A central role for ubiquitination within a circadian clock protein modification code
Katarina Stojkovic
Simon S. Wing
Nicolas Cermakian- 1Douglas Mental Health University Institute, McGill University, Montréal, QC, Canada
- 2Polypeptide Laboratory, Department of Medicine–McGill University Health Centre Research Institute, McGill University, Montréal, QC, Canada
Circadian rhythms, endogenous cycles of about 24 h in physiology, are generated by a master clock located in the suprachiasmatic nucleus of the hypothalamus and other clocks located in the brain and peripheral tissues. Circadian disruption is known to increase the incidence of various illnesses, such as mental disorders, metabolic syndrome, and cancer. At the molecular level, periodicity is established by a set of clock genes via autoregulatory translation–transcription feedback loops. This clock mechanism is regulated by post-translational modifications such as phosphorylation and ubiquitination, which set the pace of the clock. Ubiquitination in particular has been found to regulate the stability of core clock components but also other clock protein functions. Mutation of genes encoding ubiquitin ligases can cause either elongation or shortening of the endogenous circadian period. Recent research has also started to uncover roles for deubiquitination in the molecular clockwork. Here, we review the role of the ubiquitin pathway in regulating the circadian clock and we propose that ubiquitination is a key element in a clock protein modification code that orchestrates clock mechanisms and circadian behavior over the daily cycle.
Introduction: The Molecular Circadian Clock
Circadian rhythms are endogenous ∼24 h cycles in physiology and behavior generated by a master clock in the suprachiasmatic nucleus of the hypothalamus, and clocks located in most other tissues. Circadian clocks enable organisms to anticipate predictable daily occurrences, such as changes in light, temperature, or food availability (Dibner et al., 2010). The importance of circadian clocks is illustrated by the impacts of circadian disruption in humans. For example, shift work increases the risk of developing various illnesses, such as mental disorders, metabolic syndrome, and cancer (Evans and Davidson, 2013).
At the molecular level, the circadian clock relies on self-sustained transcription-translation feedback loops involving “clock genes” (Figure 1; Duguay and Cermakian, 2009). In mammals, CLOCK and BMAL1 dimerize and activate Period (Per) 1 and 2 and Cryptochrome (Cry) 1 and 2 genes. The PER1/2 and CRY1/2 proteins then enter the nucleus and inhibit the activity of CLOCK/BMAL1, thereby repressing their own transcription. However, the mechanism is more complex, with additional interlocking feedback loops, including one that involves the induction of the Rev-erb and Ror genes, whose protein products regulate Bmal1 gene transcription. One consequence of these feedback loops is that the mRNAs and proteins of many clock genes present circadian rhythms in their abundance. Moreover, hundreds of clock-controlled genes, which do not participate in the clock mechanism, but whose transcription is under the control of the clock molecular machinery, also present rhythms at the RNA and protein levels (Storch et al., 2002; Yan et al., 2008), thus linking the circadian clock with cellular physiology.
FIGURE 1. Simplified molecular mechanism of the mammalian circadian clock. The transcription factors CLOCK and BMAL1 activate the expression of Per and Cry genes through E-box elements in their promoters. PER and CRY proteins form complexes and feedback negatively on CLOCK/BMAL1 activity, and thus, on their own expression. CLOCK/BMAL1 also activate genes encoding nuclear receptors of the REV-ERB and ROR families, which regulate the expression of Bmal1 (and also Cry1 and Clock). For each protein or pair of proteins, the colored boxes list the post-translational modifications (PTMs) that have been identified so far, as well as the ubiquitin-modifying enzymes shown to be involved (HUWE1, PAM, β-TRCP1/2 are also called ARF-BP1, MYCBP2, FBW1A/B, respectively). For simplicity, a single box per protein is shown, irrespective of the subcellular localization of the PTMs. P, phosphorylation; Ub, ubiquitination; Ac, acetylation; SUMO, SUMOylation; O-GlcNAc, addition of β-D-N-acetylglucosamine; S-Ni, S-nitrosylation; ADPrib, ADP-ribosylation; PER, Period; CRY, Cryptochrome; ROR, Retinoic acid receptor-related orphan receptor; DUB, deubiquitinating enzyme.
The timing of these feedback loops is dictated by post-translational modifications (PTMs; Gallego and Virshup, 2007; Duguay and Cermakian, 2009). Indeed, clock proteins are subject to phosphorylation, ubiquitination, acetylation, SUMOylation, and other PTMs (Figure 1). Ubiquitination is of particular interest due to the diversity of signals that it can generate. In particular, its direct role in determining protein half-life is crucial for proteins with a daily rhythm in abundance. In this article, we review the current state of knowledge on ubiquitination of clock proteins and their ubiquitin-modifying enzymes in animal models, with a special focus on the mammalian clock (Table 1).
TABLE 1. Ubiquitin-modifying enzymes involved in the regulation of mammalian clock proteins.
