Ginseng intake and Alzheimer disease-specific cognition in older adults according to apolipoprotein ε4 allele status

Abstract

Background:

The probable association among ginseng intake, Alzheimer’s disease (AD)-specific cognition, and apolipoprotein ε4 (APOE4) remains poorly investigated. Hence, we examined the association between ginseng intake and AD-specific cognition in older adults under the moderating effect of APOE4 status.

Methods:

This study enrolled 160 adults aged 65–90 years without dementia. All participants underwent comprehensive dietary and clinical assessments including ginseng intake, AD-related cognition (i.e., delayed episodic memory, as the earliest cognitive change in AD), and non-memory cognition for comparative purposes.

Results:

Ginseng intake was associated with higher delayed episodic memory, but not non-memory cognition, compared to no ginseng intake. The interaction between ginseng intake and APOE4 status had a significant effect on delayed episodic memory. Subgroup analyses showed that ginseng intake was associated with higher delayed episodic memory in the APOE4-negative but not the APOE4-positive subgroup. The benefits of ginseng intake on delayed episodic memory were prominent in the high duration (≥5 years) and midlife onset (<65 years) groups.

Conclusion:

Our study of older adults with no dementia suggests that ginseng intake (with high duration and midlife onset) had a beneficial effect on AD-specific cognitive decline, i.e., the delayed episodic memory. In addition, APOE4 status moderates the association between ginseng intake status and AD-specific cognitive decline.

Introduction

Alzheimer’s disease (AD) is a chronic neurodegenerative disease that primarily affects memory-related cognitive performance in the elderly (Querfurth and LaFerla, 2010). The earliest cognitive change in AD is episodic memory decline, particularly in delayed episodic memory decline (Howieson et al., 1997; Grober et al., 2000; Laakso et al., 2000; Bäckman et al., 2001, 2005; Tromp et al., 2015). AD is one of the most detrimental diseases worldwide because no therapeutic drug is currently available. Apolipoprotein ε4 (APOE4), the highest-risk gene for AD (Yamazaki et al., 2019), affects the pathway serves to increase Aβ pathology in the brains of APOE4-positive older adults (Morris et al., 2010; Yamazaki et al., 2019), and it may reduce function in multiple brain homeostatic pathways thereby causing cognitive impairment and dementia (Lowe et al., 2014; Yamazaki et al., 2019; Jeon et al., 2020).

In this background, ginseng, a widely used traditional medicine for centuries, could be a new alternative in the treatment of AD. Ginseng has been reported to have protective effects via multiple brain pathways, including anti-oxidative, anti-inflammatory, and immunomodulatory properties (Park et al., 2013; Hossen et al., 2017), and some preclinical studies have revealed that ginseng has an inhibitory effect on Aβ pathology (Lee et al., 2001; Kim et al., 2013; Choi et al., 2017, 2020). Many human studies have also reported non-specific cognitive benefits of ginseng (Geng et al., 2010; Lho et al., 2018). However, few human studies have investigated the effect of ginseng on the AD-specific cognitive domain, i.e., delayed episodic memory (Howieson et al., 1997; Grober et al., 2000; Laakso et al., 2000; Bäckman et al., 2001, 2005; Tromp et al., 2015). In addition, it is probable that ginseng affects AD and cognitive dysfunction based on an individual’s APOE4 status, as APOE4 is the most important gene in AD (Yamazaki et al., 2019). However, some aspects of AD and the associated cognitive decline, including the interaction between ginseng and APOE4, remain unclear.

Therefore, we first investigated the association between ginseng and AD-specific cognition, i.e., delayed episodic memory decline, in non-demented older adults. We then examined the moderating effect of APOE4 status on this association and the association between ginseng and non-memory cognition for comparative purposes.

Methods

Participants

The present study enrolled 160 non-demented older adults (age 65 to 90 years); 93 cognitively normal (CN) adults and 67 with mild cognitive impairment (MCI). Subjects recruited in the study are adults who attended a dementia screening program offered by the memory clinic of Hallym University Dongtan Sacred Heart Hospital, Hwaseong, Republic of Korea. Volunteers were invited for an eligibility assessment. Additional volunteers were enrolled from the community through recommendations from other subjects, acquaintances, friends, or family members.

