Abstract
Background:
Mutations in lipoxygenase homology domain 1 (LOXHD1) cause autosomal recessive inheritance, leading to high-frequency and intermediate-frequency hearing losses in patients. To date, studies on the localization of LOXHD1 gene expression are limited. In this study, we aimed to observe the expressions of Loxhd1b in zebrafish, C57BL/6 murine cochlea, and HEI-OC1 cells.
Methods:
The expression of Loxhd1b in the auditory system of zebrafish was explored by in situ hybridization experiments of zebrafish embryos. The expression of Loxhd1b in cochlear and HEI-OC1 cells of C57BL/6 mice was analyzed by immunofluorescence staining. Confocal microscopic in vivo imaging was used to detect the number and morphological characteristics of lateral line neuromasts and inner ear hair cells in zebrafish that knocked down Loxhd1b gene. The effect of knockdown Loxhd1b gene on the development of zebrafish otolith and semicircular canal was observed using microscopic. Transcriptome sequencing was used to identify downstream molecules and associated signaling pathways and validated by western blotting, immunostaining, and rescue experiments.
Results:
Results of the in situ hybridization with zebrafish embryos at different time points showed that Loxhd1b was expressed in zebrafish at the inner ear and olfactory pores, while the immunostaining showed that Loxhd1 was expressed in both C57BL/6 mouse cochlea and HEI-OC1 cells. Loxhd1b knockdown causes a decrease in the number of spinal and lateral line neuromasts in the inner ear of zebrafish, accompanied by weakened hearing function, and also leads to developmental defects of otoliths and ear follicles. The results of transcriptomics analysis revealed the downstream molecule brain-derived neurotrophic factor (BDNF) and verified that Loxhd1b and BDNF regulate the formation of zebrafish hair cells by synergistic regulation of BDNF/TrkB/ERK pathway based on western blotting, immunostaining, and rescue experiments.
Conclusion:
This was the first time that the BDNF/TrkB/ERK pathway was identified to play a critical role in the molecular regulation of the development of zebrafish hair cells and the auditory development by Loxhd1b.
Introduction
It has been documented that nearly 500 million people worldwide suffer from sensory impairment due to hearing loss (; ). Although many hearing related genes have been identified, it is important to understand the molecular mechanism regulating the hearing loss. Previous studies have shown that 60% of deaf patients are related to genetic factors (). Based on the patient’s symptoms, genetic hearing losses are divided into two categories, i.e., syndromic (30%) and non-syndromic (70%) (). In addition, there are four types of hereditary hearing loss based on the inheritance, including autosomal recessive inheritance, autosomal dominant inheritance, mitochondrial inheritance, and X-linked inheritance. The most common type of hereditary hearing loss is the autosomal recessive non-syndromic hearing loss (ARNSHL), which accounts for ∼80% of non-syndromic hearing loss (NSHL). However, there are many genes related to deafness, and the genetic methods used to investigate these genes are complex and diverse (; ). The explicit molecular functions of these genes in the formation of deafness largely remain unknown. Studying the pathogenesis of hereditary deafness is extremely challenging and new drugs to protect hearing are also crucial.
The LOXHD1 gene is located in the 18q21.1 region of human chromosome 18, containing 48 exons and encoding the lipoxygenase homology domain containing protein 1 (LOXHD1) with a total of 2,068 amino acids (). The LOXHD1 contains a total of 15 PLAT repeats. The LOXHD1 gene is specifically expressed in the inner ear of mice (). It is first expressed in the nucleus of inner ear hair cells after birth, and then concentrated in the cytoplasm as the development progresses. At the mature stage of the mice, it is specifically expressed on the entire static surface of the cilia of the cochlear hair cells. The inner ear structure of mice with congenital deafness induced by ethylnitrosourea (ENU) appears abnormal, which is manifested as loss of hair cells and degeneration of spiral ganglion cells (). LOXHD1 plays an important role in the mechanical transduction of mature hair cells (). To date, studies on the expression and the effect of Loxhd1b on zebrafish’s auditory development are only limited to the use of mice but not verified by the use of zebrafish. Zebrafish have the advantage of studying hair cell development in vivo. Available data have shown that zebrafish shares approximately 70% of human genes, and more than 80% of known human disease-related genes have their counterparts in zebrafish (). In comparison to the mouse model used in the previous studies of Loxhd1b, zebrafish is used as a biological model due to its visualization advantages, i.e., it is convenient to record and track the developmental process through imaging (; ). Zebrafish are relatively genetically conserved with mice and human, by using which we can observe the auditory system in vivo. In particular, there are hair cells in the inner ear and lateral nerve mound, while the inner ear is located near the surface of the embryo, and there is a lateral nerve mound on the body surface, which is convenient for direct observation and intervention. In this regard, zebrafish has currently become a model organism for studying the development of ear vesicles and nerves that are closely related to human diseases (; ).
