ORIGINAL RESEARCH article
Front. Fungal Biol.
Sec. Fungal Biotechnology
Volume 6 - 2025 | doi: 10.3389/ffunb.2025.1617458
Lignocellulose adaptation drives polysaccharide biosynthesis in Tremella fuciformis: Metabolomic and proteomic insights into CAZyme regulation
Provisionally accepted
- 1Sichuan Academy of Agricultural Sciences, Chengdu, China
- 2Sichuan Institute of Edible Fungi, Chengdu, China
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Background/Objectives: T. fuciformis is an edible fungus prized for its culinary value. The polysaccharide content of T. fuciformis grown on a Cyclobalanopsis substrate (TY3) was significantly higher than those grown on a mixed substrate (TF1) made of wheat bran and cottonseed hull. Methods: Metabolomics and proteomics were used to assess the effects of the lignocellulose (consists of cellulose, hemicellulose and lignin) in different growth substrates on the polysaccharide content of Tremella fuciformis and its formation mechanism. Results: TY3 had a higher lignocellulose content than TF1. The metabolites of carbohydrates and carbohydrate conjugates in TY3-grown specimens were significantly up-regulated. Among 21 metabolic pathways identify proteins enriched, carbohydrate metabolism was the most enriched. The Carbohydrate-Active enzyme (CAZyme) database was used to annotate 161 carbohydrate enzymes, and 67 of them were differentially expressed proteins. Carbohydrate synthetases were up-regulated much more using TY3. Conclusions: T. fuciformis grown on TY3 was verified to possess a lower ability of lignocellulose degradation (evinced by decreases of cellulase, xylanase, and lignin peroxidase synthesis), but stronger ability of carbohydrate synthesis (evinced by increases of cellulose and hemicellulose synthesis). Our study enhances the control of polysaccharide content in T. fuciformis, thereby facilitating its processing for food applications.
Keywords: Tremella fuciformis, lignocellulose in growth substrates, Polysaccharide content, CAZyme, Metabolomic and proteomic insights
Received: 29 Apr 2025; Accepted: 09 Jun 2025.
Copyright: © 2025 Peng, Xu, Dong, Zhang, Xie, Zhang, Shu and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Weihong Peng, Sichuan Academy of Agricultural Sciences, Chengdu, China
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