Abstract
Axons convey information in neuronal circuits via reliable conduction of action potentials (APs) from the axon initial segment (AIS) to the presynaptic terminals. Recent experimental findings increasingly evidence that the axonal function is not limited to the simple transmission of APs. Advances in subcellular-resolution recording techniques have shown that axons display activity-dependent modulation in spike shape and conduction velocity, which influence synaptic strength and latency. We briefly review here, how recent methodological developments facilitate the understanding of the axon physiology. We included the three most common methods, i.e., genetically encoded voltage imaging (GEVI), subcellular patch-clamp and high-density microelectrode arrays (HD-MEAs). We then describe the potential of using HD-MEAs in studying axonal physiology in more detail. Due to their robustness, amenability to high-throughput and high spatiotemporal resolution, HD-MEAs can provide a direct functional electrical readout of single cells and cellular ensembles at subcellular resolution. HD-MEAs can, therefore, be employed in investigating axonal pathologies, the effects of large-scale genomic interventions (e.g., with RNAi or CRISPR) or in compound screenings. A combination of extracellular microelectrode arrays (MEAs), intracellular microelectrodes and optical imaging may potentially reveal yet unexplored repertoires of axonal functions.
Introduction
Intricate operations, performed by neuronal networks, emerge from the orchestrated interplay of individual neurons. Neurons use action potentials (APs) as a means to encode and relay information from the soma to the presynaptic terminal via reliable conduction through the axon. The three functional compartments of the axon include the axon initial segment (AIS), the axon proper, and the presynaptic terminal. Somato-dendritic integration of a number of synaptic inputs at the AIS are thought to shape the AP firing patterns. The axon proper is often conceived as a simple cable, whose function is the faithful transmission of the AP to distant presynaptic terminals in a digital (all or none) fashion. However, with the development of modern techniques that can directly access small axonal structures, an increasing body of work has emerged that challenges the traditional view on the role of the axon being purely limited to the transmission of the AP(; Sasaki et al., 2011; Sasaki, 2013; ). It has been shown that the shape of the presynaptic AP can be modulated by subthreshold potentials, which, in turn, modulate the spike-evoked transmission through so-called “analog-digital facilitation” (; ; ; ; ; Sasaki, 2013; ). As AP propagation and synaptic transmission might undergo substantial modulation, the computational repertoires of single axons in the neuronal circuit may be more complex than commonly assumed.
Axonal membrane excitability and conduction velocity can change substantially with repeated activation. This can potentially alter the temporal patterns of spikes during propagation from the AIS to presynaptic sites. Such changes in temporal spike patterns may be an important feature of neural coding strategies (; ; ). Axonal conduction velocity in unmyelinated axons depends on several biophysical factors, such as ion-channel densities and kinetics, membrane capacitance, axial resistance, axon geometry, and, for myelinated axons, myelin thickness and internodal distances (; Manor et al., 1991; Shepherd and Harris, 1998; ; ; ). Axonal conduction velocity per se provides little information about the functional aspects of neuronal communication. On the other hand, axonal conduction delay, which depends on both, conduction velocity and axonal length, may have important functional implications in the integration of sensory information (). A plethora of diverse neurological disorders is associated with impaired axonal functionality (Suter and Scherer, 2003; Waxman, 2006; ; ; ; ). Axonal dysfunction can be caused by missing or reduced myelination (e.g., multiple sclerosis) (Steinman, 1996; Trapp et al., 1999). Acute axonal damage (e.g., traumatic injury) (Smith and Meaney, 2000; ), toxic entities, aggregated proteins, microgliosis and disrupted axonal transport (e.g., prion disease, Parkinson’s disease, Alzheimer’s disease) (Liberski and Budka, 1999; Millecamps and Julien, 2013) may directly affect axonal physiology. Lastly, abnormalities in the composition or function of ion channels (in channelopathies, e.g., in certain forms of migraine and epilepsy) are known to alter the conduction properties of axons (; Oyrer et al., 2018; Pietrobon, 2018).