As a small protein isolated from pigs for the first time in 1982 (), the brain-derived neurotrophic factor (BDNF) is the most widely distributed neurotrophic factor in the central nervous system (; ), mainly responsible for regulating the growth, development, differentiation, and repair of neurons, with an important regulatory effect on the structure and function of neurons. BDNF has also shown a protective effect on the hair cells and spiral ganglion cells of the inner ear of rodents (). In the brain neurons, BDNF binds to two different receptors, including the high-affinity tyrosine kinase receptor B (Tropomyosin-related kinase B, TrkB) and the low-affinity p75 receptor (; ), to regulate the function of neurons. Studies have shown that in the hair cells of the inner ear and lateral nerve mounds of zebrafish, BDNF is involved in the survival and development of hair cells by binding to TrkB (), while the BDNF can regulate the migration of zebrafish lateral line primordium and the maintenance of mechanoreceptor progenitor cells through the mediation of TrkB (). In the brain neurons, the extracellular regulated protein kinase (ERK) is one of the important molecules of the MAPK family. The combination of BDNF and TrkB can activate the intracellular MAPK/ERK signaling pathway (; ), which plays an important role in regulating axon growth and neuron survival and differentiation. To date, no relationship between Loxhd1b and BDNF has been identified, and the function of the BDNF/TrkB/ERK pathway in the molecular regulation of zebrafish hair cell development and auditory development by Loxhd1b are still unclear.
Although the zebrafish model has been used extensively in the study of pathogenesis of a variety of medical disorders (; ; ; ; ; ; ; ; ; ; ; ), no study of Loxhd1b on zebrafish has been reported, and there is no mechanism of how Loxhd1b regulates hair cell development. In this study, the zebrafish model was used for the first time to reveal the molecular mechanism regulating the auditory development by Loxhd1b. Our results suggested that BDNF bound its specific receptor TrkB to activate the downstream molecule ERK, and the BDNF/TrkB/ERK pathway participated in the molecular regulation of auditory development by Loxhd1b.
Materials and methods
Animals
The zebrafish used in this study was kindly provided by Dr. Liu Dong from Nantong University (Nantong, China). Zebrafish wild-type AB line and Tg (brn3c: GFP) line was fed twice a day and kept in a fully automated zebrafish rearing system (ESEN, China; temperature 28 ± 0.5°C, pH 7.0, and conductivity 500 μS) with a photocycle of 14-h light and 10-h dark. Embryos were placed in 0.003% (w/v) 1-phenyl-2-thiourea (PTU) within 24 h to prevent pigmentation.
The suckling mice used in this study were postnatal 3 days old (P3) C57BL/6 mice. The P3 mice were purchased from the Animal Center of Shandong University (Jinan, China). After inhalation anesthesia, mice were acutely decapitated with dissecting scissors, and then quickly used for dissection and further experiments. Laboratory animal Use Permit [SYXK(LU) 2019 0005].
Cell culture
The HEI-OC1 cells, a commonly used auditory cell line with the characteristics of hair cells, were used to investigate the molecular mechanism of auditory development (; ). The HEI-OC1 cells were cultured in the mixture of 5% volume of FBS without antibiotics and high-sugar DMEM (Gibco BRL, Grand Island, NY, USA) and kept in a 5% CO2 incubator at 33°C.
Whole mount immunohistochemistry
The zebrafish immunohistochemistry experiments were performed based on the methods previously reported (). The antibodies used in this study included Alexa Fluor 488 antibody (1:1,000; Invitrogen), anti-myosin VIIa antibody (1:200; DSHB), Alexa’s anti-SOX2 antibody (1:500; abcam), and Fluor 594 secondary antibody (1:1,000; Invitrogen). Larvae were incubated with DAPI (1:500 dilution; Thermo Fisher Scientific) for 20 min to label the nucleus. Cells were fixed with 4% PFA for 30 min, then permeabilized with 1% Triton X-100 for 5 min and blocked with 1% BSA in phosphate buffered saline (PBS) for 1 h. Then, the cells were incubated with primary anti-Loxhd1 antibody (bs-18343R, Bioss), anti-BDNF antibody (orb318730, biorbyt), and anti-Myosin VIIa antibody (138-1, DSHB) at 4°C overnight, and then with both fluorescent secondary antibody and DAPI (D9542, Sigma) in dark for 1 h, and washed with PBS three times. The samples were observed under a laser scanning confocal microscope (Germany, LEICA).
Whole mount in situ hybridization
The whole-body in situ hybridization (WISH) was performed based on the standard procedures (; ). First, the Loxhd1b specific primers (forward primer 5′-GTGGTGGATGATGAGGAGATG-3′ and reverse primer 5′-TGTCCGACTCGATCACTCTG-3′) and the BDNF specific primers (forward primer 5′-AGGAGTTGCTTGAGGTGGAA-3′ and reverse primer 5′-TATCCATGGTAAGGGCTCGC-3′) were used to amplify the target fragments from zebrafish cDNA and transform into the pGEM-T Easy vector. Then, the DIG RNA labeling kit was used to transcribe the gene-specific digoxin-labeled RNA probe in vitro. The pre-immobilized embryos were incubated with the probe overnight at 4°C. Second, an alkaline phosphatase (AP)-conjugated anti-digoxigenin (Roche, #11093274910) antibody was used to detect the digoxin-labeled RNA probe. The samples were observed with pictures taken with the stereo microscope MVX10 (Japan, Olympus).
Morpholino and mRNA injection
Studies have shown the successful method used to knock down targeted genes in zebrafish using morpholino (MO) (; ; ). Meanwhile, the MO-mediated gene downregulation method has been well applied in Xenopus and zebrafish embryos (; ). However, many limitations in the application of MO are revealed, i.e., the downregulation of gene expression is not achieved through stable genetic manipulation, causing unstable efficiency with the relatively short maintenance time, only about 3 days (; ). Gene Tools was used to synthesize Loxhd1b MO (5′-CAGTGATGCTGAAAGTCTGACTTGC-3′). Embryos obtained from natural mating of Tg (Brn3c: GFP) zebrafish were used for microinjection. The MO was diluted with RNase-free water and injected into embryos at single-cell stage, which were then cultured in egg water medium at 28.5°C.
