ORIGINAL RESEARCH article
Front. Mar. Sci.
Sec. Marine Biology
Volume 12 - 2025 | doi: 10.3389/fmars.2025.1635506
Nitric oxide combined with abscisic acid contributes to high-temperature tolerance in macroalga Gracilariopsis lemaneiformis
Provisionally accepted
- Ningbo University, Ningbo, China
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The economic macroalga Gracilariopsis lemaneiformis is widely cultivated in China, yet high temperatures in the summertime constrain its cultivation period on China's southern coasts. Thus, thereby it is of great significance to improve its heat tolerance. Nitric oxide (NO), a small signaling molecule, plays a multifaceted role in resistance to abiotic stresses such as drought, salinity, and low/high temperature. However, the role and underlying mechanism of NO and its interaction with phytohormone abscisic acid (ABA) against high-temperature stress in algae are still unclear. In this study, the NO donor sodium nitroprusside (SNP) promoted the algal growth (1.23-fold), and actual photosynthetic efficiency, yet it and reduced heat dissipation of G. lemaneiformis at 30℃. In addition, several significantly enriched KEGG pathways, including nitrogen metabolism, vitamin B6 metabolism, and glutathione metabolism after SNP application, were revealed by quantitative proteomics after SNP application. Meanwhile, some proteins involved in carbohydrate metabolism and photosynthesis were also differentially expressed in response to exogenous NO. Last, SNP in combination with ABA synergistically acted against heat stress via regulating malondialdehyde and proline contents; moreover, NO might act downstream of ABA, demonstrated by the scavenger and inhibitor application. In summary, a scheme for the mechanism of NO-induced high-temperature tolerance was proposed. The present study will provide valuable insights into understanding the mechanism of NO regulatingregulation and high-temperature tolerance in macroalgae.
Keywords: Gracilariopsis lemaneiformis, Nitric Oxide, high temperature, Abscisic Acid, Proteomics
Received: 26 May 2025; Accepted: 03 Oct 2025.
Copyright: © 2025 Chu, Ying, Li, Liu, Xu and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Xue Sun, sunxue@nbu.edu.cn
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