The CN group comprised subjects with a Clinical Dementia Rating (Morris, 1993) score of 0 and no diagnosis of MCI or dementia. All MCI subjects met the current consensus for amnestic MCI criteria, including memory complaints confirmed by an informant, objective memory problem, preservation of global cognitive performance, independence in functional activities, and the absence of dementia. In the objective memory impairment assessment, the age-, education-, and sex-adjusted z-score was < −1.0 on at least one of 4 episodic memory tests in the Korean version of the Consortium to Establish a Registry for Alzheimer’s Disease (CERAD) neuropsychological battery (Morris et al., 1989; Lee et al., 2002): Word List Memory (WLM), Word List Recall (WLR), Word List Recognition (WLRc), and the Constructional Recall (CR) test (Morris et al., 1989; Lee et al., 2002, 2004). All MCI subjects had a Clinical Dementia Rating score of 0.5. The exclusion criteria were the presence of a major psychiatric disorders, a significant neurological or medical illness, or a comorbidity that could affect cognitive functioning; illiteracy; visual/hearing difficulties or severe communication or behavioral problems that could make clinical examinations difficult; and the use of an investigational drug. The study protocol was approved by the Institutional Review Board of the Hallym University Dongtan Sacred Heart Hospital. The study was conducted it in accordance with the recommendations of the current version of the Declaration of Helsinki and all subjects have provided informed consent.

Clinical assessments

All subjects underwent standardized clinical assessments by psychiatrists with more than 10 years of clinical experience based on the clinical assessment protocol, which incorporates the CERAD clinical and neuropsychological battery (Morris et al., 1989; Lee et al., 2002). Trained psychometrists administered the neuropsychological assessment protocol incorporating the CERAD neuropsychological battery (Lee et al., 2004) to all subjects. Memory (immediate or delayed episodic memory) and non-memory cognition scores were also measured. The episodic memory score (EMS) was determined by summing the scores of 4 episodic memory tests (WLM, WLR, WLRc, and CR). Immediate EMS was determined by the score of WLM. Delayed EMS, as the earliest cognitive change in AD (Howieson et al., 1997; Grober et al., 2000; Laakso et al., 2000; Bäckman et al., 2001, 2005; Tromp et al., 2015), was determined by summing the scores of 3 delayed episodic memory tests (WLR, WLRc, and CR). The non-memory score (NMS) was calculated by summing the scores of 3 non-memory tests (verbal fluency, modified Boston naming test, and constructional praxis). Vascular risks (e.g., hypertension, diabetes mellitus, dyslipidemia, coronary heart disease, transient ischemic attack, and stroke) were measured based on data collected by trained psychometrists during systematic interviews of the subjects and their families. The vascular risk score (VRS) was computed based on the number of vascular risk factors present and was reported as a percentage (DeCarli et al., 2004). The Geriatric Depression Scale (GDS) was used to assess the severity of depressive symptoms (Yesavage et al., 1982; Kim et al., 2008). Lifetime alcohol intake status (never/former/drinker) were evaluated by trained psychometrists interviews and a medical record review. The accuracy of the information was ensured by interviewing reliable informants.

Assessment of ginseng intake and other dietary patterns

All subjects were assessed systematically to identify their ginseng intake patterns, i.e., ginseng type (white-or red-ginseng in any formula containing ginseng extract), the age at initial ginseng intake, and the frequency of ginseng intake (number of days per month).(Lho et al., 2018) As the quantity of ginseng varies between ginseng items and there is a low probability of obtaining the exact quantity of ginseng in any given item, we defined ‘ginseng intake’ by subtracting the age at initial ginseng intake from the age at study entry, adding 1 year to reflect the intake of the first year, and then multiplying the result by a year’s intake, calculated by multiplying the number of days of ginseng intake in a month by 12 months (Lho et al., 2018). As the median of the years of cumulative ginseng intake in subjects with ginseng consumption was 5 years, ginseng intake was categorized as ‘no use,’ ‘low use’ (< 5 years), or ‘high use’ (≥ 5 years).(Lho et al., 2018) We also assessed dietary patterns including food types, such as protein, fruit or vegetables, using the items from the mini nutritional assessment (MNA) tools, which is a brief, valid nutritional evaluation tool for elderly populations (Vellas et al., 1999).

