO2 dissolution (Roubeix et al., 2008). Rather, in their experiment they observed (1) no influence of bacterial activity and (2) a decrease of the dissolution rates when using cleaned frustules. Dissolution of the fossil bSiO2 was influenced by the gradient of diatom excretion/associated bacteria but had no influence or, possibly, an opposite effect on the bSiO2 dissolution of freshly cleaned frustules. If true, this would mean that diatoms or associated bacteria from the C. muelleri cultures are able to selectively increase the dissolution of bSiO2 from fossil diatoms but not the bSiO2 dissolution from the freshly cleaned diatoms.
More recently, other works from Akagi's team showed that by measuring rare earth elements on diatom frustules the dissolution of lithogenic silica in surface water could be an unexpected source of dSi for diatom growth (Akagi et al., 2011, 2014; Akagi, 2013a,b). Our experiment nicely complements their findings by adding a “how.” Indeed, as it has been shown in a forest where mycorrhizal symbiosis with the roots of trees promotes the weathering of nutrients by releasing organic molecules (Griffiths et al., 1994), we can propose a similar hypothesis with diatoms. Bacteria associated to diatoms can selectively increase dissolution of silicate minerals (Vandevivere et al., 1994). Diatoms have acquired an ability to exudate specific organic molecules that can increase the dissolution of lithogenic silica and not their own biogenic silica. This strategy could be advantageous during silicate limitation episodes, whereby lithogenic silica becomes a source of silicon. This would be possible if diatoms produce organic ligands with a high affinity for Al. Removal of Al in preference to Si in Al enriched silica would increase lithogenic silica and fossil bSiO2 dissolution without weakening their own frustule.
The main goal of this study was to understand the influence of TEP on biogenic silica dissolution. We used two different types of bSiO2: fossil diatoms (diatomite) and cleaned frustules of cultured diatoms (C. muelleri). Our results evidenced a correlation between TEP concentrations and bSiO2 dissolution rate constants and solubility of the bSiO2 for fossil diatoms, however diatom excretions had no influence on bSiO2 dissolution from freshly cleaned frustules, except for possible protection at the highest TEP concentration.
In the light of the results obtained here, diatom byproducts seem to favor Al-enriched silica dissolution, such as bacterial EPS do for feldspars and aluminosilicates (Welch and Ullman, 1993, 1996; Vandevivere et al., 1994) as compared to fresh bSiO2. In case of limitations, TEP are produced in greater quantities by diatoms that may provide them with an unexpected source of nutrients through the increase of weathering. This would explain the Rare Earth concentrations sometimes measured on diatom frustules and confirm the theory that diatoms may directly uptake silica on lithogenic particles.
JT writing paper, laboratory experiment, sample analysis, data treatment; BM writing paper, support in lab experiment, supervisor.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The reviewer HO and handling Editor declared their shared affiliation.
We would like to thank the Argenton Ifremer experiment station for providing us the algae. We thank Jill Naomi Sutton for her help in editing the English language manuscript, Lucie Blondel for the ICP-AES analysis of diatomite, and everyone else in LEMAR for assistance during the experiment. This work was partly supported by the ANR BIOPSIS project, grant ANR-16-CE01-0002-01 of the French Agence Nationale de la Recherche, and by the CHIBIDO team of the LEMAR.
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Keywords: exopolysaccharide, diatom excretion, diatomite, Chaetoceros muelleri, biogenic silica, bSiO2 dissolution, TEP
Citation: Toullec J and Moriceau B (2018) Transparent Exopolymeric Particles (TEP) Selectively Increase Biogenic Silica Dissolution From Fossil Diatoms as Compared to Fresh Diatoms. Front. Mar. Sci. 5:102. doi: 10.3389/fmars.2018.00102
Received: 08 September 2017; Accepted: 12 March 2018;
Published: 28 March 2018.
Edited by:Toshi Nagata, The University of Tokyo, Japan
Reviewed by:Marta Plavsic, Rudjer Boskovic Institute, Croatia
Hiroshi Ogawa, The University of Tokyo, Japan
Copyright © 2018 Toullec and Moriceau. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jordan Toullec, email@example.com