Recent advances in the understanding of axon physiology and pathophysiology have been driven by technological developments, such as optical imaging of the membrane potentials using genetically encoded voltage indicators (GEVI), subcellular patch-clamp recordings from thin axons and boutons, and high-density microelectrode array recordings (HD-MEA) (Figure 1; Shu et al., 2006; ; Sasaki et al., 2011; ; Novak et al., 2013; ; ; Müller et al., 2015; Rama et al., 2015a; Radivojevic et al., 2017). These techniques stand apart from other classical electrophysiological methods in their ability to monitor and interactively control subcellular components of single neurons at high spatial and microsecond temporal resolution. The above-mentioned techniques and devices were reviewed in-depth elsewhere (; Sasaki, 2013; Spira and Hai, 2013; Obien et al., 2015; ; Ohura and Kamiya, 2016; Yang and St-Pierre, 2016; Xu et al., 2017; Zeck et al., 2017; Platisa and Pieribone, 2018; Rama et al., 2018; Wang et al., 2019). Here, we will briefly introduce these technologies with the focus on studying axonal signals, while we describe - in a bit more detail - recent investigations in axonal neurobiology by using HD-MEAs.
FIGURE 1
Technological Approaches
In the following, we provide a brief overview of the three most commonly used recording modalities for measuring axonal signals. We describe the parameters that govern to the detection of neuronal signals and outline recent advances, including potential advantages and limitations. Table 1 lists the key specifications of each methodology. Since several publications are available on each technology, we pooled the data from the most advanced and most recent publications. Given the plethora of applications for each methodology, we will restrict our comparison to the detection and measurements of AP propagation. A schematic overview on the different methodologies along with representative signals is displayed in Figure 1.
Table 1
| Genetically Encoded Voltage Indicators | Subcellular Patch-clamp | CMOS HD-MEA | |
|---|---|---|---|
| Recording type | Optical | Electrical | Electrical |
| Modality | Fluorescence | Intracellular/ Extracellular | Extracellular |
| Signal | ΔF/F | μV, mV, pA | μV |
| Spatial resolution within detection area | 3.25 μm in 1.2 × 3.3 mm2 | NA | 17.5 μm pixel in 3.85 × 2.10 mm2 |
| Temporal resolution | 0.37–1.2 ms | μs | μs |
| Dynamic range | 12–90% ΔF/F per 100 mV | ± 1 V | ±3 μV to ± 1.6 mV c |
| 1 to 200 nA a, 0.1 to 20 nA b | |||
| Device noise | 2.2 mV at 300 W/cm2 1.5 mV at 800 W/cm2 | ∼40 μVRMS 3.0 pARMSa, 1.4 pARMSb | 2.4 μVRMS (AP band) |
| Duration of stable recording | Minutes | Hours | >Months |
| Simultaneous recording sites | Medium; dozens | Low; two sites | High; Thousands |
| Stimulation capability | No – Needs external stimulation | Yes - Electrical | Yes - Electrical |
| Advantages | Non-invasive | Single-spike resolution | Non-invasive |
| Cell-specific labeling | Precise spike shape | Long-term recording | |
| tracking | Single-trial APs | ||
| Subthreshold and PSP detection | High throughput | ||
| Limitations | Photobleaching | Invasive | Cannot detect subthreshold signals |
| Phototoxicity | Time consuming | ||
| Multiple-trial APs | Low throughput | ||
| Labor intensive | |||
| References | ; | Sasaki et al., 2011; | Müller et al., 2015; Radivojevic et al., 2017 |
Comparison of the three techniques in studies that showed AP propagation.
For subcellular patch-clamp, the device specifications of the Multiclamp 700 b have been taken. Feedback resistors: a 50 MΩ, b 500 MΩ. cWith a gain of 1024; up to 1 V with reduced gain.
Voltage Imaging
In order to monitor neural activity at single-cell resolution, optical methods, such as voltage-sensitive dyes and GEVI, make use of fluorescence signals to detect alterations in voltage (Peterka et al., 2011; Storace et al., 2016). Voltage-sensitive dyes have provided important insights into neuronal electrical signaling ranging from individual neurons to population dynamics (; ; Petersen et al., 2003; Miller et al., 2012; Popovic et al., 2015). Yet, major limitations of voltage-sensitive dyes include cell toxicity, phototoxicity, indiscriminate neuronal and glial staining, and small signal-to-noise ratio (SNR) (; Mennerick et al., 2010).
