Marta Gonzalez Cañete1,2*
Ana Rodríguez-Villa Lario1,2
David Vega Díez1,2
María Dolores Vélez Velazquez3
Jose Maria Ricart Vaya1
Alba Gómez Zubiaur1- 1Instituto médico ricart, Madrid, Spain
- 2Department of Dermatology, Hospital Universitario Principe de Asturias, Alcala de Henares, Spain
- 3Department of Pathology, Hospital Universitario Principe de Asturias, Alcala de Henares, Spain
Introduction: Frontal fibrosing alopecia (FFA) may involve the interparietal area; however, histopathologic features in this site remain undefined.
Objective: The objective of the study was to histopathologically characterize inflammatory interparietal involvement in patients with FFA in real-world practice.
Methods: A descriptive, retrospective study enrolled patients with FFA who developed interparietal inflammatory findings. Patients underwent two interparietal biopsies; samples were assessed under a uniform protocol, which included follicular counts, inflammatory infiltrate features, scar tissue, vacuolar degeneration (VD) of the follicular epithelium, epithelial or stromal (E/S) clefting, concentric lamellar fibroplasia (CLF), and sebaceous glands (SGs).
Results: A total of 12 females (mean age: 64.25 years) were included. Following dermatopathologic review, six patients were classified as having crown-area FFA (CA-FFA), four patients as having fibrosing alopecia with a pattern distribution (FAPD), and two patients as having indeterminate cicatricial alopecia. CA-FFA showed greater structural loss with scar tissue and sebaceous gland reduction, whereas FAPD showed a miniaturization-dominant profile and increased catagen/telogen hairs. Patients with a biopsy suggestive of CA-FFA more often had a diffuse clinical pattern, were older, had a longer disease duration, and had approximately twice as much occipital involvement than as those in the FAPD group.
Discussion/Conclusion: Given that FFA produces greater structural disruption than FAPD, our results raise the question of whether these conditions are overlapping entities or part of the same process that has progressed. Further studies in patients with FFA and FAPD are needed to elucidate the role of androgenetic factors in addition to lymphohistiocytic infiltrate and perifollicular lamellar fibrosis.
1 Introduction
Frontal fibrosing alopecia (FFA) is an acquired lymphocytic scarring alopecia of increasing incidence worldwide. It is clinically characterized by a progressive frontotemporal hairline recession, often accompanied by eyebrow alopecia. Occasionally, FFA may present with interparietal involvement, a feature that Kossard already reported in 1994 (1). This involvement may reflect coexistence of FFA with androgenetic alopecia (AGA) (2), fibrosing alopecia with a pattern distribution (FAPD) (3), or lichen planopilaris (LPP) (2), or it may be due to the disease itself (4–6), which we refer to as FFA with crown-area involvement (CA-FFA). Trichoscopic evaluations of patients with FFA and interparietal involvement have identified a diffuse fibrotic pattern in up to 68% of patients (6). However, in the absence of histopathologic confirmation, it remains unclear how many cases truly correspond to CA-FFA or represent an overlap with other alopecia types. To the best of our knowledge, no previous histopathological studies have specifically addressed interparietal involvement in FFA. Clarifying these histopathologic patterns may assist in distinguishing CA-FFA from overlapping forms of scarring alopecia, improving diagnostic accuracy.
2 Materials and methods
We conducted a descriptive and retrospective study enrolling patients with a previous clinical and trichoscopic diagnosis of FFA, in whom interparietal inflammatory findings were observed during follow-up. Participants were evaluated at the Trichology Unit of our center between July and September 2021.
Inflammatory findings were defined as clinical and/or trichoscopic evidence of interparietal involvement and/or symptomatology in that area, including reduced hair density, pruritus, or signs of perifollicular inflammation or scarring, such as perifollicular erythema, perifollicular hyperkeratosis, or loss of follicular ostia.
Two 4-mm interparietal punch biopsies guided by trichoscopy were performed in all patients. The specimens were fixed in formaldehyde, processed in vertical and horizontal sections, and stained with hematoxylin and eosin. The samples were analyzed by the same expert dermatopathologist, following the same protocol. The number of terminal follicles (TFs), miniaturized follicles (MFs), the presence and characteristics of the inflammatory infiltrate, scar tissue, the presence or absence of pigmentary molds, vacuolar degeneration of the follicular epithelium, epithelial or stromal clefting, concentric lamellar fibroplasia, and sebaceous glands were studied in all samples. Informed written consent was obtained from all patients.
This study aimed to characterize the histopathology in the interparietal region of patients with FFA and clinical and/or trichoscopic findings suggestive of inflammation in the interparietal region and/or symptoms in the said location in real clinical practice.
