ORIGINAL RESEARCH article
Front. Mol. Biosci.
Sec. Metabolomics
Volume 12 - 2025 | doi: 10.3389/fmolb.2025.1644637
Lipidomic Profiling Reveals Systemic Serum Lipid Remodeling Induced by Dexamethasone in Mice
Provisionally accepted
- 1Jiangning Center for Disease Control and Prevention, Nanjing, China
- 2Nanjing University of Chinese Medicine, Nanjing, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
Dexamethasone is a commonly used glucocorticoid medication in pediatrics. However, dexamethasone is associated with some side effects, such as dyslipidemia. Herein, we utilized Exactive Orbitrap-MS technology to assess the effects of dexamethasone intervention on serum lipids in mice. Unbiased Principal Component Analysis (PCA) revealed that dexamethasone intervention significantly induced changes in serum lipids in mice, and after a 7-day washout period (equivalent to 28 drug half-lives), changes of lipids in the serum were still existed compared with those in the control groups. After 4 days of dexamethasone injection, significant changes were observed, including 16 increased lipids, and 1 decreased lipid in the serum compared with those in the control groups. After a 7-day washout period, some of lipids in the serum were still changed, including 5 increased lipids, such as Acylcarnitines (CAR), ceramides (Cer), diacylglycerophosphates (DG), lysophosphatidylglycerol (LPG) and phosphatidylglycerol (PG), 1 decreased lipid, hexosylceramides (HexCer), indicating dexamethasone may result in long-term changes of lipids in the serum. In conclusion, utilizing a lipidomics method, we provide the first complete proof that dexamethasone intervention generates extensive modification of the serum lipidome.
Keywords: Blood lipids, Dexamethasone, Exactive Orbitrap-MS technology, lipidomic, lipid remodeling
Received: 13 Jun 2025; Accepted: 28 Aug 2025.
Copyright: © 2025 Zhang, Wang and Ji. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Jianjian Ji, Nanjing University of Chinese Medicine, Nanjing, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.