Ubiquitination in the Circadian Clock
Ubiquitination of Cryptochromes by FBXL Ubiquitin Ligases
N-Ethyl-N-nitrosourea screens led to the discovery of mice exhibiting free-running periods of locomotor activity rhythms ∼2–3 h longer than normal (Godinho et al., 2007; Siepka et al., 2007). These mice had loss-of-function mutations in the gene encoding the F-box protein FBXL3. Loss of FBXL3 activity leads to CRY protein stabilization due to decreased ubiquitination (Siepka et al., 2007). Further work on FBXL3 revealed that ubiquitination of CRY1/2 by the FBXL3-containing SCF E3 ubiquitin ligase complex was necessary for the timely degradation of the CRY proteins and the reactivation of BMAL1/CLOCK (Busino et al., 2007). A prolonged inhibition of BMAL1/CLOCK-mediated transcription in the mutant mice leads to reduced peak levels and delayed rhythms of the Per and Cry mRNAs in mutant mouse SCN, cerebellum, and liver (Godinho et al., 2007; Siepka et al., 2007).
Interestingly, FBXL3 cannot undergo SCF complex formation in the absence of its CRY substrates (Yumimoto et al., 2013). X-ray crystallography revealed that FBXL3 binds to the FAD-binding pocket of mammalian CRY, which may also be bound by FAD or PER proteins (Czarna et al., 2013; Xing et al., 2013), which suggests a mechanism for the protection of CRYs from degradation in the presence of PER (Yagita et al., 2002).
An FBXL3 paralog, FBXL21, was identified in sheep, where it was also found to bind to CRY1, thereby affecting transcriptional activation by CLOCK/BMAL1 (Dardente et al., 2008). Despite the high similarity between FBXL3 and FBXL21, they appear to have non-redundant roles within the clock. Indeed, while Fbxl3 gene mutant or knock-out (KO) mice display a long free-running period of locomotor activity rhythms, Fbxl21-mutant or KO mice present either a short (Yoo et al., 2013) or a normal (Hirano et al., 2013) period. Moreover, when the mutant lines are crossed, the Fbxl21 mutation attenuates the long-period phenotype of Fbxl3-mutant mice.
The distinct roles of the FBXL proteins may be based on the timing of their expression and that of their substrates. While Fbxl3 is expressed at constant levels over the day, Fbxl21 expression has a pronounced circadian rhythm in the mouse SCN, with a peak by the end of the subjective day (Dardente et al., 2008), thus restricting its action to only part of the cycle. Interestingly, while FBXL3 protein levels do not vary over time, its action on CRYs is conditional on their phosphorylation by AMPK, whose expression and nuclear abundance vary over the day (Lamia et al., 2009). Ligase intracellular localization also plays a role: while FBXL3 protein is restricted to the nucleus, FBXL21 is located both in the nucleus and cytoplasm (Hirano et al., 2013; Yoo et al., 2013). The work of both laboratories supports a two-step mode of action of FBXL21. First, FBXL21 allows CRYs to accumulate in the cytoplasm. This occurs when CRY levels rise around the end of the day or beginning of the night. Shortly thereafter, after CRYs have entered the nucleus, FBXL21 might counteract FBXL3: FBXL21 binds CRYs more stably and with a higher affinity than FBXL3, suggesting that FBXL21 may in part stabilize CRYs by preventing FBXL3 binding (Yoo et al., 2013). Then, when FBXL21 levels have decreased, FBXL3 can finally act on CRYs and target them to degradation. It is interesting to note that if this model is further confirmed, the roles of FBXL21 in the clock will turn out to be partly non-degradative (regulation of nuclear entry, protection from the action of another F-box protein, FBXL3), in contrast to other ubiquitin ligases involved in the clock, which target clock proteins to proteasomal degradation. Finally, CRY ubiquitination mechanisms might be even more complex, as it was suggested that another ubiquitin ligase might be involved in regulating CRY accumulation (Kurabayashi et al., 2010; Hirano et al., 2013).
Ubiquitination of Period Proteins by β-TRCP Ubiquitin Ligases
In Drosophila, the F-box component of an SCF ligase, SLIMB, was shown to be critical for ubiquitination and degradation of PER protein over the course of the circadian cycle (Grima et al., 2002; Ko et al., 2002). SLIMB binds to PER after its phosphorylation by Doubletime (DBT), in particular on serine 47 within the SLIMB recognition site (Chiu et al., 2008).
SLIMB has two homologs in mammals, β-TRCP1/FBW1A and β-TRCP2/FBW1B. Similarly to the action of DBT and SLIMB on PER in the fly, β-TRCP1/2 are recruited to PER2 following phosphorylation of this protein by the kinases CK1δ and CK1ε (mammalian homologs of DBT), which leads to polyubiquitination and subsequent degradation of PER2 (Eide et al., 2005). Indeed, expression of a dominant negative form of β-TRCP leads to the inhibition of PER2 ubiquitination and degradation. β-TRCP1/2 also interact with PER1 in a CK1ε-dependent manner and a knockdown of both β-TRCPs was found to stabilize PER1, and reduce levels of transcriptional activation by CLOCK/BMAL1 (Shirogane et al., 2005). Accordingly, preventing the action of β-TRCP1/2 on PER proteins leads to long-period or dampened circadian rhythms in cultured fibroblasts (Reischl et al., 2007; Ohsaki et al., 2008). Surprisingly though, mice lacking β-TRCP1 neither show alteration in circadian locomotor behavior nor differences in SCN PER2 levels when compared to WT controls, suggesting either that the SCN clock behaves differently from clocks in fibroblasts or that there is redundancy at the level of the ubiquitin ligases (Ohsaki et al., 2008). Finally, similarly to PER proteins protecting CRYs (see Ubiquitination of Cryptochromes by FBXL Ubiquitin Ligases), PER proteins are protected from ubiquitination and degradation upon association with CRYs (Yagita et al., 2002).
