Abstract
Visual processing starts at the retina of the eye, and signals are then transferred primarily to the visual cortex and the tectum. In the retina, multiple neural networks encode different aspects of visual input, such as color and motion. Subsequently, multiple neural streams in parallel convey unique aspects of visual information to cortical and subcortical regions. Bipolar cells, which are the second-order neurons of the retina, separate visual signals evoked by light and dark contrasts and encode them to ON and OFF pathways, respectively. The interplay between ON and OFF neural signals is the foundation for visual processing for object contrast which underlies higher order stimulus processing. ON and OFF pathways have been classically thought to signal in a mirror-symmetric manner. However, while these two pathways contribute synergistically to visual perception in some instances, they have pronounced asymmetries suggesting independent operation in other cases. In this review, we summarize the role of the ON–OFF dichotomy in visual signaling, aiming to contribute to the understanding of visual recognition.
1 Introduction
Foundational processing of the visual environment occurs in the retina of the eyes, the lateral geniculate nucleus (LGN) in the thalamus, the primary visual cortex (V1), where visual recognition occurs, and the superior colliculus (SC) in the tectum, a center for eye movement (Figure 1). Until several decades ago, the function of the retina was thought to involve capturing visual signals, much like a camera film, and then sending them to the brain. Recent evidence has suggested that the retina contains numerous intricate neural networks that analyze the captured visual signals and actively starting visual signal processing. Understanding how the entire visual system conducts visual signal processing is the ultimate goal of vision research. However, the critical centers of the visual system are located far from each other, rendering it difficult to undertake a systematic investigation. This review focuses on a set of classical visual pathways, the ON and OFF pathways, and discusses how they are generated, conveyed, and utilized throughout the visual system.
Figure 1
Visual signal recognition is achieved by parallel processing, that is, multiple features of visual signals are encoded into separate neural streams and analyzed in parallel (). Parallel processing is initiated by separation of visual signals in retinal neurons, including photoreceptors, bipolar cells, and ganglion cells, and are coded to multiple types of retinal ganglion cells. Each visual pathway is then sent to unique sets of destinations in the brain. In the primate retina, two main pathways have been identified as being involved in parallel processing, namely, the magnocellular pathway, which encodes motion and changes in the visual scene, and the parvocellular pathway, which encodes the shape and color of visual information (, ).
Parallel processing is initiated at the photoreceptors, the first-order neurons in the retina, where color information is separated by cone photoreceptors with different wavelength sensitivities (–). Visual signals are then transmitted from photoreceptors to the second-order neurons, bipolar cells, where signals are further subdivided. Approximately 15 subtypes of bipolar cells have been identified in the retinas of vertebrates, including primates, rodents, fish, birds, and salamanders (Figure 2) (–). Each type of bipolar cell is thought to extract unique features of visual scenes and initiate distinct parallel signaling pathways. Two well-studied bipolar cell pathways are chromatic signaling pathways (–) and ON/OFF-signaling pathways (, , ).
Figure 2
The ON–OFF separation occurs at the dendrites of bipolar cells (discussed in more detail below). The retina senses the contrast of an object, either bright or dark, against the background luminance. Half of the bipolar cells depolarize in response to a visual signal brighter than the background, whereas the other half hyperpolarizes in response to the bright object, but depolarizes when the illumination ends (Figure 3). Cells that respond to the onset of light (or offset of dark) are ON bipolar cells (Figure 3C) while cells responding to light offset (or onset of dark) are OFF bipolar cells (Figure 3B). Signals in ON and OFF bipolar cells are transferred to ON- and OFF-responding ganglion cells, respectively. A subset of ganglion cells collects both ON and OFF signals, which are described as ON-OFF ganglion cells. Then, ON, OFF, and ON-OFF ganglion cells send their signals to a unique set of targeted neurons in the LGN and other parts of the brain to achieve visual perception.
