ORIGINAL RESEARCH article
Front. Genet.
Sec. Genomics of Plants and the Phytoecosystem
Volume 16 - 2025 | doi: 10.3389/fgene.2025.1629260
This article is part of the Research TopicStrategies for Crops to Confront Extreme Weather and Pests/DiseasesView all articles
A xyloglucan endotransglucosylase/hydrolase gene, IbXTH16, increases cold tolerance in transgenic sweetpotato
Provisionally accepted
- Liaoning Academy of Agricultural Sciences, Shenyang, China
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Introduction: Low temperature is a key environmental factor that threaten sweetpotato growth and development. In-depth studies on the gene functions underlying cold resistance are important for genetic engineering in sweetpotato.The IbXTH16 gene was cloned using a homologous cloning approach. Its expression was detected in sweetpotato leaves subjected to low-temperature stress and brassinosteroid treatment. Subsequently, the IbXTH16 gene was introduced into sweetpotato variety Lizixiang to generate IbXTH16-overexpressing plants, thereby enabling the functional validation of the IbXTH16.: The IbXTH16 gene was cloned from the cold-tolerant variety LHS21. Its 879 bp coding sequence encoded a 292 aa protein with a molecular weight of 32.983 kDa and a pI of 8.47. The 2039 bp genomic sequence of IbXTH16 contained two exons and one intron. The IbXTH16 protein was localized in the cell membrane.IbXTH16 was strongly induced by 4℃ and brassinosteroid. IbXTH16 positively regulates cold tolerance of sweetpotato by activating the BR and proline pathways.
Keywords: Sweetpotato, cold tolerance, IbXTH16, BR, Proline
Received: 15 May 2025; Accepted: 09 Jun 2025.
Copyright: © 2025 Yu, Pan, Liu, Yang and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Zhenlei Liu, Liaoning Academy of Agricultural Sciences, Shenyang, China
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