Blood test

Blood samples were obtained by venipuncture in the morning after an overnight fast. Albumin, glucose, and high-(HDL) and low-density lipoprotein (LDL)-cholesterol were measured using a Roche COBAS c702 analyzer and dedicated reagents (Roche Diagnostics, Mannheim, Germany).

APOE genotyping

Blood samples were collected into vacutainer tubes with EDTA anticoagulant. DNA was extracted using QIAamp DSP DNA Blood mini kits (QIAGEN, Hilden, Germany) and the QIAcube HT System (QIAGEN). APOE genotyping was performed with the aid of a Seeplex ApoE ACE Genotyping Kit (Seegene, Seoul, Korea) and the ProFlex PCR system (ThermoFisher Scientific, Waltham, MA, USA) according to the manufacturers’ instructions. PCR products were analyzed via capillary electrophoresis (QIAxcel Advanced System, QIAGEN) and labeled ε2/ε2, ε2/ε3, ε2/ε4, ε3/ε3, ε3/ε4, or ε4/ε4 by the electrophoresis patterns as instructed by the manufacturer. APOE4-positivity was defined as the presence of at least one ε4 allele.

Statistical analysis

We first compared the demographic and clinical characteristics by ginseng intake status (no intake vs. ginseng intake) using the Student t-test to analyze continuous data and the chi-square test or Fisher exact test to evaluate categorical data. To examine the relationship between ginseng intake and cognition, multiple linear regression analyses were performed with ginseng intake as the independent variable and cognition as the dependent variable. For these analyses, we used the “no ginseng intake” as the reference. As various factors may influence the association between ginseng intake and cognition, we systematically evaluated all subjects to identify potential confounders, such as vascular risks, depression, annual income, alcohol intake, smoking, blood nutritional markers (e.g., albumin, glucose, or HDL-and LDL-cholesterol), and dietary patterns including food types (e.g., protein, fruits, or vegetables). We tested two models, adjusting for the covariates in a stepwise manner. The first model included age, sex, APOE4 status, education, clinical diagnosis, VRS, GDS, and blood nutritional markers (e.g., albumin, glucose, or HDL-and LDL-cholesterol) as covariates; the second model included those covariates plus annual income status, alcohol intake, smoking and dietary patterns including food types (e.g., protein, fruits, or vegetables).

The moderating effect of APOE4 status on the association between ginseng intake and cognition was examined in multiple linear regression analyses including a two-way interaction terms for the association between ginseng intake and cognition as additional independent variables. When an interaction was determined, linear regression analyses were repeated individually depending on whether the APOE4 was positive or negative. To examine differential effects, the similar sensitivity analyses were performed using only the subjects with cognitive impairment because the cognitive score may be less discriminatory in CN individuals. In addition, the similar sensitivity analyses were performed using the subjects without decrease in food intake over the past 3 months. In order to eliminate possible effects of physical or mental conditions in relation to both ginseng intake or cognition, subjects with changes of food intake due to loss of appetite, digestive problems, and chewing or swallowing difficulties were excluded from the analyses. All statistical analyses were performed using SPSS Statistics ver. 28 (IBM Corp, Armonk, NY, United States).

Results

Participant characteristics

Table 1 summarizes the demographic and clinical characteristics of the 160 participants by ginseng intake status (80 who took ginseng and the 80 who did not). None of the study subjects were malnourished, i.e., serum albumin <3.5 g/dL (Cabrerizo et al., 2015).

Association between ginseng intake and cognition

As shown in Table 2 and Figure 1, ginseng intake was associated with higher AD-specific cognition, i.e., delayed EMS compared to no ginseng intake, regardless of the model tested. By contrast, we did not find any significant difference in other cognition, i.e., immediate EMS and NMS between the ginseng intake groups.

Figure 1

APOE4 status moderation of the association between ginseng intake and cognition

As shown in Table 3, the interaction between the ginseng intake and APOE4 status had a significant effect on the delayed EMS but neither the immediate EMS nor NMS. This indicates that APOE4 status has a moderating effect on the associations between the ginseng intake and delayed episodic memory cognition, but neither immediate episodic memory nor the NMS. As shown in Table 4, subgroup analyses according to APOE4 status showed that the ginseng intake was associated with a higher delayed EMS in the APOE4-negative but not the APOE4-positive subgroup.