FM1-43FX staining
Zebrafish hair cells were labeled with viability dye FM1-43FX (Molecular Probes, Invitrogen), which was a fixable analog of N-(3-triethylammonium propyl)-4-(4-(dibutyl) (Amino)-styryl) pyridinium dibromide (FM1-43). FM1-43FX was thought to mark hair cells by traversing mechanically sensitive channels (; ). In this experiment, in order to label the hair cells in neuroma, the larvae were placed in embryo medium containing 0.3 μg/ml FM1-43FX for 30 s, and washed 3 times in fresh embryo medium. Finally, the fish was anesthetized with 0.02% Tricaine (MS-222; Sigma) to observe the hair cells.
Western blot analysis
The HEI-OC1 cells were cultured in a 100 mm cell culture dish, and after the treatment of RNA interference, the cells were collected by centrifugation. Then, PMSF was added to the cytoplasmic protein lysate, and the cells were transferred into 0.22 μm PVDF and incubated with primary antibody BDNF (ab108319; abcam), anti-TrkB (bs-3732R; bioss), anti-ERK (12754S; CST), anti-β-actin (ab8226; abcam) diluted 1:1000 (4°C, overnight), and anti-rabbit IgG secondary antibody (ZB-2301; zsbio) at 25°C for 1.5 h. After washing, the immune complexes were detected using a chemiluminescence imaging system (model: Al480; Bio-Rad).
mRNA sequencing
The total RNA of the samples between Loxhd1b knock-down and wild type zebrafish were extracted with TRIzol reagent (Invitrogen) and analyzed by Agilent 2100 Bioanalyzer (Agilent Technologies, USA). The Illumina HiSeq platform was used to prepare and sequence libraries according to the manufacturer’s instructions (Illumina, USA). The sequences were processed and analyzed by GENEWIZ with the p-value set to < 0.05 to reveal differentially expressed genes (DEGs).
RNA isolation and real-time PCR
Total RNA was extracted from zebrafish embryos by TRIzol reagent according to the manufacturer’s instructions (Thermo Fisher Scientific, USA) with the genomic contaminations eliminated by DNaseI. Total RNA (2 μg) was reversely transcribed using a reversed first strand cDNA synthesis kit (Fermentas, USA) and stored at –20°C. The sequences of PCR primers used for validating the splice-blocking MO were forward primer 5′-GAAAGCGGAGAAGGAGGACT-3′ and reverse primer 5′-CAGCCGTGAACACACTCACT-3′. The housekeeping gene ef1a amplified with forward primer 5′-CTTCAACGCTCAGGTCATCA-3′ and reverse primer 5′-CGGTCGATCTTCTCCTTGAG-3′ was used as internal control to produce a 126-bp amplification product.
C-startle response test
The hearing and balance functions of zebrafish are mainly related to the development of the inner ear (; ). We performed a fast escape reflex and used two near-field pure tone stimuli with different sound intensity levels to perform hearing tests on MO-injected zebrafish (). This experiment was tested in a 96-well plastic plate and recorded with a high-speed camera (Redlake, MotionScope M3, 1000 fps) under infrared light illumination. Larvae in each group was tested 15–18 times with the movement distance after the startled reaction calculated.
Statistical analysis
All data were analyzed using GraphPad Prism 8.0.2. Statistical analysis was performed by one-way analysis of variance (ANOVA) and Student’s t-test or Chi-square test with the p-value < 0.05 considered statistically significant. Each experiment was repeated with 3 or more biological replicates.
Results
Loxhd1b was expressed in zebrafish ear vesicles and olfactory pores
Initially, the results of WISH experiments showed that the expression of Loxhd1b in zebrafish was detected by digoxigenin-labeled Loxhd1b probe (Figure 1). The Loxhd1b gene was highly conserved and was expressed in zebrafish ear vesicles at 24 h post-fertilization (hpf) and over time, was also detected in the olfactory pores (Figure 1A). In addition, the results of real-time PCR (RT-PCR) (Figure 1B) showed that the expression of Loxhd1b was gradually decreased from 24 to 96 h over time, which were consistent with the results by the in situ hybridization.
FIGURE 1
Deficiency of Loxhd1b caused decreased hair cells and neuromasts
Results of gene knockdown analysis showed that MO effectively affected the splicing of Loxhd1b precursor mRNA, resulting in deletion of exon 2 and reading frame shifted (Figures 2A,B). To further investigate the status of hair cells, we observed and counted the number of three inner ear hair cell clusters, i.e., the anterior crest (AC), posterior crista (PC), and lateral crest (LC) (Figure 2C). By using genetically modified fish, we visually evaluated the changes in zebrafish inner ear hair cells after the Loxhd1b gene knockdown. The confocal results showed that 3 days after the injection of Loxhd1b MO, the number of hair cells in each nerve cluster in the lateral line of zebrafish was significantly reduced (Figures 2D,G). In the 72-hpf zebrafish inner ear hair cells, we counted the total number of hair cell clusters of morphant (15) and WT (32) (Figures 2E,I). We also counted the total number of hair cell clusters in the inner ear of zebrafish at 96-hpf of MO (15) and Ctrl (32) (Figures 2E,J). Since the hair cells on the lateral line play a vital role in sensing changes in the surrounding environment, therefore, we further performed WISH experiments based on eya1 specific probes (Figures 2F,H). The results showed that the Loxhd1b gene knockdown caused decreased number of neuromas in zebrafish. Furthermore, we designed a C-shaped startle response to explore the effect of Loxhd1b on zebrafish hearing. Results showed that the movement distance of Loxhd1b-morphant zebrafish C-startle response was significantly lower than that of WT zebrafish (Figure 2K).
