Original Research ARTICLE
LILRB4 Decrease on uDCs Exacerbate Abnormal Pregnancy Outcomes Following Toxoplasma gondii Infection
- 1Department of Gynecology and Obstetrics, Binzhou Medical University Hospital, China
- 2Department of Gynecology and Obstetrics, Yantai Traditional Chinese Medicine Hospital, China
- 3Department of Immunology, Department of Medicine & Pharmacy Research Center, Binzhou Medical University, China
- 4Department of Radiology, Binzhou Medical University Hospital, China
Toxoplasma gondii (T. gondii ) infection in early pregnancy can result in miscarriage, dead fetus, and other abnormalities. The LILRB4 is a central inhibitory receptor in uterine dendritic cells (uDCs) that plays essential immune-regulatory roles at the maternal-fetal interface. In this study, T. gondii-infected human primary uDCs and T. gondii-infected LILRB4−/− pregnant mice were utilized. The immune mechanisms underlying the role of LILRB4 on uDCs were explored in the development of abnormal pregnancy outcomes following T. gondii infection in vitro and in vivo. Our results showed that the expression levels of LILRB4 on uDCs from normal pregnant mice were obviously higher than non-pregnant mice, and peaked in mid-gestation. The LILRB4 expression on uDC subsets, especially tolerogenic subsets, from mid-gestation was obviously down-regulated after T. gondii infection and LILRB4 decrease could further regulate the expression of functional molecules (CD80, CD86, and HLA-DR or MHC II) on uDCs, contributing to abnormal pregnancy outcomes. Our results will shed light on the molecular immune mechanisms of uDCs in abnormal pregnancy outcomes by T. gondii infection.
Keywords: LILRB4, Dendritic Cells, co-stimulatory molecules, Abnormal pregnancy outcomes, Toxoplasma gondii
Received: 14 Dec 2017;
Accepted: 14 Mar 2018.
Edited by:José R. Mineo, Federal University of Uberlandia, Brazil
Reviewed by:Dolores Correa, Instituto Nacional de Pediatria, Mexico
Stephen J. Jordan, University of Alabama at Birmingham, United States
Copyright: © 2018 Zhan, Zheng, Zhang, Zhao, Liu, Jiang, Yang, Ren, Liu and Hu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
MD, PhD. Zhiqiang Liu, Binzhou Medical University Hospital, Department of Gynecology and Obstetrics, Binzhou, 256603, Shandong, China, firstname.lastname@example.org
MD, PhD. Xuemei Hu, Binzhou Medical University, Department of Immunology, Department of Medicine & Pharmacy Research Center, Yantai, 264003, Shandong, China, email@example.com