Ubiquitination of REV-ERBα
The stability of REV-ERBα is also regulated by a sequence of phosphorylation, ubiquitination, and proteasomal degradation. Indeed, REV-ERBα is stabilized following phosphorylation by GSK3β (Yin et al., 2006). Treating cells with lithium, a GSK3β inhibitor, leads to the quick degradation of REV-ERBα and therefore, to increased expression of Bmal1 (Figure 1). Subsequent work identified HUWE1/ARF-BP1 and PAM/MYCBP2 as E3 ligases involved in this lithium-induced REV-ERBα degradation (Yin et al., 2010). Their depletion in cells stabilized REV-ERBα, decreased Bmal1 gene expression, and disrupted oscillations of other clock genes. HUWE1 and PAM may not be the only ubiquitin ligases acting on REV-ERBα. In Fbxl3-mutant mice, REV-ERBα levels are higher and consequently its repression of Bmal1 and Cry1 genes is enhanced. Creation of double-mutant Fbxl3/Rev-erbα-/- mice rescues the Fbxl3-mutant phenotype (Shi et al., 2013) indicating that FBXL3, in addition to its role on CLOCK/BMAL1-mediated transcription via destabilization of CRYs, also has an effect on REV-ERBα-mediated repression of target genes. Although this effect may be indirect, it does indicate a role for this F-box protein as a coordinator of different clock transcription factors.
Ubiquitination of BMAL1
Many studies have indicated a tight regulation of BMAL1 stability. Indeed, BMAL1 undergoes different phosphorylation events that either target it for ubiquitination and degradation (e.g., GSK3β, Sahar et al., 2010) or on the contrary for deubiquitination and stabilization (e.g., PKCγ, Zhang et al., 2012). Importantly, BMAL1 ubiquitination and proteasome-mediated proteolysis appear to coincide with the time of highest transcriptional activity (Kwon et al., 2006; Lee et al., 2008; Stratmann et al., 2012), whereas in conditions where CLOCK/BMAL1 activity is repressed (e.g., presence of CRYs), BMAL1 is stabilized (Kondratov et al., 2006; Dardente et al., 2007). However, no BMAL1-specific ubiquitin ligase had been uncovered until a recent report, which described UBE3A as an E3 ligase that binds and destabilizes BMAL1 (Gossan et al., 2014). Knockdown of this ligase in mammalian cells and in Drosophila clock neurons leads to a strong dampening of circadian oscillations or even arrhythmicity.
SUMOylation in the Circadian Clock
The small ubiquitin-related modifier (SUMO) proteins also play a role in the clock. Like other PTMs, SUMOylation is reversible and the conjugation/deconjugation mechanisms are reminiscent of the ubiquitin pathway (Muller et al., 2001). In contrast to ubiquitination though, SUMOylation does not directly target proteins for degradation but rather regulates other functions such as nuclear localization, protein–protein interactions, transcriptional activity and, interestingly, ubiquitination itself (Desterro et al., 1998; Buschmann et al., 2000).
SUMOylation was first implicated in the clock following the discovery of a SUMOylation consensus motif in BMAL1 (Cardone et al., 2005). Co-expression of BMAL1 and SUMO showed that BMAL1 could indeed be SUMOylated. In the liver, this occurs in a rhythmic manner, with peak SUMOylation in the second half of the light phase. This timing coincides with peak BMAL1 phosphorylation and activity, suggesting an interplay between these PTMs. In further support of this, a functional CLOCK protein is required for both BMAL1 SUMOylation and phosphorylation (Kondratov et al., 2003; Cardone et al., 2005; Dardente et al., 2007). SUMOylated BMAL1 is most abundant when the CLOCK/BMAL1 targets Dbp and Rev-erbα show their highest mRNA levels, again supporting that SUMOylation of BMAL1 is involved in its transcriptional activity (Lee et al., 2008). Indeed, BMAL1 binding to the Dbp promoter was reduced when the lysine required for SUMOylation was mutated (Lee et al., 2008). Interestingly, SUMOylation of BMAL1 is a prerequisite for its subsequent ubiquitination, again highlighting the interplay of different PTMs in the circadian clock.
Deubiquitination in the Circadian Clock
Given the importance of ubiquitination within the clock, it appears reasonable to assume that deubiquitination plays a role as well. Interestingly, the mRNA levels of a deubiquitinating enzyme (DUB), ubiquitin-specific protease 2 (USP2), show rhythmicity in most tissues examined (Kita et al., 2002; Storch et al., 2002; Yan et al., 2008). This is notable, because among the hundreds of clock-controlled transcripts, only a small minority cycles in multiple locations. The circadian rhythm of Usp2 is blunted in Clock mutant and Bmal1 KO mice (Oishi et al., 2003; Molusky et al., 2012b), and the Usp2 promoter is activated by CLOCK/BMAL1 (Molusky et al., 2012b), indicating that Usp2 is a direct target of these transcription factors. In addition to its circadian regulation, Usp2 expression is also induced by starvation and it was therefore proposed that USP2 integrates nutritional and circadian timing cues (Molusky et al., 2012b). In turn, liver USP2 appears to be involved in the generation of a diurnal rhythm in glucose metabolism (Molusky et al., 2012a).