Figure 3
Separated ON and OFF visual signals in the retina are conveyed through independent parallel processing neural streams to the brain where ON and OFF signals start to combine. Because ON and OFF signaling pathways convey the light and dark signaling of the same object, it has been classically thought to signal in a mirror-symmetric manner. However, asymmetric ON and OFF signaling has been reported in the retina and V1. Furthermore, ON and OFF signal coordination outlines the features of the object for some object recognition (e.g. orientation tuning and direction selectivity), whereas ON and OFF signals separately operate to achieve the object recognition for other object recognitions (e.g. optokinetic reflex (OKR) and looming stimulus-evoked fear responses). In this review, we focus on ON–OFF signaling to explore how the visual system executes the visual recognition process.
2 On and off signaling in the visual system
ON and OFF signaling pathways are separated at the retinal photoreceptor-bipolar cell synapse. The separated signals are transmitted to amacrine and ganglion cells in the retina, which are transferred to LGN neurons. The separated ON and OFF signaling streams are subsequently integrated into the visual cortex and superior colliculus (SC).
2.1 Retina
Both rod and cone photoreceptors hyperpolarize to light and depolarize under dark conditions (Figure 3A). In the dark, photoreceptors are depolarized and continuously release the neurotransmitter glutamate, thereby providing signals to subsequent neurons, the bipolar cells. OFF bipolar cells possess ionotropic glutamate receptors and depolarize in the dark in response to glutamate released by the photoreceptors (–). In contrast, ON bipolar cells express the metabotropic glutamate receptors, mGluR6, on their dendrites. mGluR6 links to the G protein, Go, and consequently, the downstream second-messenger system, which leads to the cell hyperpolarization following glutamate binding (, ). Therefore, ON bipolar cells hyperpolarize in the dark, which is the opposite sign of photoreceptors and OFF bipolar cells.
When the retina is illuminated, photoreceptors hyperpolarize and reduce their glutamate release.ON bipolar cells are free from the suppressive effect of glutamate binding to mGluR6, resulting in activation of the mGluR6-linked cation channel, TRP M1, to depolarize the bipolar cell (Figure 3C) (–). In contrast, reduced glutamate release from photoreceptors leads to hyperpolarization of OFF bipolar cells (Figure 3B). In brief, ON bipolar cells hyperpolarize in the dark, which is the opposite of that seen for photoreceptors and OFF bipolar cells. In response to light, ON bipolar cells depolarize, and OFF bipolar cells hyperpolarize. Accordingly, bipolar cells are the site of ON and OFF signaling separation.
The ON and OFF dichotomy is received by downstream amacrine and ganglion cells. Signal transfer occurs in the inner plexiform layer (IPL), where ON and OFF cells ramify in separate layers (Figure 2). The outer half of the IPL is the OFF-signaling layer and is where OFF bipolar, amacrine, and ganglion cells make synaptic connections, while the inner half of the IPL is where ON bipolar, amacrine, and ganglion cells extend their processes for the synaptic transmission of ON signaling. Dozens of ganglion cell types have been recently identified in the retinas of mice (, ) and primates (, ), with most being categorized as either ON, OFF, or ON-OFF ganglion cells based on their ramification patterns in the IPL. Retinal ganglion cells extend their axons to the brain forming the optic nerve, through which the signals are transmitted.
ON and OFF bipolar cells innervate ganglion cells that include dozens of types. Many types of ganglion cells possess ON and OFF counterparts, such as ON brisk-transient and OFF brisk-transient ganglion cells. Ravi et al. () recorded spikes from ON and OFF pairs of brisk-sustained, brisk-transient, and small transient ganglion cells and found asymmetries between ON and OFF signals. ON brisk-sustained cells possess a larger receptive field and respond briefer than their OFF counterparts. In contrast, ON brisk-transient ganglion cells possess a smaller receptive field and respond more sustained than their OFF counterparts, and ON and OFF small-transient ganglion cells have similar spatial and temporal profiles. Asymmetries in temporal, spatial, and linearity characteristics between ON and OFF ganglion cells of the same types have been reported in the mouse retina (, ) and the macaque retina (, ).