The interaction effect of ginseng intake and APOE4 status on delayed EMS was statistically significant (B = −2.241, p < 0.001), indicating that the association between ginseng intake and delayed EMS varied depending on APOE4 status. Specifically, the difference in delayed EMS associated with ginseng intake was significantly greater in the APOE4 negative group than in the APOE4 positive group. The negative B value reflects the direction of the interaction effect, where the relationship between ginseng intake and delayed EMS is positive (B = 3.244) in the APOE4 negative group but significantly less positive (B = 2.327) in the APOE4 positive group.

Association between ginseng intake with (high vs. low) duration or (midlife vs. late life) onset and cognition

As shown in Tables 5, 6, ginseng intake was associated with higher delayed EMS in the high duration (≥ 5 years) and midlife onset (< 65 years) groups.

Sensitivity analyses

The results of the sensitivity analysis of subjects with MCI and those without a decrease in food intake over the past 3 months were similar for the delayed EMS, immediate EMS, and NMS (Table 7).

Discussion

This study of non-demented older adults found that ginseng intake had a benefit on AD-specific cognition, i.e., the delayed episodic memory. It also showed that APOE4 status had a moderating effect on the association between ginseng intake status and delayed episodic memory cognition, and that ginseng intake had a benefit on the delayed episodic memory cognition in APOE4-negative but not APOE4-positive subjects. This may reflect interactions among ginseng intake, APOE4 status, and AD or related cognitive decline.

This study found that the benefit of ginseng intake was observed mainly on memory, not on non-memory, and it was more prominent in delayed episodic memory than immediate episodic memory. The earliest cognitive change in patients who will develop AD is episodic memory decline, particularly in delayed episodic memory decline (Howieson et al., 1997; Grober et al., 2000; Laakso et al., 2000; Bäckman et al., 2001, 2005; Tromp et al., 2015). Therefore, our results suggest that ginseng intake acts mostly on delayed episodic memory decline and may play a clinically significant role in early AD-related cognitive decline.

Previously, neuroprotective effects of ginseng on the pathological and clinical process of AD was reported, which may explain the mechanism underlying the relationship between ginseng intake and early AD-related cognitive decline (Razgonova et al., 2019; Li et al., 2021; Wang and Liu, 2023). In cell and animal models, ginseng acted on AD by regulating multiple signaling pathways, such as, the phosphatidylinositol 3-kinase/protein kinase B, adenosine-monophosphate activated-protein kinase/mammalian target of rapamycin, and nuclear factor kappa B pathways, in order to block or improve pathological processes, such as Aβ accumulation, tau phosphorylation, neuroinflammation, neurotrophic factors, apoptosis, and mitochondrial dysfunction in AD (Razgonova et al., 2019; Li et al., 2021; Wang and Liu, 2023). One preclinical study using an AD model revealed that ginseng treatment significantly improved learning and memory performance at 14 months of age and reduced brain senile plaques at this age (Qiu et al., 2014). In vitro experiments also found that ginseng treatment reduced Aβ 40 and 42 concentrations (Qiu et al., 2014). Another preclinical study in a transgenic mouse AD model found that long-term ginseng treatment attenuated Aβ deposition and short-and long-term memory impairment (Hwang et al., 2012). Clinical trials found that ginseng significantly improved working memory and attention in healthy young adults (Bell et al., 2022), global cognition and executive function in subjects with subjective memory impairment (Lee et al., 2022), visual memory function in MCI patients (Park et al., 2019), and global cognition in AD patients (Lee et al., 2008; Heo et al., 2012). Based on the preclinical findings, ginseng improves cognitive features of AD patients via AD pathologies, i.e., Aβ accumulation and tau phosphorylation. In human, however, the precise mechanism of ginseng in the pathologies of AD in the prevention and treatment of AD remain unclear.

The present study found that APOE4 status modulated the relationship between ginseng intake and delayed episodic memory cognition. A significant association between ginseng intake and delayed episodic memory cognition was apparent in subjects without APOE4, but not those with APOE4. We hypothesize that the benefit of ginseng may be masked in the latter subjects because APOE4 triggers the blood–brain barrier (BBB) dysfunction that predicts cognitive decline (Montagne et al., 2020). Also, Aβ clearance may differ by the APOE isoform, and could thus be affected by ginseng intake. APOE4 stimulates Aβ clearance less effectively than do APOE3 and APOE2; APOE4 status (compared to non-APOE4 status) may significantly inhibit Aβ removal (Wildsmith et al., 2013). Therefore, the protective effect of ginseng on AD-related cognition is apparent only in the APOE4-negative group because the benefit of ginseng is offset by the APOE4 affects the shared Aβ clearance pathway (Kurz and Perneczky, 2011).