However, the circadian role of USP2 is not limited to mediating the rhythmic control of cellular processes by the molecular clock. Since the short list of genes rhythmic in multiple tissues is enriched for clock components, USP2 was hypothesized to exert a role within the clock mechanism. To address this, Usp2 KO mice were generated by two laboratories. In one case, they revealed no alteration of the free-running period of locomotor rhythms (Scoma et al., 2011). In contrast, our Usp2 KOs display a period longer than WT littermates (Yang et al., 2012), implying a role within the clockwork. In line with this, the absence of USP2 affects the mRNA levels of several clock genes (Scoma et al., 2011; Yang et al., 2012, 2014), and USP2 interacts with clock proteins. In our hands, whereas it forms a complex with several clock proteins, USP2 directly binds only to PER1 (Yang et al., 2012). Accordingly, PER1 is deubiquitinated in the presence of USP2, but notably, this does not lead to PER1 stabilization. Instead, USP2 appears to regulate PER1 intracellular localization (Yang et al., 2014). Interestingly, the only other DUB that to our knowledge has been implicated in clock mechanisms, Drosophila USP8, also seems to act in a non-degradative manner: it deubiquitinates CLOCK, thereby inhibiting transcriptional activity of CLOCK/CYCLE (CYCLE is the Drosophila homolog of BMAL1; Luo et al., 2012).
In contrast, the work of other groups showed a stabilization of other clock proteins due to deubiquitination by USP2. BMAL1 levels are lower in the SCN of Usp2 KO mice (Scoma et al., 2011), whereas in cultured cells, USP2 stabilized BMAL1 (Scoma et al., 2011) and reduced its ubiquitination (Lee et al., 2008). Interestingly, a report suggested the involvement of PKCγ-triggered deubiquitination of BMAL1 in the resetting of peripheral clocks by feeding schedules, but the DUB involved in this pathway remains unknown (Zhang et al., 2012). In addition to PER1 and BMAL1, USP2 deubiquitinates CRY1 in cultured cells in response to a serum shock, and in the mouse liver, Usp2 knockdown increases CRY ubiquitination and decreases CRY1 protein levels (Tong et al., 2012).
Data also support a role for USP2 in the response of the clock to external cues. We found that Usp2 KO mice exhibit larger phase delays than WT mice after light treatment in the first part of the night, and reduced phase advances, upon light treatment later in the night (Yang et al., 2012). Thus, USP2 appears to be involved in the response of the SCN clock to light, which is also supported by data of Scoma et al. (2011), which show increased phase-shifting in response to low irradiance light in the early night. USP2 may also mediate the response of the clock to inflammation, as the expression of the gene is increased in response to TNFα treatment, and CRY1 protein induction in response to this cytokine is abrogated when Usp2 expression is knocked down (Tong et al., 2012).
Together, these studies ascribe a pivotal role to USP2, and deubiquitination in general, not only in the circadian clock mechanism, but also as an integrator of environmental and physiological signals, and in output pathways linking the molecular clockwork to cellular and physiological functions.
A Clock Protein Modification Code?
Overall, the work described above underscores the importance of PTMs within the circadian timing mechanism. Given that different modifications often converge on the same clock protein, we propose the existence of a clock protein modification code whereby the fate/function of a given protein is determined by the precise combination and/or the consecutive occurrence of different PTMs. This clock protein modification code is proposed to exist at different levels:
- Interplay of different PTMs: PTMs often occur sequentially. In particular, there are numerous examples of phosphorylation at specific sites being a pre-requisite for subsequent ubiquitination of the target protein (Cardozo and Pagano, 2004), as occurs in many clock proteins. Another example of sequential modification is the SUMOylation of BMAL1 as a pre-requisite for its ubiquitination (Lee et al., 2008). Moreover, different combinations of PTMs on a protein can lead to distinct outcomes. For example, dual SUMOylation and ubiquitination of BMAL1 result in the BMAL1 localization to the nuclear bodies and active transcription (Lee et al., 2008), whereas later in the circadian cycle, additional events, possibly including further ubiquitination, lead to degradation of the protein. Combinations of different phosphorylation events can also regulate protein fate differentially: for example, in Drosophila, PER phosphorylation by DBT is modulated by prior action of another kinase, NEMO, and consequently, these kinases have opposing effects on PER stability (Chiu et al., 2011).
- Ubiquitin code: There is a large diversity in the ubiquitination of proteins (Heride et al., 2014): they can be mono- or polyubiquitinated; in the latter case, ubiquitin chains can be linear or branched, and the linkages between ubiquitin monomers can be via different lysines. These different ubiquitination states can be generated by various ligases/conjugating enzymes and DUBs. This ubiquitin code can be read by proteins containing ubiquitin-binding domains (UBDs). The effects of ubiquitination can therefore be diverse depending on the type of modifications and the presence of particular UBD-containing proteins: not only targeting to the proteasome, but also regulation of intracellular localization, activity, protein–protein interaction, etc. (Komander et al., 2009). As non-degradative functions of clock protein ubiquitination have started to be identified (see previous sections), it is now important to characterize precisely the ubiquitin code (location and type of ubiquitination) on clock proteins and identify the specific UBD-containing proteins that recognize the code and translate it into specific effects on clock proteins.
