Lauren B. Coco
Caren L. Freel Meyers- Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, United States
A Correction on
An activity-based probe for antimicrobial target DXP synthase, a thiamin diphosphate-dependent enzyme
by Coco LB and Freel Meyers CL (2024). Front. Chem. Biol 3:1389620. doi: 10.3389/fchbi.2024.1389620
In the published article, there was an error. The concentrations of click reaction components were inadvertently reported as 10-fold higher than actually used for labeling experiments using purified recombinant DXPS.
A correction has been made to “4.4 General protocol for DXPS labeling by 1”. This sentence previously stated:
“Solutions were cooled to ambient temperature, and a 7.5x CuAAC reaction stock (4 uL, prepared immediately prior to addition) was added to achieve final concentrations of 1 mM tris ((1-benzyl-4-triazolyl)methyl)amine (TBTA), 10 mM CuSO4, and 10 mM tris(2-carboxyethyl)phosphine (TCEP).”
The corrected sentence appears below:
“Solutions were cooled to ambient temperature, and a 7.5x CuAAC reaction stock (4 uL, prepared immediately prior to addition) was added to achieve final concentrations of 0.1 mM tris ((1-benzyl-4-triazolyl)methyl)amine (TBTA), 1 mM CuSO4, and 1 mM tris(2-carboxyethyl)phosphine (TCEP).”
In the published article, there was an error. The concentrations of click reaction components were inadvertently reported as 10-fold higher than actually used in experiments to label DXPS in bacterial lysate.
A correction has been made to “4.6 Detection of DXPS in complex bacterial lysate”, subsection “4.6.2 Labeling of DXPS by 1 in lysate”, paragraph 1. This sentence previously stated:
“A 7.5× CuAAC reaction stock (1 mM TBTA, 10 mM CuSO4, and 10 mM TCEP) was prepared and 4 μL was added to each sample followed by addition of TAMRA-azide (6 μL of a 5 mM stock in DMSO).”
The corrected sentence appears below:
“A 7.5× CuAAC reaction stock was prepared and 4 μL was added to each sample (to achieve final concentrations of 0.1 mM TBTA, 1 mM CuSO4, and 1 mM TCEP), followed by addition of TAMRA-azide (6 μL of a 5 mM stock in DMSO).”
The original article has been updated.
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Keywords: 1-deoxy-D-xylulose 5-phosphate synthase, activity-based probe, alkyl acetylphosphonate, thiamin-dependent enzyme, antimicrobial target, vitamin biosynthesis, isoprenoid biosynthesis, bacterial central metabolism
Citation: Coco LB and Freel Meyers CL (2025) Correction: An activity-based probe for antimicrobial target DXP synthase, a thiamin diphosphate-dependent enzyme. Front. Chem. Biol. 4:1670585. doi: 10.3389/fchbi.2025.1670585
Received: 21 July 2025; Accepted: 09 September 2025;
Published: 19 September 2025.
Edited by:
Virginia Rodríguez Robledo, University of Castilla-La Mancha, SpainReviewed by:
Flora Cruz-López, Universidad Autónoma de Nuevo León, MexicoCopyright © 2025 Coco and Freel Meyers. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Caren L. Freel Meyers, Y21leWVyc0BqaG1pLmVkdQ==