The ON and OFF asymmetry in temporal aspects probably does not arise due to the different glutamate receptors present in OFF and ON bipolar cells because in the mammalian retina, there are no significant temporal differences between ON and OFF bipolar/ganglion cells (, , , ). Instead, the ON and OFF asymmetry is in a ganglion cell type-dependent manner, suggesting that the combination of bipolar cell inputs and amacrine cell inputs to a type of ganglion cells shapes the asymmetry.
2.2 Lateral geniculate nucleus to the primary visual cortex
2.2.1 Lateral geniculate nucleus
Many types of ganglion cells project to multiple locations in the central nervous system. The lateral geniculate nucleus (LGN) is a major target for ganglion cells (Figure 1), which is divided into dorsal and ventral parts. Image-forming ganglion cells target the dorsal LGN (dLGN), relaying visual signals from the retina to the visual cortex (–). In contrast, non-image-forming ganglion cells, including the intrinsic photosensitive retinal ganglion cells (ipRGCs), project to the ventral LGN (, ). These observations indicate that visual stimulation-evoked responses take place in the dLGN.
In the dLGN, parvocellular and magnocellular ganglion cells from ipsilateral and contralateral eyes project into multiple distinct layers. The ON–OFF dichotomy is preserved in each region of the dLGN (), suggesting that the mixing of ON/OFF signals does not occur in the thalamic structure. Koniocellular LGN neurons exhibit transient combined ON–OFF responses, which may originate from broad-thorny ON–OFF retinal ganglion cells () and not from separate ON and OFF ganglion cells. This case also suggests that ON and OFF signaling is relayed as transferred from the retina.
In the mouse and rat dLGN, color-responsive cells exhibit ON and OFF dichotomy (, ). Chromatic cells show color opponency with opposite ON and OFF responses when activated by green or UV light. In the mouse retina, cone photoreceptors contain two types of opsins, green and UV (), which are transferred to green-OFF (Type 1) and UV-ON (Type 9) chromatic bipolar cells (, ). Concomitantly, some types of ON ganglion cells exhibit color opponency in a region of the retina, with green light evoking OFF responses and UV light triggering ON responses, consistent with the signs of chromatic bipolar cells (). Most color-opponent cells in the dLGN show the same color response signs, also suggesting that dLGN neurons receive ON and OFF signals from retinal ganglion cells and relay the signals to the subsequent neurons without mingling the signals.
2.2.2 Primary visual cortex (V1)
The ON and OFF pathways are still separated in the dLGN, which are conveyed and integrated into the primary visual cortex (V1). The V1 is composed of six layers of cytoarchitecture. Thalamocortical fibers entering area V1 from the dLGN project mainly to Layer 4 but also partly to Layer 6. Layer 4 fibers primarily project to Layer 2/3 in the visual cortices of cats, monkeys, and tree shrews (–). In addition to the thalamocortical inputs, neurons make feedforward and feedback connections among layers. Furthermore, there is also considerable feedback input to the LGN and projection to the SC from Layer 5 (Figure 4A).
Figure 4
The V1 contains both simple cells and complex cells. Simple cells are mainly found in Layer 4 and receive input through the thalamocortical fibers (). The receptive fields of many simple cells have adjoining, elongated ON and OFF subfields (Figure 4B) (). This elongated shape differs from the shape of receptive fields of the dLGN and retinal ganglion cells, which show a concentric ON or OFF center with an antagonistic surround. The different shapes are attributable to the convergence of thalamocortical inputs; multiple thalamic neurons in line innervate one simple cell in V1 (). Complex cells reside outside of Layer 4, which are innervated by simple cells (, ), and have greater ON and OFF signals overlap (Figure 4C) ().