- PTMs around the clock: PTMs of clock proteins are orchestrated across the 24 h cycle. For example, BMAL1 undergoes a series of PTMs associated with a variation in activity and partner binding. Peak phosphorylation and SUMOylation of BMAL1 occurs in the late subjective day in mouse peripheral tissues (Cardone et al., 2005; Lee et al., 2008), and SUMOylation is a pre-requisite for ubiquitination (Lee et al., 2008). The occurrence of these PTMs coincides with peak transcriptional activity of the CLOCK/BMAL1 dimer (Ripperger and Schibler, 2006; Stratmann et al., 2012). Further, this appears to be regulated by another PTM, O-GlcNAcylation, which opposes the ubiquitination of BMAL1 (Li et al., 2013). This maximal activity of CLOCK/BMAL1 results in expression of CRY proteins that then repress CLOCK/BMAL1, at a time that is synchronous with the BMAL1 dephosphorylation and stabilization (Kwon et al., 2006; Dardente et al., 2007). BMAL1 acetylation by CLOCK also occurs at this time and leads to increased recruitment of CRY (Hirayama et al., 2007). CRYs themselves are good examples of substrates for sequential PTMs over the 24 h day and across the progression of the clock feedback loop (see Ubiquitination of Cryptochromes by FBXL Ubiquitin Ligases). Therefore, each clock protein undergoes a daily wave of PTMs, in a sequential and often conditional manner, which determines the expression, localization, and activity of the protein and its partners.
Conclusion
In conclusion, ubiquitination and deubiquitination are involved in the regulation of key core clock components. On one hand, ubiquitin ligases are selectively acting on one or a few clock proteins. A given clock protein can even be the target of two or three different E3 ligases, depending on the time of day and cellular compartment. On the other hand, DUBs seem less specific, and only one was identified as a mammalian clock component so far: USP2. This DUB regulates the stability and function of PER1, CRY1, BMAL1 and perhaps other clock proteins, as well as components of the input and output pathways of the clock. Moreover, there is a complex interplay of ubiquitination with other PTMs. It will be crucial in future years to precisely define ubiquitin chain configurations and conjugation sites on clock proteins, to unravel the precise regulation of their addition and removal and identify all the actors involved. Furthermore, ubiquitination becomes an attractive drug target. Indeed, recent chemical screens of compounds binding CRY proteins have identified molecules modulating their ubiquitin-induced degradation (Hirota et al., 2012), suggesting the possibility of therapeutic resetting of the circadian clock by drug-mediated ubiquitin modulation of clock components.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
The authors thank Dr. Kai-Florian Storch for critical review of the manuscript and all members of Nicolas Cermakian’s laboratory for discussions. This work was supported by grants from the Natural Sciences and Engineering Research Council (RGPIN 249731-12) to Nicolas Cermakian and the Canadian Institutes of Health Research (MOP 115106) to Simon S. Wing. Katarina Stojkovic was supported by a fellowship from McGill Faculty of Medicine and Nicolas Cermakian by a salary award from the Fonds de Recherche du Québec—Santé.
References
Buschmann, T., Fuchs, S. Y., Lee, C. G., Pan, Z. Q., and Ronai, Z. (2000). SUMO-1 modification of Mdm2 prevents its self-ubiquitination and increases Mdm2 ability to ubiquitinate p53. Cell 101, 753–762. doi: 10.1016/S0092-8674(00)80887-9
Busino, L., Bassermann, F., Maiolica, A., Lee, C., Nolan, P. M., Godinho, S. I.,et al. (2007). SCFFbxl3 controls the oscillation of the circadian clock by directing the degradation of cryptochrome proteins. Science 316, 900–904. doi: 10.1126/science.1141194
Cardone, L., Hirayama, J., Giordano, F., Tamaru, T., Palvimo, J. J., and Sassone-Corsi, P. (2005). Circadian clock control by SUMOylation of BMAL1. Science 309, 1390–1394. doi: 10.1126/science.1110689
Cardozo, T., and Pagano, M. (2004). The SCF ubiquitin ligase: insights into a molecular machine. Nat. Rev. Mol. Cell Biol. 5, 739–751. doi: 10.1038/nrm1471
