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Front. Microbiol. | doi: 10.3389/fmicb.2018.00227

Vibrio tapetis displays an original type IV secretion system in strains pathogenic for bivalve molluscs

 Graciela Dias1, 2, Adeline Bidault1,  Patrick Le Chevalier1, 3, Gwenaelle Choquet1,  Clio der Sarkissian4, Ludovic Orlando4, 5, Claudine Medigue6,  Valerie Barbe6,  Fabiano Thompson2,  Cristiane C. Thompson2,  Annick Jacq7,  Vianney Pichereau1 and  Christine Paillard1*
  • 1Laboratoire des Sciences de l'Environenment Marin, Université de Bretagne Occidentale, France
  • 2Institut de Biologia, Universidade Federal do Rio de Janeiro, Brazil
  • 3Laboratoire de Biotechnologie et Chmie Marine, Université de Bretagne Occidentale, France
  • 4Centre for GeoGenetics, University of Copenhagen, Denmark
  • 5Laboratoire d'Anthropobiologie Moléculaire et d'Imagerie de Synthèse, Université Toulouse III Paul Sabatier, France
  • 6CEA, Genoscope, France
  • 7Université Paris-Sud, Université Paris Saclay, CEA, CNRS, UMR9198 Institut de Biologie Intégrative de la Cellule (I2BC), France

The Brown Ring Disease (BRD) caused high mortality rates since 1986 in the Manila clam Venerupis philippinarum introduced and cultured in Western Europe from the 1970s. The causative agent of BRD is a Gram-Negative bacterium, Vibrio tapetis, which is also pathogenic to fish. Here we report the first assembly of the complete genome of V. tapetis CECT4600T, together with the genome sequences of sixteen additional strains isolated across a broad host and geographic range. Our extensive genome dataset allowed us to describe the pathogen pan- and core genomes and to identify putative virulence factors. The V. tapetis core genome consists of 3,352 genes, including multiple potential virulence factors represented by haemolysins, transcriptional regulators, Type I restriction modification system, GGDEF domain proteins, several conjugative plasmids, and a Type IV secretion system. Future research on the coevolutionary arm race between V. tapetis virulence factors and host resistance mechanisms will improve our understanding of how pathogenicity develops in this emerging pathogen.

Keywords: Vibrio tapetis, Venerupis philippinarum, Comparative genomics, pathogenicity, T4SS

Received: 21 Oct 2017; Accepted: 30 Jan 2018.

Edited by:

Feng Gao, Tianjin University, China

Reviewed by:

Stephen L. Michell, University of Exeter, United Kingdom
Chih-Horng Kuo, Institute of Plant and Microbial Biology, Academia Sinica, Taiwan  

Copyright: © 2018 Dias, Bidault, Le Chevalier, Choquet, der Sarkissian, Orlando, Medigue, Barbe, Thompson, Thompson, Jacq, Pichereau and Paillard. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Christine Paillard, Université de Bretagne Occidentale, Laboratoire des Sciences de l'Environenment Marin, Institut Universitaire Européen de la Mer, Brest, France,