In the last two decades, considerable efforts have been made to overcome these limitations, which have led to the development of GEVIs. The three main molecular designs of GEVIs – inserted into the plasma membrane – are (1) the fusion of fluorescent proteins (FP) to voltage-sensing domains, (2) the use of opsins, and (3) hybrid opsin-FP pairs (rhodopsin-FRET sensor) (; ; ; ; Tsutsui et al., 2013; ; St-Pierre et al., 2014; ). Voltage sensitivity (dynamic range, %) is an important parameter of fluorescence indicators, expressed as ΔF/F per 100 mV (−70 to 30 mV), which represents linear changes in fluorescence in response to voltage fluctuations. To detect neuronal activity with high SNR, a combination of key features of voltage indicators, such as bright fluorescence, fast kinetics (rapid response to changes in voltage), large dynamic range, photostability, and efficient plasma membrane localization is desired (; Xu et al., 2017; Piatkevich et al., 2018; Wang et al., 2019).
While there is constant development of new constructs, a recent work by provided a temporal snapshot of state-of-the-art GEVIs and compared their performances. The authors report that QuasAr2, a rhodopsin-based GEVI, outperforms other GEVIs concerning the optical detection of single APs of neurons in vitro, featuring high signal amplitude, fast kinetics and high SNR. In addition, Ace-2N-4AA-mNeon, a rhodopsin-FRET sensor, was shown to resolve individual spikes in single trials without averaging, while suffering from fast photobleaching.
A combination of a red-light-excited QuasAr2 with a spectrally compatible blue-light-activated channelrhodopsin (CheRiff) was co-expressed in neurons via a vector called “Optopatch,” which was targeted at enabling simultaneous all-optical electrophysiology in neuronal cultures or organotypic brain slice cultures (; ). The use of this construct enabled mapping of the dynamics of AP initiation and propagation across dendritic and axonal structures at high spatiotemporal resolution (Figure 1). However, significant multi-trial averaging (200–17,000 trials) was required for attaining good enough signal-to-noise characteristics ().
Subcellular Patch-Clamp Recordings
Patch-clamping is the gold standard technique for studying electrical properties of neurons at unprecedented resolution. The patch-clamp technique uses a glass micropipette that presses against the cell membrane to form a tight gigaohm seal resistance between the cell membrane and the rim of the glass micropipette. In the original cell-attached configuration, activity of single ion channels in the tiny patch of membrane surrounded by the tip of the pipette can be studied. If the patch of membrane under the pipette tip is ruptured by applying pressure, the electrode accesses the inside of the cell in the so-called whole-cell configuration, where the trans-membrane voltage and currents can be directly recorded (Neher and Sakmann, 1976; Ogden and Stanfield, 1994).
Most patch-clamp studies have been conducted on the soma, which is the largest compartment of a neuron (8–30 μm in diameter). One of the limitations of the conventional patch-clamp technique is that the studies on axons encountered technical difficulties due to the thin axonal structure (∼200 nm in diameter). Accordingly, axonal recordings have been mostly restricted to giant axon terminals, such as hippocampal mossy fiber boutons (; ; ) and the Calyx of Held (; ; ). Recordings from thin axons have been obtained from axonal blebs (3–6 μm), which are resealed swellings at the cut ends of axons after brain slicing procedures (Shu et al., 2006, 2007; ; ; Rama et al., 2015a). Recently, recordings from intact thin axons have been made possible using a fluorescence-guided patch-clamp technique (; Sasaki et al., 2011, 2012; ; ). Cell-attached extracellular recordings of APs in intact unmyelinated axons (∼1 μm in diameter) have been made using pipettes coated with fluorescently conjugated albumin. However, stable recording was possible for only less than 60 min with ∼50% success rate. Simultaneous whole-cell recordings have been performed from the soma and axon shaft of hippocampal basket cells in acute slices () as well as in the presynaptic terminals in cerebellar Purkinje cells in cultures () to examine the fidelity of AP propagation.
