Corrigendum: A Novel RNA Editing Sensor Tool and a Specific Agonist Determine Neuronal Protein Expression of RNA-Edited Glycine Receptors and Identify a Genomic APOBEC1 Dimorphism as a New Genetic Risk Factor of Epilepsy

In the original article, there was an error. The incorrect oligonucleotide sequences were provided.

A correction has been made to the Methods, subsection PCR-RFLP Analysis of Human TLE Samples:

“Resected hippocampal tissue of human iTLE patients (Eichler et al., 2008) was analyzed with regard to APOBEC1 gene dimorphism coding for 80M or 80I Apobec-1 protein variants. For this purpose, we developed a new PCR-based RFLP approach. Total RNA was isolated and reverse transcribed into cDNA as described earlier (Raltschev et al., 2016). Pre-amplification of Apobec-1 was performed using oligonucleotides 5′-CTTCAACCGGTGACCCCACTC-3′ and 5′-TGCGTACAACATCATCCACAGAGG-3′. Then, 3.5 μl of the pre-PCR were investigated in another PCR using oligonucleotides 5′-GAGTTTGACGTCTTCTATGACCC-3′ and 5′-GTTGACAAAATTCCTCCAGCAG-3′ to amplify a region spanning the 80M/I-coding position. This nested PCR amplification step yielded sufficient amount of DNA that was purified with Monarch® DNA Gel Extraction Kit (catalog no. L1020L, New England Biolabs GmbH) and digested using NlaIII restriction enzyme. NlaIII cuts at the 80M-coding position (CATG), and restriction fragments were separated using electrophoresis with 5% agarose gels to identify the genotype of the iTLE patients. For control purpose, Apobec-1 80I- or 80M-coding vectors for transfection were processed in parallel. Ethidium bromide was used to stain DNA bands.”

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Summary

Keywords

glycine receptors, epilepsy, temporal lobe, RNA editing, hippocampus, ligands

Citation

Kankowski S, Förstera B, Winkelmann A, Knauff P, Wanker EE, You XA, Semtner M, Hetsch F and Meier JC (2019) Corrigendum: A Novel RNA Editing Sensor Tool and a Specific Agonist Determine Neuronal Protein Expression of RNA-Edited Glycine Receptors and Identify a Genomic APOBEC1 Dimorphism as a New Genetic Risk Factor of Epilepsy. Front. Mol. Neurosci. 12:103. doi: 10.3389/fnmol.2019.00103

Received

26 March 2019

Accepted

04 April 2019

Published

24 April 2019

Volume

12 - 2019

Edited and reviewed by

Carmen Villmann, University of Wuerzburg, Germany

Updates

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Copyright

© 2019 Kankowski, Förstera, Winkelmann, Knauff, Wanker, You, Semtner, Hetsch and Meier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Florian Hetsch f.hetsch@tu-braunschweig.deJochen C. Meier jochen.meier@tu-braunschweig.de

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