FIGURE 2
Because the sensory cells of the lateral neuromound are composed of hair cells and supporting cells, we then explored the effect of the knockdown of Loxhd1b in zebrafish on the formation and maturation of hair cells and supporting cells in lateral neuroma. The results showed that the number of hair cells in the lateral line neuroma labeled with FM1-43FX were significantly lower in Loxhd1b-morphant zebrafish than that in the WT group (Figures 3A,D,E). Meanwhile, we performed the immunohistochemical experiments to label Sertoli cells and hair cells using SOX2 antibody and myosin-7a antibody. The results showed that the number of hair cells in Loxhd1b-morphant zebrafish lateral line neuroma was significantly reduced (Figures 3B,F) as well as the number of support cells (Figures 3C,G).
FIGURE 3
Deficiency of Loxhd1b caused dysplasia of hair cells not due to apoptosis of hair cells but decreased proliferation of support cells
To identify the factors that cause the reduction of zebrafish inner ear and lateral line hair cells, previous studies have reported that the loss of hair cells may be caused by the apoptosis of hair cells (; ; ; ) with the TUNEL test (; ; ; ; ) for diagnosis of apoptosis; alternatively, the loss of hair cells may be due to the abnormal proliferation of support cells, while the hair cells were differentiated from support cells. Therefore, we used TUNEL to test the Loxhd1b knockdown zebrafish model, and found no TUNEL-positive cells, indicating no apoptosis of hair cells (Figure 4). Further studies showed that the abnormal function of Loxhd1b caused the hair cell dysplasia was not due to apoptosis of hair cells but by reduced proliferation of support cell.
FIGURE 4
Deficiency of Loxhd1b caused developmental defects of otic vesicle and otoliths
It was reported previously that the abnormal development of otoliths led to balance disorders (). As pre-in situ hybridization showed significant expression of Loxhd1b in the ear vesicles, we further used confocal microscopy to observe the morphological development of ear vesicles and otoliths in zebrafish at 72 hpf. The results showed that the size of the 72 and 96 hpf ear capsules of Loxhd1b morphant was significantly smaller than the control group (Figures 5A,C). In addition, compared with the control group, the number and shape of otoliths in 77% of Loxhd1b morphant showed a significant reduction or defect (Figures 5B,D).
FIGURE 5
Loxhd1b regulated TrkB/ERK signaling pathway to cause auditory abnormalities
Transcriptome sequencing was performed based on the zebrafish embryos injected with Loxhd1b-morphant at 120 hpf and the control group. The results showed that compared with the control group, a total of 212 and 297 genes were significantly up- and down-regulated in Loxhd1b-morphant, respectively (Figure 6A). These results were further verified by real-time PCR analysis to reveal the difference between the BDNF gene at 72 and 96 hpf Loxhd1b-morphant and the control group, respectively (Figure 6B). The results of the enrichment analysis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database () of these DEGs revealed a total of 30 metabolic pathways enriched, e.g., the MAPK signaling pathway (Figure 6C). In order to explore the relationship between the BDNF/TrkB/ERK signaling pathway and the effect of Loxhd1b on auditory abnormalities, we used the TrkB inhibitor K252a and the ERK inhibitor UO126 to act on the 293T cells. The results of western blot showed that BDNF regulated the activation of its specific receptor TrkB, i.e., the overexpression of BDNF gene enhanced the activation of TrkB, which was required to mediate BDNF’s involvement in Loxhd1b regulation of auditory development, whereas BDNF could not rescue Loxhd1b expression after TrkB was blocked. The results of western blotting experiments further showed that BDNF could regulate the activation of ERK, i.e., the overexpression of BDNF enhanced the activation of ERK, which was required for the regulation of auditory development by Loxhd1b. After the ERK was blocked, BDNF could not rescue the expression of Loxhd1b (Figures 6D–H). These results suggested that the regulation of Loxhd1b expression by BDNF blocked the TrkB/ERK signaling pathway, causing the auditory abnormalities, while Loxhd1b did not affect BDNF expression.
FIGURE 6
BDNF and Loxhd1b were expressed in HEI-OC1 cell line and cochlea of C57BL/6 mice
In order to further determine the relationship between BDNF and Loxhd1b, we first explored the expression location of BDNF in zebrafish tissue by whole embryo in situ hybridization. The results were consistent with those of Loxhd1b, i.e., both BDNF and Loxhd1b were expressed in the inner ear and olfactory pore of zebrafish (Figure 7A).
FIGURE 7
We also detect the localization of both Loxhd1b and BDNF by immunofluorescence analysis with the results observed under confocal microscopy, showing that Loxhd1b and BDNF proteins were highly enriched in hair cell cytosol and slightly distributed in nucleus (Figure 7B). Next, we used 3-day old C57BL/6 mouse basement membrane for immunostaining and specific immunostaining with myosin VII and Phalloidin. The observation under a confocal microscope showed that Loxhd1b and BDNF were specifically expressed in inner and outer hair cells (Figures 7C,D).
