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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02684

Identification of major capsid protein (MCP) as a potential biomarker of grouper iridovirus infected cells using aptamers selected by SELEX

Qing Yu1, Mingzhu Liu1, Shina Wei2, Hehe Xiao3, Siting Wu2, Ke Ke1,  Xiaohong Huang2*,  Qiwei Qin2* and  Pengfei Li1*
  • 1Guangxi Academy of Sciences, China
  • 2South China Agricultural University, China
  • 3Henan Normal University, China

Biomarkers have important roles in disease pathogenesis, and serve as important disease indicators for developing novel diagnostic and therapeutic approaches. Grouper iridovirus is nucleocytoplasmic DNA viruse, which not only causes great economic losses in the mariculture but also seriously threatens the global biodiversity. However, a lack of biomarkers has limited the progress in clarifying iridovirus pathogenesis. Here, we report novel molecular probes, aptamers, for specific identification of biomarkers in grouper iridovirus-infected cells. Aptamers are selected by SELEX, which is a completely different approach from conventional antibody-based methods for biomarkers discovery. Aptamer-based technology is the unique efficient selection for cell-specific target molecules, and find out new biomarkers without the knowledge of characteristics of proteins expressed on virus-infected cell surface. With the implementation of a two-step strategy (aptamer selection and biomarker discovery), combined with mass spectrometry, grouper iridovirus major capsid protein was ultimately identified as a potential biomarker of aptamer Q5 for grouper iridovirus infection. The specific interactions of aptamer Q5 and MCP were experimentally validated by several assays, including EMSA, co-localization of fluorescence by LSCM, binding competition tests and siRNA silencing tests by flow cytometry. This aptamer-based method for biomarkers discovery developed with grouper iridovirus-infected cells, could be applicable to other types of virus infection, markedly improve our studies of biomarker discovery and virus pathogenesis, and further facilitate the development of diagnostic tools and therapeutic approaches to virus infection.

Keywords: biomarker, Grouper iridovirus, major capsid protein, aptamer, virus pathogenesis

Received: 24 Jul 2019; Accepted: 05 Nov 2019.

Copyright: © 2019 Yu, Liu, Wei, Xiao, Wu, Ke, Huang, Qin and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Xiaohong Huang, South China Agricultural University, Guangzhou, 510642, Guangdong Province, China, huangxh@scau.edu.cn
Mx. Qiwei Qin, South China Agricultural University, Guangzhou, 510642, Guangdong Province, China, qinqw@scsio.ac.cn
Dr. Pengfei Li, Guangxi Academy of Sciences, Nanning, Guangxi Zhuang Region, China, pfli2014@126.com