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Front. Microbiol. | doi: 10.3389/fmicb.2018.02352

Effects of cotton seed powder as the seed medium nitrogen source on the morphology and pneumocandin B0 yield of Glarea lozoyensis

Kai Yuan1, 2, BaoQi Huang1, 2,  Ping Song1, 2*, Ke Zhang1, 2,  Xiao-Jun Ji1, 2,  Lujing Ren1, 2, Sen Zhang3 and  He Huang2*
  • 1College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, China
  • 2School of Pharmaceutical Sciences, Nanjing Tech University, China
  • 3Jiangsu Collaboration Innovation Center of Chinese Medical Resources Industrialization, College of Pharmacy, Nanjing University of Chinese Medicine, China

Pneumocandin B0 is an important antifungal drug precursor produced by filamentous fungus Glarea lozoyensis. The high broth viscosity of cultures of this organism results in lower oxygen solubility and higher energy consumption for agitation and aeration, which mostly caused by the morphologies of filamentous fungi in submerged culture. In this study, the effects of different seed media nitrogen sources on morphology and fermentation behavior of G.lozoyensis were investigated, and cotton seed powder resulted in small, compact pellets. Moreover, pneumocandin B0 yield in Erlenmeyer flasks were increased by 22.9 %. Furthermore, pneumocandin B0 yield in a 50-L fermenter reached 2100 mg/L and the dissolved oxygen maintained above 30 %. Additionally, activities of phosphofructokinase (PFK), isocitrate dehydrogenase (ICDH), glucose 6-phosphatedehydrogenase (G6PDH) and malic enzyme (ME) were increased by 87.5, 50, 41.6 and 10.7 %, respectively. This study demonstrates the feasibility and advantages of using cotton seed powder for controlling the fungal morphology and improving the product yield in pneumocandin fermentations.

Keywords: pneumocandin B0, nitrogen source, morphology, enzyme actibity, dissolved xoygen

Received: 04 Jun 2018; Accepted: 13 Sep 2018.

Edited by:

Weiwen Zhang, Tianjin University, China

Reviewed by:

I-Son Ng, National Cheng Kung University, Taiwan
Guang Zhao, Qingdao Institute of Bioenergy and Bioprocess Technology (CAS), China  

Copyright: © 2018 Yuan, Huang, Song, Zhang, Ji, Ren, Zhang and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Ping Song, Nanjing Tech University, College of Biotechnology and Pharmaceutical Engineering, Nanjing, China, songping@njtech.edu.cn
PhD. He Huang, Nanjing Tech University, School of Pharmaceutical Sciences, Nanjing, China, biotech@njtech.edu.cn