BDNF mRNA injection rescued the phenotype induced by zebrafish Loxhd1b gene defect
In order to verify the rescuing effect of the exogenous BDNF injection on the absent phenotype of hair cell development in Loxhd1b morphant, we injected both the synthetic BDNF mRNA and Loxhd1b MO into zebrafish embryos at the single-cell stage in vitro, and performed confocal observations in 3 days. Results showed that the number of hair cell clusters in the ear follicle and lateral line of Loxhd1b morphant were partially restored (Figures 8A–G). Similarly, compared with Loxhd1b morphant, co-injection of BDNF mRNA also partially rescued abnormal phenotypes, including smaller ear follicle size and a decrease in the number of hair cells in the ear follicle (Figures 9A–D).
FIGURE 8
FIGURE 9
Discussion
A growing body of evidence suggests that zebrafish hair cells (HCs) have similar morphology and functions to those in mammals in transmitting sensory information. Therefore, zebrafish lateral line neuroma has become a model for studying factors affecting the development and function of HCs. To date, however, no studies on the effect of Loxhd1b on HCs in zebrafish animal models have been reported in literature. Our study, for the first time, successfully constructed a Loxhd1b-deficient zebrafish model for studying the coordinated control of hair cells at the molecular level.
It has been reported that in the inner ear of Loxhd1Sba/Sba mice, Loxhd1 mRNA is selectively expressed in hair cells, while the protein encoded by Loxhd1 is located in the hair strands (). However, the Loxhd1 expression in zebrafish is unknown. In our study, the results of both in situ hybridization and RT-PCR showed that Loxhd1b was expressed in zebrafish ear vesicles and olfactory pores. The specificity of the expression site suggested that the Loxhd1b gene may affect the development and function of the sensory and balance systems in zebrafish. These results were consistent with those derived from the HEI-OC1 cell line and C57BL/6 mice, indicating that Loxhd1b played an important role in the development of hair cell nerve mounds.
In order to simulate the loss of function of Loxhd1b, we performed the Loxhd1b knockdown experiment in zebrafish using MO. Our results showed that compared with WT, the number of neuromas along the body was reduced and the neuroma lacked hair cells. Loxhd1b was also expressed in the hair cells of the inner ear, showing a temporal expression pattern, indicating that the inner ear and lateral line nerve mounds of the zebrafish were reduced within 3 days. The results of startle response showed that the Loxhd1b-morphant zebrafish swimming behavior was abnormal, indicating the effect of Loxhd1b on the auditory development. These results were consistent with those reported previously, showing that Loxhd1Sba/Sba mice containing PLAT10 repeat missense mutations exhibited hearing loss at 3 weeks of age (). Furthermore, it was reported for the first that our results revealed significant morphological changes in the otolith size and semicircular canal between Loxhd1b-morphant zebrafish in comparison with the control, suggesting that Loxhd1b also played an important role in the balance function of zebrafish.
In general, medium and high frequency sensorineural hearing loss without vestibular disease will lead to stable or progressive hearing loss caused by Loxhd1b (; ; ). The severity of hearing loss varies from mild to profound. However, studies on the Loxhd1 mutations associated with ARNSHL are limited with no functional experiments performed to confirm the pathogenesis of these mutations. Studies have shown that the BDNF gene was down-regulated and closely related to the development of spiral ganglion neurons (; ; ; ; ; ; ; ; ; ). In this work, our results of transcriptome sequencing after the injection of MO showed that there were 509 DEGS (212 up-regulated and 297 down-regulated) in zebrafish after the Loxhd1b knockdown. Notably, the expression of BDNF mRNA was down-regulated by up to 4.9 times in comparison with WT. These results suggested that BDNF was probably a downstream molecule of Loxhd1b, ultimately causing the abnormal auditory development of Loxhd1b. Furthermore, the results of in situ hybridization and immunostaining showed that BDNF and Loxhd1b were co-expressed in hair cells, which was consistent with the results reported previously, showing that the BDNF system existed in the inner ear of zebrafish (). Moreover, the injection of the BDNF mRNA into the 1-cell stage of zebrafish rescued the loss of hair cells and the development of otolith semicircular canals, suggesting that the BDNF may be a downstream molecule of Loxhd1b. Overall, these results demonstrated the novel role of Loxhd1b in both auditory development and balance maintenance in zebrafish.
Lastly, we further explored the molecular mechanism regulating the auditory development by Loxhd1b. Previous studies have shown that BDNF regulates synaptic function by either activating a series of downstream signal pathways, including mitogen extracellular signal-regulated kinase/extracellular signal-regulated protein kinase (MEK/ERK) signal pathway, or through TrkB (; ; ; ; ; ; ; ; ). In the current investigation, it’s the first time we find that the abnormal expression of Loxhd1b regulates BDNF, which can block the TrkB/ERK signaling pathway and cause auditory disorders; at the same time, BDNF positively regulates Loxhd1b. Therefore, we infer that the Loxhd1b gene is closely related to zebrafish hearing, which in turn, provided the potential prevention and treatment of patients with hearing loss in the future.