ON and OFF signals in V1 are transferred from the dLGN in parallel. Interestingly, these two inputs appeared to be asymmetric in terms of spatial and temporal aspects. In the temporal aspect, the OFF pathway is faster than the ON pathway in cat V1 (, ). In line with this, a large, long-lasting stimulus evokes stronger ON than OFF responses, whereas the opposite is seen when a small, fast stimulus is applied (). The origin of the ON/OFF temporal asymmetry might be originated in the retina, but it has not been fully understood. The ON/OFF temporal difference indicates that dark signals are recognized faster than the light signals.
ON/OFF asymmetry is also observed in the mouse visual cortex. OFF responses dominate in the central visual field where binocular innervation occurs. In contrast, ON and OFF responses are more balanced in the periphery ().
In the spatial aspect, ON/OFF asymmetry is also detected in the size of receptive field and neuronal linearity/nonlinearity in the LGN and V1 of cats () and humans (). OFF-center cells dominate the areas in V1 of cats () and macaque V1 (, ), which facilitates the contrast discrimination in the natural scene (). These features would cause dark stimuli to drive cortical neurons more strongly than light stimuli at low spatial frequencies.
2.3 Superior colliculus
The SC is a midbrain structure and the integrating center for eye movements (Figures 1, 5). Although the SC communicates with the motor, auditory, and visual systems, the superficial layers of the SC are purely visual (Figure 5B). Retinal ganglion cells directly project their axons to this region, which preserves retinotopic organization (, ). Approximately five to six ON and OFF ganglion cells provide synaptic inputs to one SC cell in mice (, ).
Figure 5
Most SC cells are ON and OFF responding (, ). Receptive field mapping showed that most SC neurons have spatially overlapping ON and OFF subfields (Figure 5C) (), indicating that the corresponding ganglion cells are neighboring ON and OFF ganglion cells. Interestingly, one study indicated that when the visual cortex is removed, the ON and OFF overlap ratio is decreased (), suggesting that the corticocollicular inputs, which connect from V1 cortex to the SC, adjust the receptive fields of SC cells. The corticocollicular pathway originates in Layer 5 of V1, where most cells are complex cells with overlapping ON and OFF receptive fields (Figure 4). The high ON and OFF overlapping in V1 Layer 5 presumably increases the ON and OFF overlap ratio in SC cells. This observation also suggests that separate ON and OFF ganglion cells, and possibly also some ON-OFF ganglion cells, provide synaptic inputs to single SC cells.
3 Unified on and off functions
ON and OFF signals are separated in the retina and integrated into V1 cortex and SC. In this section, we address how the ON and OFF signals contribute to specific visual functions, focusing on two visual functions that result from the synergistical contributions of ON and OFF signals. In the following section, we introduce two visual functions that are achieved by signaling independently through either ON or OFF pathway.
3.1 Orientation tuning
Several decades ago, Hubel and Wiesel identified the orientation column in the V1 of cats (, ). An orientation column is a unit of functional architecture of V1 in which neurons in a columnar region (a small area that encompasses Layer 1 through Layer 6) strongly respond to a bar of a particular orientation (Figure 6A). Neurons in neighboring columns show slightly different angle preferences, while neurons in a larger area exhibit preferences to all angles (Figure 6B). The orientation preference is determined by the shape of the receptive field of the simple cell in the column, which is elongated and responds to a bar that fits the angle (Figure 4B). In addition to cats, functional columnar structure and orientation columns are also present in the V1 of primates (, ), tree shrews (, ), and ferrets ().
Figure 6
Recent evidence has shown that the receptive fields of V1 single cells contain ON and OFF subfields, and the arrangement of these subfields is a crucial determinant of the preferences of orientation columns. In the V1 of the tree shrews, the receptive field of a single cell often contains a central OFF subfield flanked by two ON subfields, and the arrangement of the two ON subfields predicts the orientation preference of the column (Figure 6B) (, ). A similar arrangement is observed in simple cells of the cat V1, which exhibit elongated ON and OFF subfields aligned according to their orientation preferences (, ). These observations suggest that ON and OFF cells coordinate to generate the orientation tuning.
