Reason for Erratum:
Due to a typesetting error, a misalignment in Table 2 lead to the publication of incorrect information. In the “Resolving gel” column, line “1.5M TRIS-HCl, pH 8.8,” the volume should be 4.5 mL, and not 4.5 L as published.
Table 2
| Stacking gel | Resolving gel | 10X Running Buffer | Light gel | Heavy gel | Moving solution | Conservation buffer | DTT solution | Iodoacetamine solution | Equilibration buffer | 5X running buffer | Overlays solution | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 40% acrylamide/bisac rylamide (37.5:1) (w/v) | 1 mL | 6.3 mL | ||||||||||
| 1.5M TRIS-HCl, pH 8.8 | 4.5 mL | 52.5 mL | 52.5 mL | 50 mL | 100 mL | 50 mL | ||||||
| 0.5M TRIS-HCl, pH 6.8 | 2.5 mL | |||||||||||
| 10% SDS (w/v) | 0.1 mL | 0.18 mL | 2.1 mL | 2.1 mL | 4 mL | |||||||
| 10% APS | 0.05 mL | 0.09 mL | 2.1 mL | 1.05 mL | ||||||||
| TEMED | 0.015 mL | 0.009 mL | 360 μL | 60 μL | ||||||||
| Glycine | 120 g | |||||||||||
| SDS | 40 g | 20 g | 40 g | |||||||||
| TRIS | 576 g | 60.5 g | ||||||||||
| 30% acrylamide/bisac rylamide (37.5:1) (w/v) | 68 mL | 128 mL | ||||||||||
| Glycerol | 18 mL | 74 g | 378 g | |||||||||
| 1% bromophenol blue | 210 μL | 20 μL | ||||||||||
| 1M DTT | 0.1 g | |||||||||||
| Iodoacetamine | 1.12 g | |||||||||||
| Equilibration buffer | 10 mL | 25 mL | ||||||||||
| Urea | 355 g | |||||||||||
| Agarose type I-A | 0.4 g | |||||||||||
| Agarose type VII | 0.1 g | |||||||||||
| 5X running buffer | 20 mL | |||||||||||
| Apyrogenic water | 6.35 mL | 6.93 mL | to 4L | 85 mL | 14 mL | 90 mL | 296 mL | To 1L | to 4L | 80 mL |
Detailed protocol for gel and electrophoresis experiments.
Acrylamide/bisacrylamide (37.5 :1) solution (w/v) (BioBasic Inc, Markham, Ontario, Canada), agarose type I-A (Sigma, Oakville, Québec, Canada), ammonium persulfate (BioBasic Inc, Markham, Ontario, Canada), bromophenol blue (Sigma, Oakville, QC, Canada), dithiothreitol (DTT) (BioBasic Inc, Markham, Ontario, Canada), glycerol (BioBasic Inc, Markham, Ontario, Canada), glycine (BioBasic Inc, Markham, Ontario, Canada), iodoacetamide (BioBasic Inc, Markham, Ontario, Canada), sodium dodecyl sulfate (SDS) (BioBasic Inc, Markham, Ontario, Canada), TRIS (BioBasic Inc, Markham, Ontario, Canada), TRIS-HCl (BioBasic Inc, Markham, Ontario, Canada), tetramethylethylenediamine (TEMED) (BioBasic Inc, Markham, Ontario, Canada), urea (BioBasic Inc, Markham, Ontario, Canada), 2- hydroxythylagarose type VII (Sigma, Oakville, Québec, Canada).
The publisher apologizes for this error and the correct version of Table 2 appears below.
This error does not change the scientific conclusions of the article in any way.
Summary
Keywords
secretomic, 2D-DIGE, secreted proteins, secretome, fibrolast-conditioned media
Citation
Frontiers Production Office (2016) Erratum: An Optimized Approach to Recover Secreted Proteins from Fibroblast Conditioned-Media for Secretomic Analysis. Front. Cell. Neurosci. 10:107. doi: 10.3389/fncel.2016.00107
Received
11 April 2016
Accepted
11 April 2016
Published
25 April 2016
Approved by
Cellular Neuroscience Editorial Office, Switzerland
Volume
10 - 2016
Updates
Copyright
© 2016 Frontiers Production Office.
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