The present study also found that ginseng intake was associated with a higher delayed EMS in the high duration (≥ 5 years) and midlife onset (< 65 years) groups. Regarding the high duration effect of ginseng on delayed EMS, two studies were similar to ours. A randomized open-label study indicated that long-term ginseng treatment for up to 2 years improved the cognitive deficit in AD patients (Heo et al., 2011). A population-based prospective cohort study of older Koreans also revealed that the high-use group (≥ 5 years) had a higher global cognitive score than the no-use group, which means that ginseng use for longer than 5 years may benefit cognitive function in late life (Lho et al., 2018). Regarding the effect of ginseng intake with midlife onset on delayed EMS, a human study supported our finding of a highly significant association between increasing age and decreased Aβ turnover rates, i.e., 2.5-fold longer half-life over five decades of age (Patterson et al., 2015). These changes in Aβ kinetics associated with aging present opportunities for treatment strategies, such as ginseng as an early intervention in middle age rather than old age.

Limitations

Our study had several limitations. First, as it was cross-sectional in its design, causal relationships could not be inferred, which would require long-term prospective studies. Second, it did not measure brain AD pathology biomarkers, e.g., Aβ, tau, and APOE4-related metabolic biomarkers. These should be analyzed in future studies of the link between the ginseng intake and AD or related cognitive decline in APOE-negative older adults. Third, there was a possibility of recall biases in the ginseng intake history. Therefore, we evaluated the ginseng intake history in non-demented older adults using intensive interviews by researchers, not a questionnaire. Furthermore, information accuracy was ensured by interviewing reliable informants. Finally, the association between ginseng intake and AD or related cognitive decline may vary by ginseng dose. Two clinical trials assessed the dose-effects of ginseng on physical (Lee et al., 2018) and cognitive (Kennedy et al., 2001) performances; high-dose ginseng improved performance. However, in both studies, young (not older) adults received ginseng short-term. Ginseng efficacy may be affected by all of dose, administration period, and subject age. However, we could not measure the ginseng doses because commercial products do not consistently or clearly indicate the active ginseng levels. Long-term studies delivering defined ginseng doses to older adults are needed.

Conclusion

Our study of older adults with no dementia suggests that ginseng intake (with high duration and midlife onset) had a benefit on AD-specific cognitive decline, i.e., the delayed episodic memory. In addition, APOE4 status moderates the association between ginseng intake status and AD-specific cognitive decline. Thus, APOE4 status could be considered a key component in the design of studies of AD and related cognitive decline that include ginseng intake as a preventive strategy.

Funding

This study was supported by the grants from the 2021 Korean Society of Ginseng. The funding sources had no role in the study design, data collection, data analysis, data interpretation, writing of the manuscript, or decision to submit it for publication.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

References

• 1

  BäckmanL.JonesS.BergerA. K.LaukkaE. J.SmallB. J. (2005). Cognitive impairment in preclinical Alzheimer's disease: a meta-analysis. Neuropsychology19, 520–531. doi: 10.1037/0894-4105.19.4.520

  • CrossRef
  • Google Scholar
  • View reference in article

• 2

  BäckmanL.SmallB. J.FratiglioniL. (2001). Stability of the preclinical episodic memory deficit in Alzheimer's disease. Brain124, 96–102. doi: 10.1093/brain/124.1.96

  • CrossRef
  • Google Scholar
  • View reference in article

• 3

  BellL.WhyteA.DuysburghC.MarzoratiM.van den AbbeeleP.le CozannetR.et al. (2022). A randomized, placebo-controlled trial investigating the acute and chronic benefits of American ginseng (Cereboost(R)) on mood and cognition in healthy young adults, including in vitro investigation of gut microbiota changes as a possible mechanism of action. Eur. J. Nutr.61, 413–428. doi: 10.1007/s00394-021-02654-5

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 4

  CabrerizoS.CuadrasD.Gomez-BustoF.Artaza-ArtabeI.Marín-CiancasF.MalafarinaV. (2015). Serum albumin and health in older people: review and meta analysis. Maturitas81, 17–27. doi: 10.1016/j.maturitas.2015.02.009

