Skip to main content

ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Gastrointestinal and Hepatic Pharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1389873
This article is part of the Research Topic Methods in Gastrointestinal and Hepatic Pharmacology 2023 View all 6 articles

Investigating the L-Glu-NMDA receptor-H 2 S-NMDA receptor pathway that regulates gastric function in rats' nucleus ambiguus

Provisionally accepted
Hongzhao Sun Hongzhao Sun *Chenyu Li Chenyu Li Yuan Shi Yuan Shi Yiya Wang Yiya Wang Jinjin Li Jinjin Li Linkun Fan Linkun Fan Yan Yu Yan Yu Xiaofeng Ji Xiaofeng Ji Xiaoting Gao Xiaoting Gao Keyuan Hou Keyuan Hou Yuxue Li Yuxue Li
  • School of Life Science, Qilu Normal University, Jinan, Shandong Province, China

The final, formatted version of the article will be published soon.

    In previous investigations, we explored the regulation of gastric function by hydrogen sulfide (H 2 S) and L-glutamate (L-Glu) injections in the nucleus ambiguus (NA). We also determined that both H 2 S and L-Glu have roles to play in the physiological activities of the body, and that NA is an important nucleus for receiving visceral sensations. The purpose of this study was to explore the potential pathway link between L-Glu and H 2 S, resulting in the regulation of gastric function.: Physiological saline (PS), L-glutamate (L-Glu, 2 nmol), NaHS (2 nmol), D-2-amino-5-phopho-novalerate (D-AP5, 2 nmol) + L-Glu (2 nmol), aminooxyacetic acid (AOAA, 2 nmol) + L-Glu (2 nmol), D-AP5 (2 nmol) + NaHS (2 nmol) were injected into the NA. A balloon was inserted into the stomach to observe gastric pressure and for recording the changes of gastric smooth muscle contraction curve. The gastric fluid was collected by esophageal perfusion and for recording the change of gastric pH value.Results: Injecting L-Glu in NA was found to significantly inhibit gastric motility and promote gastric acid secretion in rats (P < 0.01). On the other hand, injecting the PS, pre-injection Nmethyl-D-aspartate (NMDA) receptor blocker D-AP5, cystathionine beta-synthase (CBS) inhibitor AOAA and re-injection L-Glu did not result in significant changes (P > 0.05). The same injection NaHS significantly inhibit gastric motility and promote gastric acid secretion in rats (P < 0.01), but is eliminated by injection D-AP5 (P > 0.05).The results indicate that both exogenous L-Glu and H 2 S injected in NA regulate gastric motility and gastric acid secretion through NMDA receptors. This suggests that NA has an L-Glu-NMDA receptor-CBS-H 2 S pathway that regulates gastric function.

    Keywords: Nucleus ambiguus, Hydrogen Sulfide, L-Glutamate, gastric motility, Gastric acid secretion

    Received: 22 Feb 2024; Accepted: 15 Apr 2024.

    Copyright: © 2024 Sun, Li, Shi, Wang, Li, Fan, Yu, Ji, Gao, Hou and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Hongzhao Sun, School of Life Science, Qilu Normal University, Jinan, 250200, Shandong Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.