Chiu, J. C., Ko, H. W., and Edery, I. (2011). NEMO/NLK phosphorylates PERIOD to initiate a time-delay phosphorylation circuit that sets circadian clock speed. Cell 145, 357–370. doi: 10.1016/j.cell.2011.04.002
Chiu, J. C., Vanselow, J. T., Kramer, A., and Edery, I. (2008). The phospho-occupancy of an atypical SLIMB-binding site on PERIOD that is phosphorylated by DOUBLETIME controls the pace of the clock. Genes Dev. 22, 1758–1772. doi: 10.1101/gad.1682708
Czarna, A., Berndt, A., Singh, H. R., Grudziecki, A., Ladurner, A. G., Timinszky, G.,et al. (2013). Structures of Drosophila cryptochrome and mouse cryptochrome1 provide insight into circadian function. Cell 153, 1394–1405. doi: 10.1016/j.cell.2013.05.011
Dardente, H., Fortier, E. E., Martineau, V., and Cermakian, N. (2007). Cryptochromes impair phosphorylation of transcriptional activators in the clock: a general mechanism for circadian repression. Biochem. J. 402, 525–536. doi: 10.1042/BJ20060827
Dardente, H., Mendoza, J., Fustin, J. M., Challet, E., and Hazlerigg, D. G. (2008). Implication of the F-Box Protein FBXL21 in circadian pacemaker function in mammals. PLoS ONE 3:e3530. doi: 10.1371/journal.pone.0003530
Desterro, J. M., Rodriguez, M. S., and Hay, R. T. (1998). SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation. Mol. Cell 2, 233–239. doi: 10.1016/S1097-2765(00)80133-1
Dibner, C., Schibler, U., and Albrecht, U. (2010). The mammalian circadian timing system: organization and coordination of central and peripheral clocks. Annu. Rev. Physiol. 72, 517–549. doi: 10.1146/annurev-physiol-021909-135821
Duguay, D., and Cermakian, N. (2009). The crosstalk between physiology and circadian clock proteins. Chronobiol. Int. 26, 1479–1513. doi: 10.3109/07420520903497575
Eide, E. J., Woolf, M. F., Kang, H., Woolf, P., Hurst, W., Camacho, F.,et al. (2005). Control of mammalian circadian rhythm by CKIepsilon-regulated proteasome-mediated PER2 degradation. Mol. Cell. Biol. 25, 2795–2807. doi: 10.1128/MCB.25.7.2795-2807.2005
Evans, J. A., and Davidson, A. J. (2013). Health consequences of circadian disruption in humans and animal models. Prog. Mol. Biol. Transl. Sci. 119, 283–323. doi: 10.1016/B978-0-12-396971-2.00010-5
Gallego, M., and Virshup, D. M. (2007). Post-translational modifications regulate the ticking of the circadian clock. Nat. Rev. Mol. Cell Biol. 8, 139–148. doi: 10.1038/nrm2106
Godinho, S. I., Maywood, E. S., Shaw, L., Tucci, V., Barnard, A. R., Busino, L.,et al. (2007). The after-hours mutant reveals a role for Fbxl3 in determining mammalian circadian period. Science 316, 897–900. doi: 10.1126/science. 1141138
Gossan, N. C., Zhang, F., Guo, B., Jin, D., Yoshitane, H., Yao, A.,et al. (2014). The E3 ubiquitin ligase UBE3A is an integral component of the molecular circadian clock through regulating the BMAL1 transcription factor. Nucleic Acids Res. 42, 5765–5775. doi: 10.1093/nar/gku225
Grima, B., Lamouroux, A., Chelot, E., Papin, C., Limbourg-Bouchon, B., and Rouyer, F. (2002). The F-box protein slimb controls the levels of clock proteins period and timeless. Nature 420, 178–182. doi: 10.1038/nature01122
Heride, C., Urbe, S., and Clague, M. J. (2014). Ubiquitin code assembly and disassembly. Curr. Biol. 24, R215–R220. doi: 10.1016/j.cub.2014.02.002
Hirano, A., Yumimoto, K., Tsunematsu, R., Matsumoto, M., Oyama, M., Kozuka-Hata, H.,et al. (2013). FBXL21 regulates oscillation of the circadian clock through ubiquitination and stabilization of cryptochromes. Cell 152, 1106–1118. doi: 10.1016/j.cell.2013.01.054
Hirayama, J., Sahar, S., Grimaldi, B., Tamaru, T., Takamatsu, K., Nakahata, Y.,et al. (2007). CLOCK-mediated acetylation of BMAL1 controls circadian function. Nature 450, 1086–1090. doi: 10.1038/nature06394
Hirota, T., Lee, J. W., St. John, P. C., Sawa, M., Iwaisako, K., Noguchi, T.,et al. (2012). Identification of small molecule activators of cryptochrome. Science 337, 1094–1097. doi: 10.1126/science.1223710
Kita, Y., Shiozawa, M., Jin, W., Majewski, R. R., Besharse, J. C., Greene, A. S.,et al. (2002). Implications of circadian gene expression in kidney, liver and the effects of fasting on pharmacogenomic studies. Pharmacogenetics 12, 55–65. doi: 10.1097/00008571-200201000-00008
Ko, H. W., Jiang, J., and Edery, I. (2002). Role for slimb in the degradation of Drosophila period protein phosphorylated by doubletime. Nature 420, 673–678. doi: 10.1038/nature01272