In recent years, several studies were conducted using paired recordings from two distinct sites along a single axon or from a presynaptic axon terminal and a postsynaptic neuron. These experiments have made considerable contributions to understanding the mechanism of analog-digital facilitation, compartmentalized distribution of ion channels and gating properties, as well as the modulation of short- and long-term synaptic plasticity (; ; Sasaki et al., 2011; ; ; Rama et al., 2015b; Rowan et al., 2016). However, due to the limitation in simultaneously recording from multiple sites along the axon, the patch-clamp technique is not capable of tracking the modulation of AP propagation throughout the axon proper.
CMOS HD-MEAs
The electrical activity of neurons can also be detected extracellularly by means of metal electrodes, arranged in large arrays and known as MEAs. Microelectrodes can record changes in the electric field generated by the moving ions in the extracellular space during the electrical activity of a nearby neuron (; ). During an AP, the fast Na+ current flows away from the electrode into the cell and results in a negative peak in the extracellular action potential (EAP). Thereafter, a slower current of K+ ions flows out of the cell toward the electrode resulting in a positive peak. Most axonal signals show a stereotypical positive-first, triphasic shape. The first, small amplitude positive peak corresponds to a capacitive current, the large negative peak to the Na+ current, and the final positive peak to the K+ current (). In general, EAPs show heterogeneity in signal shapes and amplitudes depending on the magnitude, polarity, and the distance from the recording site (Nunez and Srinivasan, 2006). In addition, the relative positioning of cells with respect to the location of electrodes has a strong influence on the amplitude of the EAP (). EAPs signal amplitudes are in the range of μV and are usually around three orders of magnitude lower than intracellularly measured signals (mV) ().
Commercially available standard MEAs are an established technology for investigating neuronal network activity. However, they do not allow for targeting individual neurons in a network due to the limited number of electrodes (<300), arranged at a comparably large pitch (>30 μm) (; ; Stett et al., 2003; Rutten et al., 2007). In order to investigate the properties of individual neurons, CMOS (complementary metal oxide semiconductor)-technology-based, planar, HD-MEAs can be used that enable simultaneous recording from a large number of sites at high spatiotemporal resolution (; ; ; ; ; ; ; Ogi et al., 2017; Tsai et al., 2017). In contrast to a full-frame readout that is also used with CMOS cameras, our lab has developed a flexible readout approach, where a matrix of switches below the electrodes (total number: 26,000–59,000 electrodes) routes arbitrarily selectable subsets of 1024 or 2048 electrodes to a high-end readout circuitry placed outside the electrode array. This flexible readout approach enables (i) high spatial resolution with electrode densities of 3,000 to 5,000 electrodes per mm2 at (ii) good signal quality. However, not all the electrodes can be simultaneously read out, but only subsets in a sequential approach [for technical details, cf. (; Müller et al., 2015; ; Viswam et al., 2018; Yuan et al., 2018)].
The main advantage of the HD-MEAs is the high spatiotemporal resolution, which allows for detection of signals from thin axons (∼200 nm diameter) and the ability to record APs at microsecond temporal resolution. This degree of resolution helps to efficiently assign detected extracellular spikes to units or neurons through spike sorting (; ). HD-MEAs enable, owing to the high spatial resolution and non-invasive detection of EAPs, the simultaneous recording at EAPs at hundreds of sites simultaneously along the axonal arbor for up to several days (; Radivojevic et al., 2017). CMOS-based HD-MEAs have been used with many in vitro preparations, such as dissociated cell cultures (Maccione et al., 2012; ; Yada et al., 2016; ; Radivojevic et al., 2017), cultures of induced pluripotent stem cells (iPSCs) (; ), acute retinae (Menzler and Zeck, 2011; ; ), acute brain slices (; ; ; Medrihan et al., 2014; Obien et al., 2019), and organotypic brain slice cultures (). A disadvantage of HD-MEAs is that inferences with respect to analog signaling are difficult, as the subthreshold signals are not directly measurable.