In summary, our study showed that Loxhd1b played important roles in the zebrafish auditory development, which is closely related to the effects of BDNF receptor and its downstream molecules. It was concluded that BDNF was of great significance for further exploring the treatment of hereditary deafness, providing strong experimental foundation for future development of novel drug and gene therapy.
Statements
Data availability statement
The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: NCBI Sequence Read Archive (SRA): SRR22207161, SRR22207162, SRR22207163, SRR22207164, SRR22207165, and SRR22207166.
Ethics statement
The animal study was reviewed and approved by the Administration Committee of Experimental Animals, Jiangsu Province, China.
Author contributions
DW and XB supervised and designed the project. JL, QZ, and XZ wrote the manuscript. JL, QZ, XZ, and RW analyzed the data. JL, QZ, XZ, RW, and JM performed the experiments. All authors contributed to the article and approved the submitted version.
Funding
This study was funded by the National Natural Science Foundation of China, grant/award number: 81670942, 81470704, and 81972057, the Natural Science Foundation of Shandong Province, grant number ZR2019PH059, and the Soft Science Research Topic of Wuxi Association for Science and Technology, grant number KX-22-C186.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
References
1
BaiX.LvH.ZhangF.LiuJ.FanZ.XuL.et al (2014). Identification of a novel missense mutation in the WFS1 gene as a cause of autosomal dominant nonsyndromic sensorineural hearing loss in all-frequencies.Am. J. Med. Genet. Part A164A3052–3060. 10.1002/ajmg.a.36760
2
BaiX.ZhangC.ZhangF.XiaoY.JinY.WangH.et al (2020). Five novel mutations in LOXHD1 gene were identified to cause autosomal recessive nonsyndromic hearing loss in four Chinese families.Biomed Res. Int.2020:1685974. 10.1155/2020/1685974
3
BandmannO.BurtonE. A. (2010). Genetic Zebrafish models of neurodegenerative diseases.Neurobiol. Dis.4058–65. 10.1016/j.nbd.2010.05.017
4
BathinaS.DasU. N. (2015). Brain-derived neurotrophic factor and its clinical implications.Arch. Med. Sci.111164–1178. 10.5114/aoms.2015.56342
5
BillB. R.PetzoldA. M.ClarkK. J.SchimmentiL. A.EkkerS. C. (2009). A primer for morpholino use in Zebrafish.Zebrafish669–77. 10.1089/zeb.2008.0555
6
CampbellW. A.YangH.ZetterbergH.BaulacS.SearsJ. A.LiuT.et al (2006). Zebrafish lacking alzheimer presenilin enhancer 2 (Pen-2) demonstrate excessive p53-dependent apoptosis and neuronal loss.J. Neurochem.961423–1440. 10.1111/j.1471-4159.2006.03648.x
7
ChenP.ZindyF.AbdalaC.LiuF.LiX.RousselM. F.et al (2003). Progressive hearing loss in mice lacking the cyclin-dependent kinase inhibitor Ink4d.Nat. Cell Biol.5422–426. 10.1038/ncb976
8
CoffinA. B.RamcharitarJ. (2016). Chemical ototoxicity of the fish inner ear and lateral line.Adv. Exp. Med. Biol.877419–437.
9
CunhaC.BrambillaR.ThomasK. L. (2010). A simple role for BDNF in learning and memory?Front. Mol. Neurosci.3:1. 10.3389/neuro.02.001.2010
10
DasS.RajanikantG. K. (2014). Huntington disease: Can a Zebrafish trail leave more than a ripple?Neurosci. Biobehav. Rev.45258–261. 10.1016/j.neubiorev.2014.06.013
11
De RoseR. A.FarberS. A. (2003). Retraction for Smart et al., Annexin 2-caveolin 1 complex is a target of ezetimibe and regulates intestinal cholesterol transport.Proc. Natl. Acad. Sci. U.S.A.110:797. 10.1073/pnas.0400441101
12
DownesG. B.GranatoM. (2004). Acetylcholinesterase function is dispensable for sensory neurite growth but is critical for neuromuscular synapse stability.Dev. Biol.270232–245. 10.1016/j.ydbio.2004.02.027
13
FournierN. M.LeeB.BanasrM.ElsayedM.DumanR. S. (2012). Vascular endothelial growth factor regulates adult hippocampal cell proliferation through MEK/ERK- and PI3K/Akt-dependent signaling.Neuropharmacology63642–652. 10.1016/j.neuropharm.2012.04.033
14
GaoS.ChengC.WangM.JiangP.ZhangL.WangY.et al (2020). Blebbistatin inhibits neomycin-induced apoptosis in hair cell-like HEI-OC-1 cells and in cochlear hair cells.Front. Cell. Neurosci.13:590. 10.3389/fncel.2019.00590
15
GermanaA.GuerreraM. C.LauraR.LevantiM.AragonaM.MhalhelK.et al (2020). Expression and localization of BDNF/TrkB system in the Zebrafish inner ear.Int. J. Mol. Sci.21:5787. 10.3390/ijms21165787
16
