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ORIGINAL RESEARCH article

Front. Oncol.
Sec. Pharmacology of Anti-Cancer Drugs
Volume 14 - 2024 | doi: 10.3389/fonc.2024.1321557

Electrochemical DNA-nano biosensor for the detection of Goserelin as anticancer drug using modified pencil graphite electrode

Provisionally accepted
Ahmed J. Jasim Ahmed J. Jasim 1F Al-Dolaimy F Al-Dolaimy 2Salim Albukhaty Salim Albukhaty 3Ghassan M. Sulaiman Ghassan M. Sulaiman 1Ali Alsaalamy Ali Alsaalamy 4Adnan T. Alkhafaji Adnan T. Alkhafaji 5Ali G. Alkhatami Ali G. Alkhatami 6*
  • 1 University of Technology, Iraq, Baghdad, Baghdad, Iraq
  • 2 Al-Zahraa University for Women, Karbala, Karbala, Iraq
  • 3 University of Misan, Amarah, Maysan, Iraq
  • 4 Imam Ja’afar Al‐Sadiq University, Al‐Muthanna, Iraq
  • 5 Al-Ayen University, Nasiriyah, Dhi Qar, Iraq
  • 6 King Khalid University, Abha, Saudi Arabia

The final, formatted version of the article will be published soon.

    Goserelin is an effective anticancer drug, but naturally causes several side effects. Hence the determination of this drug in biological samples, plays a key role in evaluating its effects and side effects. The current studies have concentrated on monitoring Goserelin using an easy and quick DNA biosensor for the first time. In this study, copper(II) oxide nanoparticles were created upon the surface of multiwalled carbon nanotubes (CuO/MWCNTs) as a conducting mediator. The modified pencil graphite electrode (ds-DNA/PA/CuO/MWCNTs/PGE) has been modified with the help of polyaniline (PA), ds-DNA, and CuO/MWCNTs nanocomposite. Additionally, the issue with the bio-electroanalytical guanine oxidation signal in relation to ds-DNA at the surface of PA/CuO/MWCNTs/PGE has been examined to determination Goserelin for the first time. It also, established a strong conductive condition to determination Goserelin in nanomolar concentration.Thus, Goserelin's determining, however, has a 0.21 nM detection limit and a 1.0 nM-110.0 µM linear dynamic range according to differential pulse voltammograms (DPV) of ds-DNA/PA/CuO/MWCNTs/PGE. Furthermore, the molecular docking investigation highlighted that Goserelin is able to bind ds-DNA preferentially and supported the findings of the experiments.The determining of Goserelin in real samples has been effectively accomplished in the last phase using ds-DNA/PA/CuO/MWCNTs/PGE.

    Keywords: Goserelin, Carbon nanotubes, copper oxide, DNA biosensor, Voltammetry

    Received: 08 Dec 2023; Accepted: 22 Mar 2024.

    Copyright: © 2024 Jasim, Al-Dolaimy, Albukhaty, Sulaiman, Alsaalamy, Alkhafaji and Alkhatami. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Ali G. Alkhatami, King Khalid University, Abha, Saudi Arabia

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