Fertilization and early development are processes characterized by delicate genetic and epigenetic regulation. In brief, sperm fuses with the oocyte to form a zygote, which then undergoes zygotic genome activation (ZGA) and lineage specification, ultimately implanting during the blastocyst stage. This process is intricately regulated by numerous key regulatory genes and critical epigenetic modifications, which are vital for the post-implantation development and subsequent pregnancy. This Research Topic comprises three original studies, one case report, and two reviews, which will advance our understanding of genetics and epigenetics in the fertilization and early embryonic development.
Germ cell maturation and embryo development require the precise gene expression program at different developmental stage, which sculpts the dynamic epigenetic modification landscape. Polycomb group (PcG) complex, with its two components PRC1 and PRC2, catalyzes the H2AK119ub1 and H3K27me3, which regulate cell fate by repressing gene expression. Li et al. reviewed the role of the PcG complex in mammalian development, contributing to our understanding of multifaceted functions of the PcG complex and H3K27me3. Additionally, H3K27me3 may be responsible for marking recurrent double-strand breaks (DSBs) in transgenerational DNA repair. Due to the absence of sister chromatids, the genome of haploid round spermatids cannot repair DSBs through homologous recombination repair (). How spermatids cope with this vulnerable genome state remains not fully elucidated. Scheuren et al. focused on the distribution of DSBs in human sperm, revealing a strong colocalization between H3K27me3 and recurrent DSBs. This result suggests the paternal H3K27me3 may serve as a guiding marker for maternal Polθ in the zygote to execute transgenerational DNA repair at sites of recurrent DSBs.
Following meiosis, round spermatids undergo intricate morphological changes to form mature spermatozoa. Abnormal sperm structures could prevent sperm from approaching or fusing with the oocyte, which is a key cause of fertilization failure. With the application of whole-genome sequencing, many genes associated with teratozoospermia have been identified. Bragina et al. analyzed samples from 12 globozoospermia patients and detected homozygous variants in Dpy19l2 and Spata16 in three of these cases. Mutations in Dpy19l2 and Spata16 have been identified as key causes of globozoospermia (; ). Moreover, globozoospermia phenotype has also been validated in mouse with Dpy19l2 or Spata16 deficiency (; ). Genetically engineered mouse models offer a pathway for studying the mechanisms of genetic diseases. However, because of the complexity of gene mutation and homology differences, mouse models may not always accurately mimic human phenotypes. Nguyen et al. conducted loss-of-function studies in mouse model on 13 testis-enriched genes including Adam20 (A gene associated with fertilization failure in human), demonstrating that these genes are not essential for male fertility in mice. Therefore, we should cautiously evaluate the results derived from mouse models.
Currently, assisted reproductive technologies are widely used to address fertilization failure and to prevent the transmission of pathogenic parental genes to offspring (). Hu et al. firstly reported a woman with Hereditary Leiomyomatosis and Renal Cell Cancer syndrome who successfully delivered a healthy baby by preimplantation genetic testing for monogenic disorders (PGT-M). This case highlights the potential of PGT-M in addressing the reproductive needs of patients with genetic diseases. Apart from genetic defects, environmental pollutants can indirectly impair fertility by affecting gene expression and epigenetics (). Wang et al. discussed the reproductive toxicity of endocrine-disrupting chemicals (EDCs) in female reproduction, summarizing the current epidemiological studies and animal model studies for five major EDCs. Environmental pollutants can induce epigenetic alterations through oxidative stress and DNA damage, leading to impaired gene regulation and organelles dysfunction in germ cell or embryo (; ). Therefore, healthy populations without genetic defects should also pay attention to the potential reproductive risks posed by environmental pollution.
Finally, we thank all authors for their contributions in the Research Topic. This Research Topic focuses on the genetic and epigenetic regulation of fertilization and early development, a field that has rapidly advanced over the recent years. While the scope of this Research Topic is limited, the progress it covers is exhilarating, as the advances in basic science are indeed contributing to the disease prevention and clinical intervention in reproductive medicine.
Statements
Author contributions
YT: Writing–original draft. Y-fW: Writing–original draft. Y-lM: Writing–review and editing. L-qZ: Writing–review and editing.
Funding
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by National Natural Science Foundation of China (32170820).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
References
1
BrezinaP. R.KuttehW. H. (2015). Clinical applications of preimplantation genetic testing. Bmj350, g7611. 10.1136/bmj.g7611
2
CastanedaJ. M.ShimadaK.SatouhY.YuZ.DevlinD. J.IkawaM.et al (2021). FAM209 associates with DPY19L2, and is required for sperm acrosome biogenesis and fertility in mice. J. Cell Sci.134, jcs259206. 10.1242/jcs.259206
3
DamA. H.KoscinskiI.KremerJ. A.MoutouC.JaegerA. S.OudakkerA. R.et al (2007). Homozygous mutation in SPATA16 is associated with male infertility in human globozoospermia. Am. J. Hum. Genet.81, 813–820. 10.1086/521314
4
FujiharaY.OjiA.LarasatiT.Kojima-KitaK.IkawaM. (2017). Human globozoospermia-related gene Spata16 is required for sperm formation revealed by CRISPR/Cas9-Mediated mouse models. Int. J. Mol. Sci.18, 2208. 10.3390/ijms18102208
5
GreenM. P.HarveyA. J.FingerB. J.TarulliG. A. (2021). Endocrine disrupting chemicals: impacts on human fertility and fecundity during the peri-conception period. Environ. Res.194, 110694. 10.1016/j.envres.2020.110694
6
KitaokaM.YamashitaY. M. (2024). Running the gauntlet: challenges to genome integrity in spermiogenesis. Nucleus15, 2339220. 10.1080/19491034.2024.2339220
7
KoscinskiI.ElinatiE.FossardC.RedinC.MullerJ.Velez de La CalleJ.et al (2011). DPY19L2 deletion as a major cause of globozoospermia. Am. J. Hum. Genet.88, 344–350. 10.1016/j.ajhg.2011.01.018
8
Lopez-RodriguezD.FranssenD.BakkerJ.LomnicziA.ParentA. S. (2021). Cellular and molecular features of EDC exposure: consequences for the GnRH network. Nat. Rev. Endocrinol.17, 83–96. 10.1038/s41574-020-00436-3
9
StrazzulloM.MatarazzoM. R. (2017). Epigenetic effects of environmental chemicals on reproductive biology. Curr. Drug Targets18, 1116–1124. 10.2174/1389450117666161025100125
Summary
Keywords
germ cell, fertilization, early development, genetics, epigenetics
Citation
Tian Y, Wang Y, Miao Y and Zhou L (2024) Editorial: Fertilization and early development: genetics and epigenetics. Front. Genet. 15:1465510. doi: 10.3389/fgene.2024.1465510
Received
16 July 2024
Accepted
23 July 2024
Published
30 July 2024
Volume
15 - 2024
Edited and reviewed by
Michael E. Symonds, University of Nottingham, United Kingdom
Updates
Copyright
© 2024 Tian, Wang, Miao and Zhou.
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*Correspondence: Yi-liang Miao, miaoyl@mail.hzau.edu.cn; Li-quan Zhou, zhouliquan@hust.edu.cn
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.