GongJ.WangX.ZhuC.DongX.ZhangQ.WangX.et al (2017). Insm1a regulates motor neuron development in Zebrafish.Front. Mol. Neurosci.10:274. 10.3389/fnmol.2017.00274
17
GrilletN.SchwanderM.HildebrandM. S.SczanieckaA.KolatkarA.VelascoJ.et al (2009). Mutations in LOXHD1, an evolutionarily conserved stereociliary protein, disrupt hair cell function in mice and cause progressive hearing loss in humans.Am. J. Hum. Genet.85328–337. 10.1016/j.ajhg.2009.07.017
18
HeZ. H.LiM.FangQ. J.LiaoF. L.ZouS. Y.WuX.et al (2021). Foxg1 promotes aging inner ear hair cell survival through activation of the autophagy pathway.Autophagy174341–4362. 10.1080/15548627.2021.1916194
19
HuangY.WangX.WangX.XuM.LiuM.LiuD.et al (2013). Nonmuscle myosin II-B (myh10) expression analysis during Zebrafish embryonic development.Gene Expr. Patterns13265–270. 10.1016/j.gep.2013.04.005
20
HungG. Y.WuC. L.ChouY. L.ChienC. T.HorngJ. L.LinL. Y.et al (2019). Cisplatin exposure impairs ionocytes and hair cells in the skin of Zebrafish embryos.Aquat. Toxicol.209168–177. 10.1016/j.aquatox.2019.02.006
21
KaiserD. M.AcharyaM.LeightonP. L.WangH.DaudeN.WohlgemuthS.et al (2012). Amyloid beta precursor protein and prion protein have a conserved interaction affecting cell adhesion and CNS development.PLoS One7:e51305. 10.1371/journal.pone.0051305
22
KempfleJ. S.DuroM. V.ZhangA.AmadorC. D.KuangR.LuR.et al (2021). A novel small molecule neurotrophin-3 analogue promotes inner ear neurite outgrowth and synaptogenesis In vitro.Front. Cell. Neurosci.15:666706. 10.3389/fncel.2021.666706
23
KohashiT.OdaY. (2008). Initiation of Mauthner- or non-Mauthner-mediated fast escape evoked by different modes of sensory input.J. Neurosci.2810641–10653. 10.1523/JNEUROSCI.1435-08.2008
24
LeitnerM. G. (2014). Zebrafish in auditory research: Are fish better than mice?J. Physiol.5924611–4612. 10.1113/jphysiol.2014.280438
25
LiA.YouD.LiW.CuiY.HeY.LiW.et al (2018). Novel compounds protect auditory hair cells against gentamycin-induced apoptosis by maintaining the expression level of H3K4me2.Drug Deliv.251033–1043. 10.1080/10717544.2018.1461277
26
LiuB. B.LuoL.LiuX. L.GengD.LiuQ.YiL. T.et al (2015). 7-Chlorokynurenic acid (7-CTKA) produces rapid antidepressant-like effects: Through regulating hippocampal microRNA expressions involved in TrkB-ERK/Akt signaling pathways in mice exposed to chronic unpredictable mild stress.Psychopharmacology232541–550. 10.1007/s00213-014-3690-3
27
LiuL.ChenY.QiJ.ZhangY.HeY.NiW.et al (2016). Wnt activation protects against neomycin-induced hair cell damage in the mouse cochlea.Cell Death Dis.7:e2136. 10.1038/cddis.2016.35
28
MacRaeC. A.PetersonR. T. (2015). Zebrafish as tools for drug discovery.Nat. Rev. Drug Discov.14721–731. 10.1038/nrd4627
29
MarchettaP.SavitskaD.KublerA.AsolaG.MantheyM.MöhrleD.et al (2020). Age-dependent auditory processing deficits after cochlear synaptopathy depend on auditory nerve latency and the ability of the brain to recruit LTP/BDNF.Brain Sci.10:710. 10.3390/brainsci10100710
30
MeshalkinaD. A.SongC.KalueffA. V. (2017). Better lab animal models for translational neuroscience research and CNS drug development.Lab Anim.4691–92. 10.1038/laban.1236
31
NaseviciusA.EkkerS. C. (2000). Effective targeted gene ‘knockdown’ in Zebrafish.Nat. Genet.26216–220. 10.1038/79951
32
PangJ.HouJ.ZhouZ.RenM.MoY.YangG.et al (2020). Safflower yellow improves synaptic plasticity in APP/PS1 mice by regulating microglia activation phenotypes and BDNF/TrkB/ERK signaling pathway.Neuromolecular Med.22341–358. 10.1007/s12017-020-08591-6
33
SegalR. A. (2003). Selectivity in neurotrophin signaling: Theme and variations.Annu. Rev. Neurosci.26299–330. 10.1146/annurev.neuro.26.041002.131421
34
SelcherJ. C.WeeberE. J.VargaA. W.SweattJ. D.SwankM. (2002). Protein kinase signal transduction cascades in mammalian associative conditioning.Neuroscientist8122–131. 10.1177/107385840200800208
35
SheffieldA. M.SmithR. J. H. (2018). The epidemiology of deafness.Cold Spring Harb. Perspect. Med.[Epub ahead of print]. 10.1101/cshperspect.a033258
36
SlatteryE. L.WarcholM. E. (2010). Cisplatin ototoxicity blocks sensory regeneration in the avian inner ear.J. Neurosci.303473–3481. 10.1523/JNEUROSCI.4316-09.2010
37
Sloan-HeggenC. M.BiererA. O.ShearerA. E.KolbeD. L.NishimuraC. J.FreesK. L.et al (2016). Comprehensive genetic testing in the clinical evaluation of 1119 patients with hearing loss.Hum. Genet.135441–450. 10.1007/s00439-016-1648-8
