BRIEF RESEARCH REPORT article
Front. Allergy
Sec. Mechanisms in Allergy
This article is part of the Research TopicDust mite allergy: Immunological mechanisms, Environmental impact, Genetic susceptibility, Diagnosis, Therapeutic strategies & Preventive measuresView all articles
Investigation of Neutrophil Infiltration in the Acute Canine Atopic Dermatitis Model
Provisionally accepted
- North Carolina State University, Raleigh, United States
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Atopic dermatitis (AD) is an inflammatory skin condition associated with chronic itch and inflammation in both humans and animals. While this disease depends upon various immune cell types, the precise role and kinetics of neutrophils remain elusive, particularly in relevant large-animal models. Given a recent report suggesting the involvement of neutrophils in a mouse model, we aimed to characterize the temporal presence and infiltration of these cells in a canine model of house dust mite (HDM)-induced AD. AD was induced in canines via HDM exposure, and skin biopsies were analyzed over a time course (0–96 hours) using standard H&E staining and specific immunofluorescent (IF) staining for canine neutrophils. Our results showed general cellular infiltration with the H&E method, while IF further confirmed detectable neutrophil immunoreactivity starting between 24 and 96 hours post-challenge in atopic skin. Quantitation demonstrated a significant increase in neutrophil infiltration (cells/mm²) in atopic skin at 48 hours following HDM exposure compared to baseline (p = 0.041). Collectively, our data confirms time-dependent infiltration of neutrophils into the skin of the canine AD model following allergen challenge, supporting the hypothesis that this previously overlooked immune cell may play a role in the acute phase of AD pathogenesis and sensitization.
Keywords: dog, Canine atopic dermatitis, House dust mite, Neutrophil, Skin
Received: 01 Oct 2025; Accepted: 27 Nov 2025.
Copyright: © 2025 Mochizuki and Mishra. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Santosh K. Mishra
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