Studies of Axonal Neurobiology Using HD-MEAs
The possibility to stimulate and record from a single axon, simultaneously at multiple spatial locations, enables to study axonal electrical properties in great detail. Capitalizing on this capability, our group investigated the possibilities to study neuronal cultures by using a combination of HD-MEA recordings, electrical extracellular stimulations, live staining of neurons directly on the HD-MEA, and patch-clamping of targeted individual neurons. For a long time, the soma and dendrites have been considered the main contributors to the EAP landscape, since most electrophysiological measurements, e.g., through whole-cell patch-clamp, have been done at the soma. Although the initiation of APs has been known to occur at the distal AIS (; ; ; Popovic et al., 2011; ; ; ), the contribution of axons to the EAP landscape has been assumed to be small, if not negligible due to the small dimensions of axonal structures – in contrast to the soma and dendrites. In order to investigate the contribution of different neuronal compartments to the EAP spatial landscape in detail, our group has used HD-MEAs to electrically image EAPs of cultured cortical neurons and of Purkinje cells in acute cerebellar slices (). By using spike-triggered averaging, the EAP landscapes of more than 50 neurons were measured and compared to fluorescence images of the respective neuronal cells (). We found that the largest measured EAP signal amplitudes originated from the AIS, instead of the soma. The dominant EAP signals, found at the AIS, featured negative polarity (charges entering the cell), while some EAP signals found in nearby dendrites had positive polarity (return currents or charges exiting the cell) (Figure 2A,B). These findings are relevant in interpreting results obtained with extracellular recording schemes (in vitro and in vivo), for setting up compartmental neuron models, and for developing methods to study the function of the AIS in healthy and diseased cellular cultures.
FIGURE 2
A characteristic parameter of axons is the conduction velocity, which determines how fast information is transferred between neurons. Detecting fluctuations or deviations in conduction velocity along the axon can provide an understanding of factors that affect conduction success or failure. Such detection poses a major challenge, as it requires a method to directly measure AP propagation at several points along the axon. Several groups have utilized PDMS tunnels, combined with MEAs, to confine the axons, to increase SNR (higher electrical resistance along the channels) and to track the AP propagation along axons and axonal bundles (Shimba et al., 2014; Lewandowska et al., 2015; ). Our group used stimulus-triggered averaging of EAPs to precisely measure the propagation of APs and quantify the conduction velocity along axonal branches (). In general, the velocity of AP propagation along axons of wild-type primary rodent neuron cultures increases with age, as observed in our experiments and reported by other authors (; ). Both, antidromic (toward the soma) and orthodromic (away from the soma) AP propagations featured variations in conduction velocity along the axon (Figure 2C). The variations persisted upon application of synaptic blockers (100 μM APV, 10 μM CNQX, and 50 μM bicuculline methiodide), suggesting that variations in ion-channel properties and densities influence the conduction properties of axons, among others factors. Moreover, higher conduction velocities were observed in axonal segments closer to the soma as compared to the putatively thinner distal branches, which is in agreement with the theory that the action-potential propagation velocity is inversely proportional to the axon diameter (). Pathological conditions affecting the axon may cause conduction delays, so that the capability to measure axonal signal propagation may allow for phenotyping cell cultures or brain slices that are characteristic of brain disorders and for identifying pharmacological effects.
The electrical properties of neurons, including their susceptibility to extracellular electrical stimulation, are highly variable across their morphology, so that stimulation efficiency with extracellular electrodes will strongly depend on where the neuron is stimulated. By combining optical imaging and electrically multisite stimulation, we could determine the electrical stimulation profiles of single neurons (Radivojevic et al., 2016). The AIS, the axonal arbor, and proximal somatodendritic compartments could be identified as prime stimulation targets (Figure 2D). Stimulation at the AIS required low voltages and provided immediate, selective and reliable neuronal activation, whereas stimulation at the soma required high voltages and produced delayed and unreliable responses. Subthreshold stimulation at the soma depolarized the somatic membrane potential without eliciting APs. These findings provided a strategy to stimulate individual neurons with high specificity, by first measuring their EAP footprint to determine the likely location of their AIS (region of highest signal amplitudes) for subsequent electrical stimulation with low voltages.