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 5

  ChoiJ. G.KimN.HuhE.LeeH.OhM. H.ParkJ. D.et al. (2017). White ginseng protects mouse hippocampal cells against amyloid-Beta oligomer toxicity. Phytother. Res.31, 497–506. doi: 10.1002/ptr.5776

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 6

  ChoiS.LimJ. W.KimH. (2020). Korean red ginseng inhibits amyloid-beta-induced apoptosis and Nucling expression in human neuronal cells. Pharmacology105, 586–597. doi: 10.1159/000505592

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 7

  DeCarliC.MungasD.HarveyD.ReedB.WeinerM.ChuiH.et al. (2004). Memory impairment, but not cerebrovascular disease, predicts progression of MCI to dementia. Neurology63, 220–227. doi: 10.1212/01.WNL.0000130531.90205.EF

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 8

  GengJ.DongJ.NiH.LeeM. S.WuT.JiangK.et al. (2010). Ginseng for cognition. Cochrane Database Syst. Rev.12:CD007769. doi: 10.1002/14651858.CD007769

  • CrossRef
  • Google Scholar
  • View reference in article

• 9

  GroberE.LiptonR. B.HallC.CrystalH. (2000). Memory impairment on free and cued selective reminding predicts dementia. Neurology54, 827–832. doi: 10.1212/WNL.54.4.827

  • CrossRef
  • Google Scholar
  • View reference in article

• 10

  HeoJ. H.LeeS. T.ChuK.OhM. J.ParkH. J.ShimJ. Y.et al. (2012). Heat-processed ginseng enhances the cognitive function in patients with moderately severe Alzheimer's disease. Nutr. Neurosci.15, 278–282. doi: 10.1179/1476830512Y.0000000027

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 11

  HeoJ. H.LeeS. T.OhM. J.ParkH. J.ShimJ. Y.ChuK.et al. (2011). Improvement of cognitive deficit in Alzheimer's disease patients by long term treatment with korean red ginseng. J. Ginseng Res.35, 457–461. doi: 10.5142/jgr.2011.35.4.457

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 12

  HossenM. J.HongY. D.BaekK. S.YooS.HongY. H.KimJ. H.et al. (2017). In vitro antioxidative and anti-inflammatory effects of the compound K-rich fraction BIOGF1K, prepared from Panax ginseng. J. Ginseng Res.41, 43–51. doi: 10.1016/j.jgr.2015.12.009

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 13

  HowiesonD. B.DameA.CamicioliR.SextonG.PayamiH.KayeJ. A. (1997). Cognitive markers preceding Alzheimer's dementia in the healthy oldest old. J. Am. Geriatr. Soc.45, 584–589. doi: 10.1111/j.1532-5415.1997.tb03091.x

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 14

  HwangS. H.ShinE. J.ShinT. J.LeeB. H.ChoiS. H.KangJ.et al. (2012). Gintonin, a ginseng-derived lysophosphatidic acid receptor ligand, attenuates Alzheimer's disease-related neuropathies: involvement of non-amyloidogenic processing. J. Alzheimers Dis.31, 207–223. doi: 10.3233/JAD-2012-120439

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 15

  JeonS. Y.ByunM. S.YiD.LeeJ. H.KoK.SohnB. K.et al. (2020). Midlife lifestyle activities moderate APOE epsilon 4 effect on in vivo Alzheimer's disease pathologies. Front. Aging Neurosci.12:42. doi: 10.3389/fnagi.2020.00042

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 16

  KennedyD. O.ScholeyA. B.WesnesK. A. (2001). Differential, dose dependent changes in cognitive performance following acute administration of a Ginkgo biloba/Panax ginseng combination to healthy young volunteers. Nutr. Neurosci.4, 399–412. doi: 10.1080/1028415X.2001.11747376

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 17

  KimJ.KimS. H.LeeD. S.LeeD. J.KimS. H.ChungS.et al. (2013). Effects of fermented ginseng on memory impairment and beta-amyloid reduction in Alzheimer's disease experimental models. J. Ginseng Res.37, 100–107. doi: 10.5142/jgr.2013.37.100

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 18

  KimJ. Y.ParkJ. H.LeeJ. J.HuhY.LeeS. B.HanS. K.et al. (2008). Standardization of the korean version of the geriatric depression scale: reliability, validity, and factor structure. Psychiatry Investig.5, 232–238. doi: 10.4306/pi.2008.5.4.232