3 Results
A total of 12 Caucasian females with a previous diagnosis of FFA and with a mean age of 64.25 years (range: 49–83) were included. Comorbid conditions included hypothyroidism (n = 5), cutaneous lichen planus (n = 1), breast cancer (n = 1), rosacea (n = 1), psoriasis (n = 1), and genital lichen sclerosus (n = 1).
Following blinded dermatopathologic review, six patients were classified as having findings consistent with CA-FFA (Figure 1A), four patients as having FAPD (Figure 1B), and two patients could not be assigned to either group; however, they both had a cicatricial alopecia with lymphocytic infiltrates. Patients were classified as FAPD if biopsies showed an increased number of vellus hairs, an increase in terminal follicles in catagen and telogen, and a lower terminal-to-vellus ratio (7), and they were classified as CA-FFA if biopsies showed a higher number of follicular scars, more vacuolar degeneration of the follicular epithelium, a greater presence of follicular clefts, and fewer arrector pili muscles (7).
Figure 1. Hematoxylin–eosin (H&E), transverse and horizontal sections. (A) CA-FFA (patient 1): terminal anagen follicles with absence of vellus follicles and absent sebaceous glands, associated with scar tissue and a superficial peri-infundibular lymphocytic infiltrate. (B) FAPD (patient 11): both terminal and vellus/miniaturized follicles, some with superficial lymphocytic infiltrates, together with concentric lamellar fibroplasia.
Detailed histopathologic findings are shown in Table 1. Scar tissue and concentric lamellar fibroplasia were observed consistently across the groups. The inflammatory infiltrate was variable and generally mild to moderate, most often superficial (peri)infundibular, and was absent in some samples. Vacuolar degeneration of the follicular epithelium was uncommon (observed in a single CA-FFA case), and epithelial–stromal clefting occurred infrequently, exclusively within CA-FFA. Sebaceous glands were typically absent or deeply reduced in CA-FFA, reduced but present in FAPD, and focally reduced/normal in indeterminate cases. FAPD biopsies contained more vellus/miniaturized hairs with a shift of terminal follicles toward catagen/telogen, whereas CA-FFA showed greater structural loss.
Table 1. Histopathologic findings in interparietal biopsies.
Patients classified as CA-FFA were older (mean age: 70 vs. 57 years in FAPD) and had longer median disease duration (9 vs. 3 years). Clinical patterns of FFA in the study population were as follows: in the CA-FFA group (n = 6), two patients exhibited Type 1 patterns and four patients exhibited Type 2 patterns; in the FAPD group (n = 4), two patients exhibited Type 1 patterns, one patient exhibited Type 2 patterns, and one patient exhibited Type 3 patterns; and in the indeterminate group (n = 2), one patient exhibited Type 1 patterns and one patient exhibited Type 2 patterns. Overall, diffuse or Type 2 patterns were observed in 66.7% of CA-FFA patients, compared with only 25% of FAPD patients.
Occipital involvement was present in 50% of CA-FFA patients and 25% of FAPD patients; all patients had eyebrow alopecia, and 50% in both groups had body hair involvement. Preauricular wrinkling was the most frequent accompanying facial manifestation across all groups. The majority of participants exhibited a diffuse pattern of clinical interparietal involvement, with the exception of one patient classified as CA-FFA, whose case prompted a reconsideration of the diagnosis, since she exhibited macroscopically visible alopecia plaques, raising the possibility of an overlap between FFA and LPP. Interparietal pruritus was reported by 83% of CA-FFA patients and 50% of FAPD patients. Demographic and clinical characteristics are shown in Table 2. In terms of trichoscopy, the majority of patients in the FAPD group showed evidence of hair miniaturization. The most frequent finding across all groups was perifollicular desquamation.
Table 2. Demographic and clinical characteristics (per patient).
All patients underwent treatment prior to biopsy (Table 3). All but one patient were on systemic therapy, with the vast majority receiving dutasteride (either as monotherapy or in combination). Additionally, seven patients were treated topically in the interparietal region with corticosteroids and/or 5% minoxidil. Treatment distribution was generally balanced across the study groups.
Table 3. Treatments at the time of biopsy.
4 Discussion
After examining interparietal scalp biopsies from patients with a prior diagnosis of FFA who, on follow-up, developed clinical or trichoscopic evidence of inflammatory involvement in that region, we identified two main histopathologic patterns: CA-FFA and FAPD. CA-FFA was characterized by greater structural loss with reduced/absent sebaceous glands and occasional epithelial–stromal clefting. In contrast, FAPD showed more vellus/miniaturized hairs and a shift of terminal follicles toward catagen/telogen, with comparatively milder architectural disruption. Patients with a biopsy suggestive of CA-FFA more often had a diffuse clinical pattern, which implies a worse prognosis, were older, had a longer disease duration, and had approximately twice as much occipital involvement as than those in the FAPD group.
