ORIGINAL RESEARCH article
Front. Mar. Sci.
Sec. Marine Biotechnology and Bioproducts
Volume 12 - 2025 | doi: 10.3389/fmars.2025.1588772
Complete genome sequencing and antibiofilm activity of an endophytic bacterium associated with marine sponge Hyrtios erectus collected from the Red Sea
Provisionally accepted
- 1King Abdulaziz University, Jeddah, Saudi Arabia
- 2Kaduna State University, Kaduna, Nigeria
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Genome sequences of sponge bacterial endophytes will be useful for understanding the bioactive compound synthetic potential and molecular mechanisms of sponge-bacteria interactions. In this study, the complete genome of the bacterium E9 isolated from the Red Sea sponge species was sequenced and its antibiofilm activity was assessed through laboratory assay. Experiments indicated the strong antibacterial and antibiofilm activity of the extracts of bacterium E9. Complete genome sequencing reveals that genome assembly generated a single chromosome of 2123451 base pairs with a guaninecytosine (GC) content of 32.9 % with 2420 protein coding sequences and a gene/genome ratio of 83.7 %. The bacterial strain was identified as Staphylococcus epidermidis based on phylogenetic analysis.A total of 9 biosynthetic gene clusters were identified in the genome using the open-source platform AntiSMASH. The observed antibacterial and antibiofilm activity of the strain E9 may be due the presence of gene clusters such as nonribosomal peptides, lasso peptides and terpenes. Overall, the whole genome analysis indicated the bacterium's capability to adapt in diverse environments including invertebrate hosts, and bioactive compound synthesis.
Keywords: Sponge-associated bacteria, Bioactive metabolites, Antibacterial compounds, complete genome, Biofilms, Biosynthetic gene cluster
Received: 06 Mar 2025; Accepted: 21 Apr 2025.
Copyright: © 2025 Jamal, Abdurrahman, H. Amran, Almatry and Sathianeson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
MAMDOH Jamal, King Abdulaziz University, Jeddah, Saudi Arabia
Satheesh Sathianeson, King Abdulaziz University, Jeddah, Saudi Arabia
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