In contrast to the cortices of primates and other mammals, the orientation column is not well developed in the V1 of some species, including rabbits and mice (, ). Mouse V1 simple cells have ON and OFF subfields within the receptive field; however, there is a lack of identifiable orientation columnar structure (). Interestingly, in both mice and rabbits, the orientation-selective ganglion cells in the retina appear to be responsible for generating orientation tuning (–). Their signs are almost exclusively ON-responding; however, the OFF pathway might generate their antagonistic receptive fields. The orientation tuning mechanism seems to differ between rodents and primates/cats, but the arrangement of both ON and OFF pathways is crucial in all species examined to date.
3.2 Direction selectivity
Direction selectivity is a form of motion detection that is first coded in direction-selective ganglion cells (DSGCs) in the retinas of many vertebrates, including mice, fish, and rabbits (, , –). Starburst amacrine cells (SACs), which release GABA onto DSGCs asymmetrically, shape the direction-selectivity of DSGCs (98, 99). DSGCs generate spikes in response to the movement of an object in a specific direction. Each DSGC senses one of the cardinal directions, namely, dorsal, ventral, nasal, or temporal (Figure 7). DSGCs display ON, OFF, or ON–OFF signs. However, the existence of DSGCs in the primate retina remains controversial, and there is both evidence for (100, 101) and against (102, 103) this possibility.
Figure 7
The SC is a critical target for retinal direction selectivity, which has been reported in SC cells of many species, including fish, mice, and monkeys (, , 104–106). The receptive field of SC cells contains several ganglion cells with ON, OFF, and ON-OFF signs. Most SC cells respond to both the onset and offset of visual stimuli: ON-OFF responding cells (, ).
The ON and OFF signs of retinal DSGCs are species-dependent. ON, OFF, and ON-OFF DSGCs are found in the mouse retina (); ON and ON-OFF DSGCs but not OFF DSGCs are found in the rabbit retina (107, 108); and ON and OFF DSGCs but not ON-OFF DSGCs are present in the fish retina (109, 110). However, single SC cells receive both ON and OFF signals and show ON-OFF responses (110), suggesting that the motion signal, and not the polarity of contrast (darkness or brightness) is essential information for SC cells.
SC cells and retinal ganglion cells also display differences in directional preference. In the fish retina, DSGCs show three directional preferences, namely, dorso-ventral, ventro-dorsal, and caudo-rostral. However, in the fish tectum of the midbrain, SC neurons also show a rostro-caudal directional preference (106). Similarly, in the mouse retina, DSGCs exhibit only one of the four cardinal directional preferences (dorsal, ventral, nasal, or temporal) (Figure 7), whereas SC neurons exhibit a full range of preferred directions (104). Notably, SC neurons receive feedback and feedforward inputs from multiple regions of the central nervous system, and these may be the source of the additional directional preference.
In summary, SC neurons preserve the orientation of the retinotopic map, hold both ON and OFF signals from the retina in their receptive fields, and exhibit unique preferred directions. These properties indicate that SC neurons code the spatial extent and direction of a moving object to initiate smooth pursuit or foveation. For direction selectivity in the SC, retinal ON and OFF cells are the contrast detectors, perceiving the direction of both bright and dark moving objects.
4 Independent on and off functions
4.1 Optokinetic reflex
The optokinetic reflex (OKR) is a visual reflex that helps most vertebrates stabilize retinal images in relation to movements in the visual world. OKR testing comprises slow eye-tracking of the moving vertical stripes in a rotating grating followed by a rapid saccade in the opposite direction, which returns the eye to its primary position (Figure 8A) (111). The OKR test is utilized to measure visual acuity and contrast sensitivity in laboratory animals (112), and different models of optomotor devices have been developed to obtain reliable optokinetic responses (113, 114).
