BRIEF RESEARCH REPORT article
Front. Anim. Sci.
Sec. Animal Physiology and Management
Volume 6 - 2025 | doi: 10.3389/fanim.2025.1603264
This article is part of the Research TopicEconomic Impact of Infections on the Farm IndustryView all 6 articles
Development of a TaqMan-based quantitative real-time PCR for the detection of Porcine circovirus type 3
Provisionally accepted
- 1Xichang University, Xichang, China
- 2Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd., Dezhou, China
- 3Zhejiang University, Hangzhou, Zhejiang Province, China
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Porcine circovirus type 3 (PCV3) is a recently identified member of the porcine circovirus family, primarily associated with conditions such as dermatitis and nephropathy syndrome, reproductive failure, and multisystem inflammatory lesions in swine. There has been a significant increase in the prevalence of PCV3 in China, attracting considerable attention. Consequently, there is an urgent need for a highly sensitive, cost-effective, and efficient method for the detection of clinical samples. This study developed a TaqMan-based quantitative real-time PCR (TaqMan-qPCR) assay utilizing specific probes and primers designed based on the PCV3-REP gene. Following the optimization of reaction conditions, sensitivity analysis determined that the detection limit of this method was 7.3 × 10 0 copies/µL. Specificity analysis demonstrated no cross-reactivity with other common porcine pathogens, underscoring its specificity. Furthermore, the inter-and intra-assay coefficients of variation were both less than 1%, indicating high reproducibility. A total of 2,454 clinical samples were collected and analyzed using the developed method. The findings revealed that the prevalence of PCV3 was highest in testicular fluid samples, with a rate of 71.28% and the lowest detected Cq values among all sample types, indicating a significant likelihood of vertical transmission of PCV3. Additionally, oral fluid samples exhibited the second highest positive rate at 59.83%, highlighting the importance of monitoring infection rates in fattening pig herds from a veterinary perspective. In conclusion, this study successfully developed a highly sensitive and specific TaqMan-qPCR method, which is effective for detecting PCV3 across a variety of clinical samples.
Keywords: Porcine circovirus type 3, rep gene, TaqMan-qPCR, Clinical sample, Detection rate
Received: 31 Mar 2025; Accepted: 23 Jun 2025.
Copyright: © 2025 Hu, Lai, Xu, Guan, Li, Zhang, Hao and Yan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Guangwen Yan, Xichang University, Xichang, China
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