38
StawickiT. M.EsterbergR.HaileyD. W.RaibleD. W.RubelE. W. (2015). Using the Zebrafish lateral line to uncover novel mechanisms of action and prevention in drug-induced hair cell death.Front. Cell. Neurosci.9:46. 10.3389/fncel.2015.00046
39
StewartA. M.UllmannJ. F.NortonW. H.ParkerM. O.BrennanC. H.GerlaiR.et al (2015). Molecular psychiatry of Zebrafish.Mol. Psychiatry202–17. 10.1038/mp.2014.128
40
Stooke-VaughanG. A.ObholzerN. D.BaxendaleS.MegasonS. G.WhitfieldT. T. (2015). Otolith tethering in the Zebrafish otic vesicle requires otogelin and alpha-tectorin.Development1421137–1145. 10.1242/dev.116632
41
SummertonJ. (1999). Morpholino antisense oligomers: The case for an RNASE H-independent structural type.Biochim. Biophys. Acta1489141–158. 10.1016/s0167-4781(99)00150-5
42
SummertonJ.WellerD. (1997). Morpholino antisense oligomers: Design, preparation, and properties.Antisense Nucleic Acid Drug Dev.7187–195. 10.1089/oli.1.1997.7.187
43
SweattJ. D. (2001). The neuronal Map kinase cascade: A biochemical signal integration system subserving synaptic plasticity and memory.J. Neurochem.761–10. 10.1046/j.1471-4159.2001.00054.x
44
VinkH. A.VersnelH.KroonS.KlisS. F. L.RamekersD. (2021). BDNF-mediated preservation of spiral ganglion cell peripheral processes and axons in comparison to that of their cell bodies.Hear. Res.400:108114. 10.1016/j.heares.2020.108114
45
WangC.ZhongZ.SunP.ZhongH.LiH.ChenF.et al (2017). Evaluation of the hair cell regeneration in Zebrafish larvae by measuring and quantifying the startle responses.Neural Plast.2017:8283075. 10.1155/2017/8283075
46
WeiG.ZhangX.CaiC.ShengJ.XuM.WangC.et al (2022). Dual-specificity phosphatase 14 regulates Zebrafish hair cell formation through activation of p38 signaling pathway.Front. Cell. Neurosci.16:840143. 10.3389/fncel.2022.840143
47
WilsonB. S.TucciD. L.MersonM. H.O’DonoghueG. M. (2017). Global hearing health care: New findings and perspectives.Lancet3902503–2515. 10.1016/S0140-6736(17)31073-5
48
WyllieA. H. (1980). Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation.Nature284555–556. 10.1038/284555a0
49
YanT.HeB.WanS.XuM.YangH.XiaoF.et al (2017). Antidepressant-like effects and cognitive enhancement of Schisandra chinensis in chronic unpredictable mild stress mice and its related mechanism.Sci. Rep.7:6903. 10.1038/s41598-017-07407-1
50
YangR. M.TaoJ.ZhanM.YuanH.WangH. H.ChenS. J.et al (2019). TAMM41 is required for heart valve differentiation via regulation of PINK-PARK2 dependent mitophagy.Cell Death Differ.262430–2446. 10.1038/s41418-019-0311-z
51
YiL. T.LiJ.LiuB. B.LuoL.LiuQ.GengD.et al (2014). BDNF-ERK-CREB signalling mediates the role of miR-132 in the regulation of the effects of oleanolic acid in male mice.J. Psychiatry Neurosci.39348–359. 10.1503/jpn.130169
52
ZhangJ.QiJ.WuS.PengL.ShiY.YangJ.et al (2017). Fatty acid binding protein 11a is required for brain vessel integrity in Zebrafish.Front. Physiol.8:214. 10.3389/fphys.2017.00214
53
ZhangY.LiW.HeZ.WangY.ShaoB.ChengC.et al (2019). Pre-treatment with fasudil prevents neomycin-induced hair cell damage by reducing the accumulation of reactive oxygen species.Front. Mol. Neurosci.12:264. 10.3389/fnmol.2019.00264
54
ZhongZ.FuX.LiH.ChenJ.WangM.GaoS.et al (2020). Citicoline protects auditory hair cells against neomycin-induced damage.Front. Cell Dev. Biol.8:712. 10.3389/fcell.2020.00712
Summary
Keywords
zebrafish, hair cell, Loxhd1b, BDNF/TrkB/ERK signaling pathway, morpholino
Citation
Liu J, Zhang X, Zhang Q, Wang R, Ma J, Bai X and Wang D (2022) Loxhd1b inhibits the hair cell development in zebrafish: Possible relation to the BDNF/TrkB/ERK pathway. Front. Cell. Neurosci. 16:1065309. doi: 10.3389/fncel.2022.1065309
Received
09 October 2022
Accepted
08 November 2022
Published
24 November 2022
Volume
16 - 2022
Edited by
Chen Chen, Stanford University, United States
Reviewed by
Fengjie Sun, Georgia Gwinnett College, United States; Miren Revuelta, University of the Basque Country, Spain; Jing Chen, University of Warwick, United Kingdom
Updates
Copyright
© 2022 Liu, Zhang, Zhang, Wang, Ma, Bai and Wang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Dawei Wang, wangdawei@sdu.edu.cn
This article was submitted to Non-Neuronal Cells, a section of the journal Frontiers in Cellular Neuroscience
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.