ORIGINAL RESEARCH article
Front. Mar. Sci.
Sec. Aquatic Microbiology
Volume 12 - 2025 | doi: 10.3389/fmars.2025.1646100
Identifying potential keystone microbes from co-occurrence networks in the Gulf of Alaska
Provisionally accepted- 1University of Alaska Fairbanks, Fairbanks, United States
- 2University of Alaska Anchorage, Anchorage, United States
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The Northern Gulf of Alaska (NGA) is a highly productive and diverse marine ecosystem.Differences in nutrient supply and physical circulation between nearshore and offshore waters in the NGA result in a mosaic of water masses with distinct biogeochemical signatures. We hypothesized that microbial communities in these regions not only differ in composition but also in the ecological interaction networks they support. We used amplicon sequencing of the 16S (V4) and 18S (V9) rRNA genes to characterize the microbial community differences between nearshore, continental shelf, and offshore regions in the NGA in summers 2018-2021. We observed significantly different community assemblages by region (MRPP, p = 0.001), with higher relative abundances and cell counts of heterotrophic bacteria and Synechococcus nearshore, elevated Alphaproteobacteria and SAR11 clades offshore, and greater dinoflagellates and Spirotrich ciliates on the shelf. Co-occurrence networks of operational taxonomic units (OTUs) of prokaryotes and eukaryotes were constructed for each region using statistically significant correlations (Spearman rank >0.8, Bonferroni corrected p < 0.05). Overall, the offshore network had higher centralization (0.331) and density (0.112), indicating higher connectivity and therefore more potential interactions compared to the shelf (0.191, 0.069) and nearshore (0.165, 0.041) networks. The nearshore network was characterized by higher proportions of potentially parasitic taxa such as Cryothecomonas aestivalis, Syndiniales Dino Group I, and MAST-1C and parasitoid bacteria Bdellovibrio and like organisms, suggesting that nearshore conditions may increase parasitoid/predator success through increased contact rates.were identified in all three regions, supporting previous findings that kleptoplasty is likely an important strategy across the NGA. Eukaryotic taxa that had the highest degree centrality across all three regions; P. prolonga and Phaeocystis are known to be mixotrophs, suggesting a role for bacterivory in forging a high number of interactions between protists and bacteria. This study represents the first region-specific co-occurrence network analysis across nearshore to offshore gradients in the NGA. By identifying highly connected taxa and potential trophic strategies, our findings provide new insight into how microbial interactions shape community structure and resilience in a dynamic subarctic ecosystem.
Keywords: Co-occurrence network, keystone microbes, trophic interactions, kleptoplasty, parasitoid, Northern Gulf of Alaska, NGA LTER
Received: 12 Jun 2025; Accepted: 04 Aug 2025.
Copyright: © 2025 Brauner, Cohen, Briggs and Hennon. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Megan Brauner, University of Alaska Fairbanks, Fairbanks, United States
Gwenn M M Hennon, University of Alaska Fairbanks, Fairbanks, United States
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