ORIGINAL RESEARCH article
Front. Allergy
Sec. Allergy Diagnosis
Volume 6 - 2025 | doi: 10.3389/falgy.2025.1525012
Tissue-based skin prick test extracts from Atlantic salmon containing occupationally relevant allergens
Provisionally accepted
- 1University Hospital of North Norway, Tromsø, Norway
- 2UiT The Arctic University of Norway, Tromsø, Troms, Norway
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Introduction: Diagnosis of salmon allergy often includes skin prick testing using commercially available extracts. Multiple studies have shown that these may contain highly variable amounts of important allergens. In this study we aimed to produce skin prick test extracts containing salmon allergens relevant to the occupational exposure situation. The extracts were characterized with respect to the presence of known salmon allergens. The effect of heat-treatment on the antibodybinding of allergens was also assessed.Method: Allergenic proteins were extracted from muscle, skin, and outer mucus coating from Atlantic salmon (Salmo salar). Electrophoresis was used for protein separation with subsequent silver staining and immunoblotting for the detection of known allergenic proteins with antibodybinding activity to commercially available antibodies. The identity of allergens was confirmed by mass spectrometry.Results: Our extraction methods were successful in detecting the allergenic proteins aldolase, collagen, enolase, and parvalbumin from muscle, skin, and outer mucus coating from Atlantic salmon. Our work also demonstrates that these allergens have various tolerance towards heating. Conclusion: By using multiple fish tissues for extraction, we were able to produce skin prick test extracts containing allergens of presumed relevance in diagnosis of work-related sensitization.
Keywords: Allergen extracts, occupational allergy, salmon allergy, salmon processing, Skin prick testing, Thermolabile allergen
Received: 08 Nov 2024; Accepted: 09 Jun 2025.
Copyright: © 2025 Elda, Grgic, Stensvåg and Bang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Berit Bang, University Hospital of North Norway, Tromsø, Norway
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