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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02661

Tissue specific microbiomes of the Red Sea giant clam Tridacna maxima highlight differential abundance of Endozoicomonadaceae

 Susann Rossbach1*,  Anny Cárdenas1, Gabriela H. Perna1,  Carlos M. Duarte1 and Christian R. Voolstra2
  • 1King Abdullah University of Science and Technology, Saudi Arabia
  • 2Department of Biology, Faculty of Mathematics and Natural Sciences, University of Konstanz, Germany

Giant clams (subfamily Tridacninae) are prevalent members of coral reef communities and engage in symbioses with algal photosymbionts of the family Symbiodiniaceae, similar to their scleractinian coral counterparts. However, we know little about their associated bacterial microbiome members. Here we explored bacterial community diversity of digestive system, gill, and mantle tissues associated with the giant clam Tridacna maxima across a cross-shelf gradient (inshore, midshore, and offshore reef sites) in the central Red Sea using 16S rRNA gene amplicon sequencing. Different tissues harbor spatially stable and distinct microbial communities. Notably, diverse assemblages of bacteria affiliated to the family Endozoicomonadaceae were prevalent in all tissues, but particularly abundant in gills and to a lesser extent in digestive tissues. Besides Endozoicomonadaceae, bacteria in the families Pasteurellaceae, Alteromonadaceae, and Comamonadaceae were common associates, depending on the tissue queried. Taxonomy-based functional inference identified processes related to nitrogen cycling (among others) to be enriched in giant clam tissues and contributed by Endozoicomonadaceae. Our study highlights the tissue-specificity and broad taxonomic range of Endozoicomonadaceae associates, similar to other marine invertebrates, and suggests their contribution to nitrogen-related pathways. The investigation of bivalve-associated microbiome communities provides an important addition to the pathogen-focused studies for commercially important bivalves, (e.g. oysters).

Keywords: microbiome, metabarcoding, coral reef, Symbiosis, Giant clam, Tridacna

Received: 01 Sep 2019; Accepted: 31 Oct 2019.

Copyright: © 2019 Rossbach, Cárdenas, Perna, Duarte and Voolstra. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mrs. Susann Rossbach, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia, susannrossbach@hotmail.com