ORIGINAL RESEARCH article
Front. Genet.
Sec. Genomics of Plants and the Phytoecosystem
Volume 16 - 2025 | doi: 10.3389/fgene.2025.1598523
This article is part of the Research TopicFostering the Next Generation Crop: Systems and Synthetic Biology PerspectiveView all articles
Elucidating the role of Pyrabactin-Like Receptors of finger millet under drought and salinity stress: an insight into in-silico, machine learning and molecular approaches
Provisionally accepted
- 1Sandip University, Nashik, Maharashtra, India
- 2Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, India
- 3Rajagiri College of Social Sciences, Kochi, Kerala, India
- 4ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand, India
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Finger millet (Eleusine coracana (L.) Gaertn) is a nutraceutical crop with considerable potential to enhance food and nutritional security. It has a comparatively higher tolerance for abiotic stresses; nonetheless, the receptor protein implicated in its stress signalling pathways remains inadequately understood. The Pyrabactin Resistance 1-like (PYL) receptors constitute a protein family in plants that are crucial for abscisic acid (ABA) signalling and assist plants in managing abiotic stressors. Using bioinformatics, machine learning, and molecular approaches, identification, characterization, and expression profiling of PYL receptors in response to drought and salt stress have been studied. The finger millet genome revealed 14 PYL genes, irregularly dispersed over four of the nine mapped on chromosomes. The phylogenetic analysis delineated the classification of PYL gene family members in finger millet into three subfamilies. Moreover, the PYL genes of finger millet possess several cis-regulatory elements associated with plant development and growth, including the regulation of transcription, hormone signalling, and stress responses. Utilizing machine learning approach, five putative PYL genes (EcPYL4-2A, EcPYL7-2B, EcPYL11-5A, EcPYL12-5A, and EcPYL14-5B) exhibited expression levels above 70% in response to drought and salt stress. These genes were further validated with qRT-PCR expression profiling. These genes were found to be expressed under salt and drought conditions; however, their expression levels varied across tissues and genes. As a result of this study, suitable candidate genes (EcPYL7-2B and EcPYL12-5A) for drought and salinity stress could be selected for further characterization through genome editing and other molecular methods.
Keywords: ABA receptor, ABA signaling pathways, abiotic stress, finger millet, PYL gene family
Received: 23 Mar 2025; Accepted: 14 May 2025.
Copyright: © 2025 Rani, Theivanayagam, Singh, Joshi, Gupta and Yadav. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Dinesh Joshi, ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, 263601, Uttarakhand, India
Dinesh Yadav, Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, India
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