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 19

  KurzA.PerneczkyR. (2011). Amyloid clearance as a treatment target against Alzheimer's disease. J. Alzheimers Dis.24, 61–73. doi: 10.3233/JAD-2011-102139

  • CrossRef
  • Google Scholar
  • View reference in article

• 20

  LaaksoM. P.HallikainenM.HänninenT.PartanenK.SoininenH. (2000). Diagnosis of Alzheimer's disease: MRI of the hippocampus vs delayed recall. Neuropsychologia38, 579–584. doi: 10.1016/S0028-3932(99)00111-6

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 21

  LeeD. Y.LeeK. U.LeeJ. H.KimK. W.JhooJ. H.KimS. Y.et al. (2004). A normative study of the CERAD neuropsychological assessment battery in the Korean elderly. J. Int. Neuropsychol. Soc.10, 72–81. doi: 10.1017/S1355617704101094

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 22

  LeeE. S.YangY. J.LeeJ. H.YoonY. S. (2018). Effect of high-dose ginsenoside complex (UG 0712) supplementation on physical performance of healthy adults during a 12-week supervised exercise program: a randomized placebo-controlled clinical trial. J. Ginseng Res.42, 192–198. doi: 10.1016/j.jgr.2017.03.001

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 23

  LeeJ. H.LeeK. U.LeeD. Y.KimK. W.JhooJ. H.KimJ. H.et al. (2002). Development of the Korean version of the consortium to establish a registry for Alzheimer's disease assessment packet (CERAD-K): clinical and neuropsychological assessment batteries. J. Gerontol. B Psychol. Sci. Soc. Sci.57, 47–53. doi: 10.1093/geronb/57.1

  • CrossRef
  • Google Scholar
  • View reference in article

• 24

  LeeS. T.ChuK.SimJ. Y.HeoJ. H.KimM. (2008). Panax ginseng enhances cognitive performance in Alzheimer disease. Alzheimer Dis. Assoc. Disord.22, 222–226. doi: 10.1097/WAD.0b013e31816c92e6

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 25

  LeeT. F.ShiaoY. J.ChenC. F.WangL. C. H. (2001). Effect of ginseng saponins on beta-amyloid-suppressed acetylcholine release from rat hippocampal slices. Planta Med.67, 634–637. doi: 10.1055/s-2001-17366

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 26

  LeeW. J.ShinY. W.ChangH.ShinH. R.KimW. W.JungS. W.et al. (2022). Safety and efficacy of dietary supplement (gintonin-enriched fraction from ginseng) in subjective memory impairment: a randomized placebo-controlled trial. Integr Med Res11:100773. doi: 10.1016/j.imr.2021.100773

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 27

  LhoS. K.KimT. H.KwakK. P.KimK.KimB. J.KimS. G.et al. (2018). Effects of lifetime cumulative ginseng intake on cognitive function in late life. Alzheimers Res. Ther.10:50. doi: 10.1186/s13195-018-0380-0

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 28

  LiJ.HuangQ.ChenJ.QiH.LiuJ.ChenZ.et al. (2021). Neuroprotective potentials of Panax Ginseng against Alzheimer's disease: a review of preclinical and clinical evidences. Front. Pharmacol.12:688490. doi: 10.3389/fphar.2021.688490

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 29

  LoweV. J.WeigandS. D.SenjemM. L.VemuriP.JordanL.KantarciK.et al. (2014). Association of hypometabolism and amyloid levels in aging, normal subjects. Neurology82, 1959–1967. doi: 10.1212/WNL.0000000000000467

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 30

  MontagneA.NationD. A.SagareA. P.BarisanoG.SweeneyM. D.ChakhoyanA.et al. (2020). APOE4 leads to blood-brain barrier dysfunction predicting cognitive decline. Nature581, 71–76. doi: 10.1038/s41586-020-2247-3

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 31

  MorrisJ. C. (1993). The clinical dementia rating (CDR): current version and scoring rules. Neurology43, 2412–2414.