Komander, D., Clague, M. J., and Urbe, S. (2009). Breaking the chains: structure and function of the deubiquitinases. Nat. Rev. Mol. Cell Biol. 10, 550–563. doi: 10.1038/nrm2731
Kondratov, R. V., Chernov, M. V., Kondratova, A. A., Gorbacheva, V. Y., Gudkov, A. V., and Antoch, M. P. (2003). BMAL1-dependent circadian oscillation of nuclear CLOCK: posttranslational events induced by dimerization of transcriptional activators of the mammalian clock system. Genes Dev. 17, 1921–1932. doi: 10.1101/gad.1099503
Kondratov, R. V., Kondratova, A. A., Lee, C., Gorbacheva, V. Y., Chernov, M. V., and Antoch, M. P. (2006). Post-translational regulation of circadian transcriptional CLOCK(NPAS2)/BMAL1 complex by CRYPTOCHROMES. Cell Cycle 5, 890–895. doi: 10.4161/cc.5.8.2684
Kurabayashi, N., Hirota, T., Sakai, M., Sanada, K., and Fukada, Y. (2010). DYRK1A and glycogen synthase kinase 3beta, a dual-kinase mechanism directing proteasomal degradation of CRY2 for circadian timekeeping. Mol. Cell. Biol. 30, 1757–1768. doi: 10.1128/MCB.01047-09
Kwon, I., Lee, J., Chang, S. H., Jung, N. C., Lee, B. J., Son, G. H.,et al. (2006). BMAL1 shuttling controls transactivation and degradation of the CLOCK/BMAL1 heterodimer. Mol. Cell. Biol. 26, 7318–7330. doi: 10.1128/MCB.00337-06
Lamia, K. A., Sachdeva, U. M., Ditacchio, L., Williams, E. C., Alvarez, J. G., Egan, D. F.,et al. (2009). AMPK regulates the circadian clock by cryptochrome phosphorylation and degradation. Science 326, 437–440. doi: 10.1126/science.1172156
Lee, J., Lee, Y., Lee, M. J., Park, E., Kang, S. H., Chung, C. H.,et al. (2008). Dual modification of BMAL1 by SUMO2/3 and ubiquitin promotes circadian activation of the CLOCK/BMAL1 complex. Mol. Cell. Biol. 28, 6056–6065. doi: 10.1128/MCB.00583-08
Li, M. D., Ruan, H. B., Hughes, M. E., Lee, J. S., Singh, J. P., Jones, S. P.,et al. (2013). O-GlcNAc signaling entrains the circadian clock by inhibiting BMAL1/CLOCK ubiquitination. Cell Metab. 17, 303–310. doi: 10.1016/j.cmet.2012.12.015
Luo, W., Li, Y., Tang, C. H., Abruzzi, K. C., Rodriguez, J., Pescatore, S.,et al. (2012). CLOCK deubiquitylation by USP8 inhibits CLK/CYC transcription in Drosophila. Genes Dev. 26, 2536–2549. doi: 10.1101/gad.200584.112
Molusky, M. M., Li, S., Ma, D., Yu, L., and Lin, J. D. (2012a). Ubiquitin-specific protease 2 regulates hepatic gluconeogenesis and diurnal glucose metabolism through 11beta-hydroxysteroid dehydrogenase 1. Diabetes 61, 1025–1035. doi: 10.2337/db11-0970
Molusky, M. M., Ma, D., Buelow, K., Yin, L., and Lin, J. D. (2012b). Peroxisomal localization and circadian regulation of ubiquitin-specific protease 2. PLoS ONE 7:e47970. doi: 10.1371/journal.pone.0047970
Muller, S., Hoege, C., Pyrowolakis, G., and Jentsch, S. (2001). SUMO, ubiquitin’s mysterious cousin. Nat. Rev. Mol. Cell Biol. 2, 202–210. doi: 10.1038/35056591
Ohsaki, K., Oishi, K., Kozono, Y., Nakayama, K., Nakayama, K. I., and Ishida, N. (2008). The role of {beta}-TrCP1 and {beta}-TrCP2 in circadian rhythm generation by mediating degradation of clock protein PER2. J. Biochem. 144, 609–618. doi: 10.1093/jb/mvn112
Oishi, K., Miyazaki, K., Kadota, K., Kikuno, R., Nagase, T., Atsumi, G.,et al. (2003). Genome-wide expression analysis of mouse liver reveals CLOCK-regulated circadian output genes. J. Biol. Chem. 278, 41519–41527. doi: 10.1074/jbc.M304564200
Reischl, S., Vanselow, K., Westermark, P. O., Thierfelder, N., Maier, B., Herzel, H.,et al. (2007). Beta-TrCP1-mediated degradation of PERIOD2 is essential for circadian dynamics. J. Biol. Rhythms 22, 375–386. doi: 10.1177/0748730407303926
Ripperger, J. A., and Schibler, U. (2006). Rhythmic CLOCK-BMAL1 binding to multiple E-box motifs drives circadian Dbp transcription and chromatin transitions. Nat. Genet. 38, 369–374. doi: 10.1038/ng1738
Sahar, S., Zocchi, L., Kinoshita, C., Borrelli, E., and Sassone-Corsi, P. (2010). Regulation of BMAL1 protein stability and circadian function by GSK3beta-mediated phosphorylation. PLoS ONE 5:e8561. doi: 10.1371/journal.pone.0008561
Scoma, H. D., Humby, M., Yadav, G., Zhang, Q., Fogerty, J., and Besharse, J. C. (2011). The de-ubiquitinylating enzyme, USP2, is associated with the circadian clockwork and regulates its sensitivity to light. PLoS ONE 6:e25382. doi: 10.1371/journal.pone.0025382