A property of axons, which is of high biological relevance but is very hard to experimentally investigate, is the temporal precision with which axons conduct APs. The dendritic integration depends on the timing of incoming postsynaptic potentials. However, determining the temporal precision of axonal conduction, again, requires experimental access to a single axon at multiple locations and requires resolving single APs. As discussed previously, most recording modalities lack either the spatial or temporal resolution, or they rely on averaging many APs. Averaging, however, is not an option, when the timing of individual APs needs to be estimated. We demonstrated a method to non-invasively and directly record individual APs propagating along axons at microsecond temporal resolution using HD-MEA recordings and a template-matching technique relying on multi-electrode templates. We were able to detect individual APs propagating across entire neurons including axonal terminals, which were hundreds of micrometers away from the AIS by using optimized matched filters (Radivojevic et al., 2017). We found that cortical axons conduct single APs with high temporal precision and reliability. Individual APs travel along 1 mm of axons with a fixed travel time ± 100 μs, and we did not observe any conduction or branch-point failure in more than 8,000,000 recorded APs (Figure 2E,F).
Discussion and Outlook
Based on the studies reviewed above, we think that HD-MEAs constitute a versatile tool to investigate neuronal information processing and axonal signaling. The possibility to conduct long-term, simultaneous, multisite recordings at high spatiotemporal resolution renders HD-MEAs an ideal technology for detailed characterizations of neurons and axons. HD-MEAs can be used to study alterations in axonal signal propagation and the effects of brain disorders on axonal signaling, as they provide a direct functional readout. Examples include the assessment of the effects of mutations in voltage-gated ion channels (e.g., in the case of channelopathies) on signal propagation velocity. The possibility to reliably electrically stimulate neurons at high frequencies can be used to study the modulation of axonal APs and the mechanisms of axonal conduction failures during repetitive neuronal activation (; ; ). Furthermore, spatiotemporal aspects of analog-digital integration in axonal signals can be investigated (; Shu et al., 2006).
HD-MEAs can be arranged in a multi-well-plate format to realize high-throughput as required, e.g., for large-scale genomic interventions (RNAi or CRISPR) or compound screenings, using human iPSC-derived neurons to model neurological diseases. HD-MEAs enable access to a variety of electrophysiological parameters, including axonal properties, which can be used for characterizing functional phenotypes of neurological disease models. Therefore, HD-MEAs can be used as a platform for drug screening, pre-clinical diagnostics and will find applications in the evolving landscape of precision medicine.
From a technological perspective, it is conceivable that only the combination of different recording modalities will substantially increase the number of applications. Extracellular, intracellular and optical readouts can be combined to determine how they can complement each other. For example, subthreshold voltage distributions can be optically visualized while simultaneously measuring axonal signals throughout the axonal arbors using HD-MEAs. Such an approach will allow for deciphering effects of axonal and synaptic plasticity in neuronal networks and provide functional insights into axon physiology and, possibly, pathophysiology. In particular, it may become possible in the future to better understand the functional underpinnings of clinically heterogeneous diseases that arise from axonal disturbances.
Statements
Author contributions
VE, FF, MO, and AH wrote the manuscript. VE built the figures.
Funding
This work was supported by the European Community through the European Research Council Advanced Grants 694829 “neuroXscales” (Horizon 2020) and the Swiss National Science Foundation Grant 205321_157092/1 (“Axons”). FF received individual support by the Swiss National Science Foundation “Ambizione” Grant PZ00P3_167989. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Acknowledgments
M. Emmenegger and E. J. Rushing, both at the University of Zurich, are acknowledged for their help with the manuscript and proofreading.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Summary
Keywords
axon, action potential propagation, patch-clamp technique, genetically encoded voltage indicators, high-density microelectrode arrays
Citation
Emmenegger V, Obien MEJ, Franke F and Hierlemann A (2019) Technologies to Study Action Potential Propagation With a Focus on HD-MEAs. Front. Cell. Neurosci. 13:159. doi: 10.3389/fncel.2019.00159
Received
28 February 2019
Accepted
08 April 2019
Published
26 April 2019
Volume
13 - 2019
Edited by
Dominique Debanne, INSERM U1072 Neurobiologie des Canaux Ioniques et de la Synapse, France
Reviewed by
Zbili Mickael, Institut National de la Santé et de la Recherche Médicale (INSERM), France; Michael Blake Hoppa, Dartmouth College, United States
Updates
Copyright
© 2019 Emmenegger, Obien, Franke and Hierlemann.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Vishalini Emmenegger, vishalini.emmenegger@bsse.ethz.ch
This article was submitted to Cellular Neurophysiology, a section of the journal Frontiers in Cellular Neuroscience
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