In the original article, there was a mistake in Table 1 as published. We misplaced the forward primer and reverse primer when typesetting, which has been adjusted. The corrected Table 1 appears below.
Table 1
| Primer | Primer sequences (5′-3′) | |
|---|---|---|
| Gapdh | F: TGATGGGTGTGAACCACGAG | R: TCAGTGTAGCCCAAGATGCC |
| U6 | F: CTCGCTTCGGCAGCACA | R: AACGTTCACGAATTTGCGT |
| Bcl-xl | F: CTGAATCGGAGATGGAGACC | R: TGGGATGTCAGGTCACTGAA |
| Hdac3 | F: CACCCTATGAAGCCCCATCG | R: GAGACCGTAATGCAGGACCAG |
| miR-296-5p | F: CGACGAGGGCCCCCCCT | R: GTATCCAGTGCAGG GTCCGA |
Primer sequences used in RT-qPCR analysis.
RT-qPCR, reverse transcription-quantitative polymerase chain reaction; Gapdh, glyceraldehyde-3-phosphate dehydrogenase; Bcl-xl, B-cell leukemia-XL; Hdac3, histone deacetylase 3; miR-296-5p, microRNA-296-5p; F, forward; R, reverse.
Additionally, there was also a mistake in the text of the published article. We are sorry that the previous method description was not clear enough. Now we have modified the related method description: “For reverse transcription (RT) of mRNA, 1 μg of RNA was synthesized into cDNA at 42°C for 50 min by using the TaqMan RT reagent (Roche, Canton of Basel, Switzerland). For the RT of miRNA, specific stem-loop primers were used to synthesize cDNA.”
A correction has been made to Methods, RNA Isolation and Quantitation, Paragraph 1:
Total RNA from tissues or cells was extracted using the miRNeasy Mini kit (QIAGEN, GmbH, Hilden, Germany) and subsequently quantified using NanoDrop ND-1000 Spectrophotometer (NanoDrop Products, Wilmington, DE, USA), whereas RNA integrity was evaluated by microfluidic electrophoresis. For reverse transcription (RT) of mRNA, 1 μg of RNA was synthesized into cDNA at 42°C for 50 min by using the TaqMan RT reagent (Roche, Canton of Basel, Switzerland). For the RT of miRNA, specific stem-loop primers were used to synthesize cDNA.
The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Publisher's Note
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Summary
Keywords
histone deacetylase 3, microRNA-296-5p, B-cell leukemia-XL, type 1 diabetes mellitus, peripheral blood mononuclear cells, apoptosis
Citation
Hu Q, Che G, Yang Y, Xie H and Tian J (2021) Corrigendum: Histone Deacetylase 3 Aggravates Type 1 Diabetes Mellitus by Inhibiting Lymphocyte Apoptosis Through the microRNA-296-5p/Bcl-xl Axis. Front. Genet. 12:715061. doi: 10.3389/fgene.2021.715061
Received
26 May 2021
Accepted
24 August 2021
Published
22 September 2021
Volume
12 - 2021
Edited and reviewed by
Peter G. Zaphiropoulos, Karolinska Institutet (KI), Sweden
Updates
Copyright
© 2021 Hu, Che, Yang, Xie and Tian.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jing Tian xiaojing800120@jlu.edu.cn
This article was submitted to RNA, a section of the journal Frontiers in Genetics
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.