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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Inflammation Pharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1397498
This article is part of the Research Topic Therapeutic Advances in Lung Cancer and Chronic Inflammatory Lung Disease View all 4 articles

Mechanism of isorhynchophylline in lipopolysaccharide-induced acute lung injury based on proteomic technology

Provisionally accepted
Yaru Li Yaru Li 1Junfeng Xing Junfeng Xing 2*Ling Qin Ling Qin 3*Chuanming Zhang Chuanming Zhang 1*Zheng Yang Zheng Yang 3*Min Qiu Min Qiu 1*
  • 1 Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China, Baotou, China
  • 2 Department of Computer Science and Technology，Baotou Medical College, Baotou, Inner Mongolia, China, Baotou, China
  • 3 First Affiliated Hospital of Baotou Medical College,Baotou, China, Baotou, China

The final, formatted version of the article will be published soon.

    Abstract Isorhynchophylline(IRN), a tetracyclic indole alkaloid, has anti-inflammatory and antioxidant activities against cardiovascular diseases and central nervous system disorders. Acute lung injury (ALI) is a manifestation of inflammation concentrated in the lungs and has a high incidence rate and mortality. Here, we established a mouse model of ALI and observed the effects of IRN. Proteomic results showed that 5727 proteins were detected in mouse lung tissues, and 16 proteins were screened out. IRN could reverse the trend of these differential proteins. In addition, IRN can act on integrin αM to reduce neutrophil recruitment and thereby produce anti-inflammatory effects and may suppress neutrophil migration through the leukocyte transendothelial migration pathway. Data are available via ProteomeXchange with identifier PXD050432. TUNEL and RT-PCR experiments revealed that LPS-induced ALI in mice increases the apoptosis of lung tissues, damage to alveolar epithelial cells and levels of inflammatory factors. Treatment with IRN can repair tissues, improve lung tissue pathology and reduce lung inflammation.

    Keywords: TUNEL, RT-PCR, Nano-LC-MS/MS, Isorhynchophylline, Acute Lung Injury, Proteomic technology

    Received: 07 Mar 2024; Accepted: 07 May 2024.

    Copyright: © 2024 Li, Xing, Qin, Zhang, Yang and Qiu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Junfeng Xing, Department of Computer Science and Technology，Baotou Medical College, Baotou, Inner Mongolia, China, Baotou, China
    Ling Qin, First Affiliated Hospital of Baotou Medical College,Baotou, China, Baotou, China
    Chuanming Zhang, Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China, Baotou, China
    Zheng Yang, First Affiliated Hospital of Baotou Medical College,Baotou, China, Baotou, China
    Min Qiu, Department of Pharmacy, Baotou Medical College, Baotou, Inner Mongolia, China, Baotou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.