ORIGINAL RESEARCH article

Front. Mol. Biosci.

Sec. Metabolomics

Volume 12 - 2025 | doi: 10.3389/fmolb.2025.1584132

This article is part of the Research TopicV Latin American Metabolic Profiling Society (LAMPS) Symposium: 2024View all 3 articles

Cold-induced biochemical changes in leaves of two commercial clones of Eucalyptus

Provisionally accepted
Patricia BasilePatricia Basile1*Federico WallaceFederico Wallace2Cristina OlivaroCristina Olivaro2Nicolás De PalmaNicolás De Palma3Omar BorsaniOmar Borsani4Arthur Germano Fett-NetoArthur Germano Fett-Neto3*
  • 1Centro Universitario Regional Noreste, Universidad de la República, Tacuarembo, Uruguay
  • 2Espacio de Ciencia y Tencologia Química, Centro Universitario Regional Noreste, Universidad de la República, Tacuarembo, Uruguay
  • 3Plant Physiology Laboratory, Center for Biotechnology, Federal University of Rio Grande Do Sul (UFRGS), Porto Alegre, Rio de Janeiro, Brazil
  • 4Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay

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Results indicate distinct cold tolerance strategies in E. grandis and E. dunnii. E. dunnii initiated protective mechanism activation after a 2-day exposure period with the accumulation of sugars and phenolic compounds, whereas E. grandis did so after 4 days, accumulating proline and anthocyanins. PLS-DA based on UHPLC-MS fingerprints revealed a clear species-specific effect across the metabolome. This effect was greater than the differences between cold temperatures. Additionally, this methodology allowed the putative identification of 16 phenolic marker compounds with high discriminant potential to differentiate the cold response in these two species.

Keywords: Eucalyptus grandis 1, Eucalyptus dunnii 2, cold stress 3, biochemical responses 4, metabolomic 5

Received: 26 Feb 2025; Accepted: 21 May 2025.

Copyright: © 2025 Basile, Wallace, Olivaro, De Palma, Borsani and Germano Fett-Neto. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Patricia Basile, Centro Universitario Regional Noreste, Universidad de la República, Tacuarembo, Uruguay
Arthur Germano Fett-Neto, Plant Physiology Laboratory, Center for Biotechnology, Federal University of Rio Grande Do Sul (UFRGS), Porto Alegre, Rio de Janeiro, Brazil

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Supplementary Material