Our results, together with prior observations that FFA causes a greater structural disruption than FAPD (7), raise the question of whether FFA and FAPD represent overlapping entities, as already proposed by other authors, or whether they are part of the same process that has progressed (8, 9). Familial occurrence of both FAPD and FFA has been reported in the literature (10), and the presence in FAPD of features otherwise associated with FFA—such as facial papules (11) and/or extrafacial follicular red dots (12)—prompts us to consider if there is a common pathogenic pathway between both entities.
Furthermore, different authors have proposed considering both entities within a single spectrum. Du et al. described five patients with focal and diffuse fibrosing alopecia with overlapping features and a slowly progressive course (8). However, in contrast to the findings of our study, they noted that younger patients had more evident thinning at the hairline and comparatively mild involvement at the crown, whereas older patients exhibited advanced thinning at both locations. Other authors have proposed that FFA and FAPD may represent the end of a continuum starting from AGA with histologic evidence of follicular microinflammation culminating in clinically noticeable forms of inflammatory cicatricial alopecia (9). They have reported that this hypothesis is reinforced by the overlap of FFA, AGA, and FAPD observed in 11% of their patients, a hypothesis that would also be reinforced by our results.
Moreover, one study found no statistically significant difference in the presence of perifollicular inflammatory infiltrates in affected and unaffected areas of the scalp in patients with FFA (13), while another study showed infundibular and isthmic perifollicular infiltrates in 88% of patients with AGA (14). The reason why these infiltrates do not produce scarring alopecia is still unknown. A plausible explanation is that an as-yet unidentified antigenic stimulus from the damaged hair follicle may trigger a lichenoid tissue reaction in immunologically susceptible individuals (9).
Our study has some limitations. First, the sample size was small, and we did not have a control group. Second, we did not characterize the non-inflammatory interparietal involvement of patients with FFA. Third, our patients underwent treatment prior to the biopsy, which might alter the histopathological results. We may have observed lower-dense inflammatory infiltrates due to the use of corticosteroids and other anti-inflammatory drugs, an increase in anagen follicles due to minoxidil, and a decrease in miniaturized hairs due to the use of 5-alpha-reductase inhibitors. These factors should be considered when interpreting our findings.
5 Conclusion
To the best of our knowledge, we present the first study whose objective is to histopathologically characterize inflammatory interparietal involvement in patients with FFA, having found mainly two patterns. Patients with CA-FFA more frequently presented a diffuse clinical pattern, were older, and had a higher median evolution time than those belonging to the FAPD group.
Further studies in patients with FFA and FAPD are needed to elucidate the presumed role of androgenetic factors in addition to the lymphohistiocytic infiltrate and perifollicular lamellar fibrosis. It remains to be established whether differentiating between FFA and FAPD in the interparietal area may have prognostic and therefore therapeutic implications.
Data availability statement
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
Ethics statement
Ethical approval was not required for the study involving humans in accordance with the local legislation and institutional requirements. Written informed consent to participate in this study was not required from the participants or the participants’ legal guardians/next of kin in accordance with the national legislation and the institutional requirements. Written informed consent was obtained from the individual(s) for the publication of any potentially identifiable images or data included in this article.
Author contributions
MG: Conceptualization, Investigation, Methodology, Writing – original draft, Writing – review & editing, Data curation. AR-V: Conceptualization, Investigation, Methodology, Data curation, Writing – review & editing. DV: Data curation, Writing – review & editing. MV: Conceptualization, Investigation, Methodology, Writing – review & editing. JR: Supervision, Writing – review & editing. AG: Conceptualization, Methodology, Supervision, Writing – review & editing.
Funding
The author(s) declared that financial support was not received for this work and/or its publication.
Conflict of interest
The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Keywords: AGA, androgenetic alopecia, CA-FFA, FAPD, FFA, FFA with crown-area involvement, fibrosing alopecia with a pattern distribution, frontal fibrosing alopecia
Citation: Gonzalez Cañete M, Rodríguez-Villa Lario A, Vega Díez D, Vélez Velazquez MD, Ricart Vaya JM and Gómez Zubiaur A (2026) Histopathological characterization of inflammatory interparietal involvement in frontal fibrosing alopecia. Front. Med. 12:1740090. doi: 10.3389/fmed.2025.1740090
Edited by:
Ruano Juan, University of Cordoba, Córdoba, SpainReviewed by:
Ricardo Ruiz-Villaverde, San Cecilio University Hospital, Granada, SpainDavid Saceda Corralo, Hospital Ramón y Cajal, Madrid, Spain
Copyright © 2026 Gonzalez Cañete, Rodríguez-Villa Lario, Vega Díez, Vélez Velazquez, Ricart Vaya and Gómez Zubiaur. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Marta Gonzalez Cañete, bWFydGFnb25jYW5AbWUuY29t