Shi, G., Xing, L., Liu, Z., Qu, Z., Wu, X., Dong, Z.,et al. (2013). Dual roles of FBXL3 in the mammalian circadian feedback loops are important for period determination and robustness of the clock. Proc. Natl. Acad. Sci. U.S.A. 110, 4750–4755. doi: 10.1073/pnas.1302560110
Shirogane, T., Jin, J., Ang, X. L., and Harper, J. W. (2005). SCFbeta-TRCP controls clock-dependent transcription via casein kinase 1-dependent degradation of the mammalian period-1 (Per1) protein. J. Biol. Chem. 280, 26863–26872. doi: 10.1074/jbc.M502862200
Siepka, S. M., Yoo, S. H., Park, J., Song, W., Kumar, V., Hu, Y.,et al. (2007). Circadian mutant overtime reveals F-box protein FBXL3 regulation of cryptochrome and period gene expression. Cell 129, 1011–1023. doi: 10.1016/j.cell.2007.04.030
Storch, K. F., Lipan, O., Leykin, I., Viswanathan, N., Davis, F. C., Wong, W. H.,et al. (2002). Extensive and divergent circadian gene expression in liver and heart. Nature 417, 78–83. doi: 10.1038/nature744
Stratmann, M., Suter, D. M., Molina, N., Naef, F., and Schibler, U. (2012). Circadian Dbp transcription relies on highly dynamic BMAL1-CLOCK interaction with E boxes and requires the proteasome. Mol. Cell 48, 277–287. doi: 10.1016/j.molcel.2012.08.012
Tong, X., Buelow, K., Guha, A., Rausch, R., and Yin, L. (2012). USP2a protein deubiquitinates and stabilizes the circadian protein CRY1 in response to inflammatory signals. J. Biol. Chem. 287, 25280–25291. doi: 10.1074/jbc.M112.340786
Xing, W., Busino, L., Hinds, T. R., Marionni, S. T., Saifee, N. H., Bush, M. F.,et al. (2013). SCF(FBXL3) ubiquitin ligase targets cryptochromes at their cofactor pocket. Nature 496, 64–68. doi: 10.1038/nature11964
Yagita, K., Tamanini, F., Yasuda, M., Hoeijmakers, J. H., Van Der Horst, G. T., and Okamura, H. (2002). Nucleocytoplasmic shuttling and mCRY-dependent inhibition of ubiquitylation of the mPER2 clock protein. EMBO J. 21, 1301–1314. doi: 10.1093/emboj/21.6.1301
Yan, J., Wang, H., Liu, Y., and Shao, C. (2008). Analysis of gene regulatory networks in the mammalian circadian rhythm. PLoS Comput. Biol. 4:e1000193. doi: 10.1371/journal.pcbi.1000193
Yang, Y., Duguay, D., Bédard, N., Rachalski, A., Baquiran, G., Na, C. H.,et al. (2012). Regulation of behavioral circadian rhythms and clock protein PER1 by the deubiquitinating enzyme USP2. Biol. Open 1, 789–801. doi: 10.1242/bio.20121990
Yang, Y., Duguay, D., Fahrenkrug, J., Cermakian, N., and Wing, S. S. (2014). USP2 regulates the intracellular localization of PER1 and circadian gene expression. J. Biol. Rhythms (in press).
Yin, L., Joshi, S., Wu, N., Tong, X., and Lazar, M. A. (2010). E3 ligases Arf-bp1 and Pam mediate lithium-stimulated degradation of the circadian heme receptor Rev-erb alpha. Proc. Natl. Acad. Sci. U.S.A. 107, 11614–11619. doi: 10.1073/pnas.1000438107
Yin, L., Wang, J., Klein, P. S., and Lazar, M. A. (2006). Nuclear receptor Rev-erbalpha is a critical lithium-sensitive component of the circadian clock. Science 311, 1002–1005. doi: 10.1126/science.1121613
Yoo, S. H., Mohawk, J. A., Siepka, S. M., Shan, Y., Huh, S. K., Hong, H. K.,et al. (2013). Competing E3 ubiquitin ligases govern circadian periodicity by degradation of CRY in nucleus and cytoplasm. Cell 152, 1091–1105. doi: 10.1016/j.cell.2013.01.055
Yumimoto, K., Muneoka, T., Tsuboi, T., and Nakayama, K. I. (2013). Substrate binding promotes formation of the Skp1-Cul1-Fbxl3 (SCF(Fbxl3)) protein complex. J. Biol. Chem. 288, 32766–32776. doi: 10.1074/jbc.M113.511303
Keywords: circadian clock, clock gene, ubiquitin, ubiquitin ligase, deubiquitinase, stability
Citation: Stojkovic K, Wing SS and Cermakian N (2014) A central role for ubiquitination within a circadian clock protein modification code. Front. Mol. Neurosci. 7:69. doi: 10.3389/fnmol.2014.00069
Received: 14 May 2014; Accepted: 08 July 2014;
Published online: 07 August 2014.
Edited by:
Ashok Hegde, Wake Forest School of Medicine, USAReviewed by:
Jason DeBruyne, Morehouse School of Medicine, USAKazuhiro Yagita, Kyoto Prefectural University of Medicine, Japan
Copyright © 2014 Stojkovic, Wing and Cermakian. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Nicolas Cermakian, Douglas Mental Health University Institute, McGill University, 6875 LaSalle Boulevard, Montréal, QC H4H 1R3, Canada e-mail: nicolas.cermakian@mcgill.ca