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Front. Microbiol. | doi: 10.3389/fmicb.2019.02657

AiiM lactonase strongly reduces quorum sensing controlled virulence factors in clinical strains of Pseudomonas aeruginosa isolated from burned patients

  • 1Department of Microbiology and Parasitology, Faculty of Medicine, National Autonomous University of Mexico (UNAM), Mexico
  • 2National Institute of Rehabilitation (Mexico), Mexico
  • 3Department of Biochemistry, Faculty of Medicine, National Autonomous University of Mexico, Mexico
  • 4Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Japan
  • 5Genética Molecular, Instituto de Fisiología Celular, UNAM, National Autonomous University of Mexico, Mexico
  • 6Genética Molecular, Instituto de Fisiología Celular, National Autonomous University of Mexico, Mexico

Pseudomonas aeruginosa is an opportunistic bacterium associated with healthcare infections in intensive care units, ventilator-associated pneumonia, surgical site infections and burns. This bacterium causes 75% of death in burned patients, since it can develop a persistent biofilm associated with infections, express several virulence factors and antibiotic-resistance mechanisms. Some of these virulence factors are proteases such as elastase and alkaline protease, or toxic metabolites such as pyocyanin and is one of the few microorganisms able to produce cyanide, which inhibits the cytochrome oxidase of host cells. These virulence factors are controlled by quorum sensing. In this work, 30 P. aeruginosa clinical strains isolated from burned patients from a tertiary hospital in Mexico City were studied. Antibiotic susceptibility tests were done, and virulence factors (elastase, alkaline protease, HCN and pyocyanin) were determined in presence of an N-acylhomoserine lactonase, AiiM able to hydrolyzing a wide range of acyl homoserine lactones. The treatment reduced significantly the activities of elastase and alkaline protease, and the production of pyocyanin and HCN in all producer strains but not the secretion of toxins through the type III secretion system. Our work suggests that AiiM treatment may be an effective therapy to combat P. aeruginosa infection in burn patients.

Keywords: anti-virulence therapy, Burned patients, quorum quenching, Pseudomonas aeruginosa, Virulence Factors, AiiM lactonase

Received: 15 May 2019; Accepted: 31 Oct 2019.

Copyright: © 2019 Lopez-Jacome, Garza Ramos-Martínez, Hernández-Durán, Franco-Cendejas, Romero-Martínez, Thi Nguyen, Maeda, González-Pedrajo, Díaz-Guerrero, Loarca, Sanchéz Reyes, Díaz-Ramírez and García-Contreras. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mx. Rodolfo García-Contreras, Department of Microbiology and Parasitology, Faculty of Medicine, National Autonomous University of Mexico (UNAM), Ciudad de México, Mexico,