ORIGINAL RESEARCH article
Front. Fungal Biol.
Sec. Fungi-Plant Interactions
Volume 6 - 2025 | doi: 10.3389/ffunb.2025.1618728
This article is part of the Research TopicPlant-Friendly Microorganisms as a Bio-Barrier Against PathogensView all 3 articles
Biocontrol potential of Trichoderma derived chitinase: Optimization, purification and antifungal activity against soil borne pathogens of apple
Provisionally accepted
- Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, India
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Apple is most important fruit crop of Himachal Pradesh, contributing substantially to the state's economy. However, soil borne diseases have emerged as a major concern affecting nursery raising of apple. Trichoderma species produces chitinase, an enzyme that degrade chitin, a major component of fungal cell wall. This study aimed to optimize growth parameters for chitinase production, their extraction, purification, characterization and assessing the antifungal potential against soil borne pathogens of apple. Fourteen isolates of Trichoderma spp. produced chitinase in colloidal chitin agar medium at varying extent. The optimal incubation period, pH, substrate concentration and incubation temperature was 7 days, 5, 1 per cent and 30°C, respectively. Whereas, thermal and pH stability ranged from 30 to 50°C and 4 to 6, respectively. Chitinases were purified from T. atroviride UHFTA005, UHFTA006 and T. virens UHFTV017 with molecular mass of 40 KDa. Chitinase from T. atroviride UHFTA005 at 0.60 μL inhibited in vitro growth of Dematophora necatrix (92.22%) and Sclerotium rolfsii (91.11%). Further in vivo evaluation of chitinase, T. atroviride UHFTA005 found to be more effective against white root rot and seedling blight of apple with 86.67 and 73.33 per cent disease control, respectively and with 86.67 per cent disease control of white root rot in nursery field conditions.
Keywords: biological control, Chitin, Chitinase, Soil borne, Trichoderma
Received: 26 Apr 2025; Accepted: 16 Jun 2025.
Copyright: © 2025 Walia, Sharma, Gupta, Rana, Sharma and Verma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Satish Kumar Sharma, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, India
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.