ORIGINAL RESEARCH article
Front. Bioinform.
Sec. RNA Bioinformatics
Volume 5 - 2025 | doi: 10.3389/fbinf.2025.1625145
Comparative transcriptome analysis of different tissues of Hylomecon japonica provides new insights into the biosynthesis pathway of triterpenoid saponins
Provisionally accepted
- School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
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Triterpenoid saponins are one of the main activities of roots and rhizome of Hylomecon japonica, with various pharmacological activities such as antibacterial, anticancer, and anti-inflammatory. To elucidate the biosynthesis pathway of triterpenoid saponins in H. japonica, DNA Nanoball sequencing technology was used to analyze the transcriptome of leaves, roots, and stems of H. japonica. Out of a total of 99,404 unigenes, 78,989 unigenes were annotated by seven major databases; 49 unigenes encode 11 key enzymes in the biosynthesis pathway of triterpenoid saponins.Nine transcription factors were found to be involved in the metabolism of terpenoids and polyketides in H. japonica and a spatial structure model of squalene synthase in triterpenoid saponin biosynthesis was established. This study greatly enriched the transcriptome data of H. japonica, which is helpful for further analysis of the functions and regulatory mechanisms of key enzymes in the biosynthesis pathway of triterpenoid saponins.
Keywords: Hylomecon japonica, Transcriptome sequencing, Triterpenoid saponins, Squalene Synthase, Differentially expressed genes
Received: 08 May 2025; Accepted: 24 Jun 2025.
Copyright: © 2025 he, xu, xu, fang and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Qingshan Yang, School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
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