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Front. Microbiol. | doi: 10.3389/fmicb.2018.00348

RovM and CsrA Negatively Regulate Urease Expression in Yersinia pseudotuberculosis

 Qingyun Dai1,  Lei Xu1, Lu Xiao1,  Kaixiang Zhu1, Yunhong Song1, Changfu Li1, Lingfang Zhu1,  Xihui Shen1* and  Yao Wang1*
  • 1Northwest A&F University, China

Urease acts as an important acid resistance system and virulence factor that is widespread among microorganisms. RovM is a global regulator that regulates a series of genes and pathways including acid survival systems in the enteric bacterium Yersinia pseudotuberculosis (Yptb). However, whether RovM regulates the urease activity in Yptb was still unknown. In this study, by using qualitative and quantitative urease assays, we show that the urease expression responds to nutrient conditions and the RovM protein represses urease expression by binding to its promoter. A previously reported positive regulator OmpR activates urease activity but RovM plays a dominant role in different nutrient conditions. In addition, carbon storage regulator system A (CsrA), the upstream regulator of RovM, dramatically down-regulates urease activity possibly by its binding to the Shine-Dalgarno (SD) sequence of the mRNA encoding the urease. In conclusion, this study demonstrates that urease activity is strictly controlled by nutrient conditions and is down-regulated by the CsrA-RovM pathway.

Keywords: Urease activity, Yersinia pseudotuberculosis, RovM, Carbon storage regulator system A (CsrA), Nutrients

Received: 02 Dec 2017; Accepted: 13 Feb 2018.

Edited by:

Alain P. Gobert, Vanderbilt University Medical Center, United States

Reviewed by:

Robert Maier, University of Georgia, United States
Michael MARCEAU, Université Lille 2 Droit et Santé, France  

Copyright: © 2018 Dai, Xu, Xiao, Zhu, Song, Li, Zhu, Shen and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Xihui Shen, Northwest A&F University, Xianyang, China,
Dr. Yao Wang, Northwest A&F University, Xianyang, China,