Frontiers journals are at the top of citation and impact metrics

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Mar. Sci. | doi: 10.3389/fmars.2018.00345

Cellular Bioreactivity of Micro- and Nano- Plastic Particles in Oysters

Tamara Gaspar1,  Richard Chi1, Matthew W. Parrow1 and  Amy H. Ringwood1*
  • 1University of North Carolina at Charlotte, United States

The global usage of plastics has increased dramatically over the last several decades. Polystyrene is the fourth most common plastic material produced annually due to its many versatile applications. Consequently, there has been a coinciding increase in polystyrene wastes, much of which makes its way into waterways and oceanic habitats. While plastic debris has been shown to adversely affect many marine species as a result of ingestion and entanglement, less is known about the cellular uptake of small-scale plastic particles (nano and micro) by marine invertebrates. In this study, we investigated the potential for uptake of polystyrene nano and micron sized beads (50nm and 3um) by the Eastern Oyster, Crassostrea virginica. This research was focused on two key issues: 1) how particle size would affect uptake by hepatopancreas (HP) cells in vitro, and 2) the difference in uptake of micron and nano particles in vivo between gill and HP tissues. This research confirmed that oysters can accumulate polystyrene beads in their tissues, especially HP tissues. Furthermore, using fluorescent deconvolution microscopy, it was observed that plastic nanoparticles exhibited a much greater propensity for intracellular accumulation in HP cells, primarily into lysosomes via endosomal pathways, indicating the potential for significant bioreactivity and sublethal impacts. While exposures of whole oysters or isolated HP cells to bare polystyrene beads did not cause any significant toxicity (acute or sublethal), nanoplastics are more likely to accumulate intracellularly and to deliver adsorbed toxins directly into cells.

Keywords: nanoplastics, Microplastics, Plastics, Nanoparticles, oysters, Lysosomes

Received: 29 Apr 2018; Accepted: 07 Sep 2018.

Edited by:

Juan Jose Alava, University of British Columbia, Canada

Reviewed by:

Ana Tirapé, Escuela Superior Politecnica del Litoral (ESPOL), Ecuador
Gabriela V. Aguirre-Martínez, Facultad de Ciencias de la Salud, Universidad Arturo Prat, Chile  

Copyright: © 2018 Gaspar, Chi, Parrow and Ringwood. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: PhD. Amy H. Ringwood, University of North Carolina at Charlotte, Charlotte, United States, ahringwo@uncc.edu