Original Research ARTICLE
Further insights into the interaction of human and animal complement regulator factor H with viable Lyme disease spirochetes
- 1Universitätsklinikum Frankfurt, Germany
- 2New York State Department of Health, United States
Spirochetes belonging to the Borrelia (B.) burgdorferi sensu lato (s.l.) complex differ in their ability to establish infection and to survive in diverse vertebrate hosts. Association with and adaption to various hosts most likely correlates with the spirochetes’ ability to acquire complement regulator factor H (FH) to overcome the host´s innate immune response. Here we assessed binding of serum FH from human and various animals including bovine, cat, chicken, dog, horse, mouse, rabbit, and rat to viable B. burgdorferi sensu stricto (s.s.), B. afzelii, B. garinii, B. spielmanii, B. valaisiana, and B. lusitaniae. Spirochetes ectopically producing CspA orthologs of B. burgdorferi s.s., B. afzelii, and B. spielmanii, CspZ, ErpC, and ErpP, respectively, were also investigated. Our comparative analysis using viable bacterial cells revealed a striking heterogeneity among Lyme disease spirochetes regarding their FH-binding patterns that almost mirrors the serum susceptibility of the respective borrelial genospecies. Moreover, native CspA from B. burgdorferi s.s., B. afzelii, and B. spielmanii as well as CspZ were identified as key ligands of FH from human, horse, and rat origin while ErpP appears to bind dog and mouse FH and to a lesser extent human FH. By contrast, ErpC did not bind FH from human as well as from animal origin. These findings indicate a strong restriction of distinct borrelial proteins towards binding of polymorphic FH of various vertebrate hosts.
Keywords: Borrelia, Complement - immunological terms, Factor H (FH), Lyme Disease, Immune Evasion, Spirochete bacteria, Host selectivity
Received: 08 Oct 2018;
Accepted: 28 Dec 2018.
Edited by:Artur Summerfield, Institute of Virology and Immunology (IVI), Switzerland
Reviewed by:Jo Stevens, University of Edinburgh, United Kingdom
Juan Mosqueda, Universidad Autónoma de Querétaro, Mexico
Copyright: © 2018 Mühleip, Lin and Kraiczy. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. Peter Kraiczy, Universitätsklinikum Frankfurt, Frankfurt, 60590, Hesse, Germany, Kraiczy@em.uni-frankfurt.de