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ORIGINAL RESEARCH article

Front. Aging

Sec. Interventions in Aging

Anshen Bunao syrup as a potential anti-aging agent: mechanistic insights and pharmacological evidence

Provisionally accepted
YuanFang SunYuanFang Sun1Qi XiaQi Xia2Ruolan WuRuolan Wu2Gang LiuGang Liu3Yongkuan WangYongkuan Wang3Shikai YanShikai Yan1Huizi JinHuizi Jin1*Xiuyun ZhangXiuyun Zhang3*XUE XIAOXUE XIAO4*Shasha LiShasha Li2
  • 1Shanghai Jiao Tong University, Shanghai, China
  • 2The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
  • 3Jilin Aodong Yanbian Pharmaceutical Co., Ltd., Dunhua, China
  • 4Guangdong Pharmaceutical University, Guangzhou, China

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Background: Anshen Bunao Syrup (ABS) is a commonly used traditional Chinese patent medicine for insomnia and amnesia, and also has the potential to anti-aging but lacks scientific support of pharmacological and mechanism research. This study aims to investigate the anti-aging effect of ABS and its underlying mechanism. Methods: Chemical constituents of ABS were analyzed by LC/MS. ABS effects were assessed on d-galactose induced aging rat model through open field tests, histopathological examinations, senescence-associated secretory phenotypes and biochemical assay. Integrated analysis of transcriptomics and metabolomics was conducted to identify differentially expressed genes and metabolites and to elucidate the potential mechanism. Results: 159 chemical constituents in ABS were profiled and structurally presumed. ABS can significantly alleviate skin aging, neural damage and other aging related symptoms, and as well can reduce the expression of senescence-associated secretory phenotypes. Integrated transcriptomics and metabolomics revealed that ABS treatment modulates the expression levels of CYP2J10, CBR3, and LPAR3, and restores sphingolipid and arachidonic acid metabolism. ELISA results indicated that ABS reduces pro-inflammatory factor levels and relieve oxidative stress. Conclusions: ABS exerts anti-aging effects by regulating sphingolipid and arachidonic acid metabolism, thereby inhibiting inflammation and oxidative stress. This study is expected to provide research foundation for the development of ABS as a new anti-aging drug.

Keywords: Aging, Anshen bunao syrup, Inflammation, Metabolomics, Oxidative Stress, Transcriptomics

Received: 17 Dec 2025; Accepted: 16 Feb 2026.

Copyright: © 2026 Sun, Xia, Wu, Liu, Wang, Yan, Jin, Zhang, XIAO and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Huizi Jin
Xiuyun Zhang
XUE XIAO

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Supplementary Material