  • Google Scholar
  • View reference in article

• 32

  MorrisJ. C.HeymanA.MohsR. C.HughesJ. P.van BelleG.FillenbaumG.et al. (1989). The consortium to establish a registry for Alzheimer's disease (CERAD). Part I. clinical and neuropsychological assessment of Alzheimer's disease. Neurology39, 1159–1165. doi: 10.1212/WNL.39.9.1159

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 33

  MorrisJ. C.RoeC. M.XiongC.FaganA. M.GoateA. M.HoltzmanD. M.et al. (2010). APOE predicts amyloid-beta but not tau Alzheimer pathology in cognitively normal aging. Ann. Neurol.67, 122–131. doi: 10.1002/ana.21843

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 34

  ParkB. G.JungH. J.ChoY. W.LimH. W.LimC. J. (2013). Potentiation of antioxidative and anti-inflammatory properties of cultured wild ginseng root extract through probiotic fermentation. J. Pharm. Pharmacol.65, 457–464. doi: 10.1111/jphp.12004

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 35

  ParkK. C.JinH.ZhengR.KimS.LeeS. E.KimB. H.et al. (2019). Cognition enhancing effect of panax ginseng in Korean volunteers with mild cognitive impairment: a randomized, double-blind, placebo-controlled clinical trial. Transl. Clin. Pharmacol.27, 92–97. doi: 10.12793/tcp.2019.27.3.92

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 36

  PattersonB. W.ElbertD. L.MawuenyegaK. G.KastenT.OvodV.MaS.et al. (2015). Age and amyloid effects on human central nervous system amyloid-beta kinetics. Ann. Neurol.78, 439–453. doi: 10.1002/ana.24454

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 37

  QiuJ.LiW.FengS. H.WangM.HeZ. Y. (2014). Ginsenoside Rh2 promotes nonamyloidgenic cleavage of amyloid precursor protein via a cholesterol-dependent pathway. Genet. Mol. Res.13, 3586–3598. doi: 10.4238/2014.May.9.2

  • Pubmed Abstract
  • CrossRef
  • Google Scholar
  • View reference in article

• 38

  QuerfurthH. W.LaFerlaF. M. (2010). Alzheimer's disease. N. Engl. J. Med.362, 329–344. doi: 10.1056/NEJMra0909142

  • CrossRef
  • Google Scholar

• 39

  RazgonovaM. P.VeselovV. V.ZakharenkoA. M.GolokhvastK. S.NosyrevA. E.CravottoG.et al. (2019). Panax ginseng components and the pathogenesis of Alzheimer's disease (review). Mol. Med. Rep.19, 2975–2998. doi: 10.3892/mmr.2019.9972

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 40

  TrompD.DufourA.LithfousS.PebayleT.DesprésO. (2015). Episodic memory in normal aging and Alzheimer disease: insights from imaging and behavioral studies. Ageing Res. Rev.24, 232–262. doi: 10.1016/j.arr.2015.08.006

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 41

  VellasB.GuigozY.GarryP. J.NourhashemiF.BennahumD.LauqueS.et al. (1999). The mini nutritional assessment (MNA) and its use in grading the nutritional state of elderly patients. Nutrition15, 116–122. doi: 10.1016/S0899-9007(98)00171-3

  • CrossRef
  • Google Scholar

• 42

  WangY.LiuX. (2023). The effective components, Core targets, and key pathways of ginseng against Alzheimer's disease. Evid. Based Complement. Alternat. Med.2023:9935942. doi: 10.1155/2023/9935942

  • CrossRef
  • Google Scholar

• 43

  WildsmithK. R.HolleyM.SavageJ. C.SkerrettR.LandrethG. E. (2013). Evidence for impaired amyloid beta clearance in Alzheimer's disease. Alzheimers Res. Ther.5:33. doi: 10.1186/alzrt187

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 44

  YamazakiY.ZhaoN.CaulfieldT. R.LiuC. C.BuG. (2019). Apolipoprotein E and Alzheimer disease: pathobiology and targeting strategies. Nat. Rev. Neurol.15, 501–518. doi: 10.1038/s41582-019-0228-7

  • Pubmed Abstract
  • CrossRef
  • Google Scholar

• 45

  YesavageJ. A.BrinkT. L.RoseT. L.LumO.HuangV.AdeyM.et al. (1982). Development and validation of a geriatric depression screening scale: a preliminary report. J. Psychiatr. Res.17, 37–49. doi: 10.1016/0022-3956(82)90033-4

  • Pubmed Abstract
  • CrossRef
  • Google Scholar