Figure 8
Visual kinetic information is sensed by retinal motion detectors; SACs and DSGCs. Detected signals are transmitted through the optic nerve and the optic tract to the visual cortex and SC. The accessory optic system (AOS) contributes to the pathway and receives afferent connections from the nuclei of the optic tract (NOT), the dorsal terminal nucleus (dTN), and the LGN. Efferent connections of the AOS target the brainstem and cerebellar nuclei, which activate the motor neurons of the extraocular muscles (115–117). Using functional ultrasound imaging of the whole brain, Mace et al. (118) identified 87 brain regions that exhibit changes in activity in relation to the OKR.
Some evidence have shown that OKR depends exclusively on the ON pathway and not the OFF pathway. A zebrafish mutant, no optokinetic response c (nrc), lacks an OKR (119), of which processes invaginated into the photoreceptor terminals and the ribbons in most photoreceptor pedicles appeared to be free-floating in the cytoplasm, indicating that the synaptic connections between photoreceptors and ON bipolar cells were disrupted. Additionally, Emran et al. (120) reported that most of the cells in nrc mutants display an OFF function and no pure ON ganglion cells could be detected based on electroretinogram (ERG) and single-unit recordings, and pharmacological blockade of ON pathway in wild-type zebrafish mimicked the nrc mutant phenotype.
A study was done by Aung et al. (121) on nob and Vsx1-/- mice which have dysfunctional ON, and partial dysfunction in OFF pathways, respectively. They used spatial frequency and contrast sensitivity thresholds to assess the OKR and reported that both mutants exhibited reduction in OKR. However, the findings noticed in Vsx1-/- mice were significantly lower than those observed in nob mice. This is another evidence that ON pathway is crucial for the OKR, while OFF pathway contributes minimally to the reflex.
Joly et al. (122) used ischemia/reperfusion (I/R) to identify the direction-selective circuits linked to the OKR in mice. Ligation of the central ophthalmic artery and vein for 60 minutes caused irreversible loss of the OKR. Histological analysis revealed that ON SACs and ON DSGCs were markedly affected by I/R, whereas OFF SACs were only minimally impacted. Specifically, notable dendritic loss was observed in the DSGCs at DSGC-ON SAC synaptic connections. The results of this study also showed that the ON pathway is critical for the OKR.
Interestingly, Drosophila model exhibited that both ON and OFF pathways are necessary to evoke OKR, while photoreceptor input to either ON or OFF pathway alone is sufficient to sense the moving grating. This paradox is attributable to the separation of photoreceptors output into ON and OFF channels in the lamina and the electrical coupling happening between both pathways which enables cross activity, so a single pathway is activated when only its counterpart is receiving the input (123).
4.2 Looming-evoked fear response
The neural pathways responsible for fear responses have recently been explored. When a dark object suddenly approaches, animals react to the object by quickly escaping from it or freezing. The looming stimulus of a rapidly-expanding black disk displayed on a ceiling monitor mimics the approaching dark object, and induces a freezing or rapid flight response in mice (Figure 8B) (124, 125). Therefore, the looming stimulus can be used as a vision test if a mouse sees a black object and responds with escaping or freezing.
Because an expanding dark spot with moving edges evokes fear responses, OFF direction-selective cells in the retina are expected to contribute to looming-evoked behavior (124). Two types of OFF cell pathways have been shown to convey looming-evoked fear responses in mice: the PV-5 OFF ganglion cells (126), and a neural pathway of VG3 amacrine cells - W3 & OFF t-α ganglion cells (127, 128). The VG3 (vesicular glutamate transporter 3) cells are one of more than 60 types of amacrine cells that contain glutamate as their neurotransmitter. W3 and OFF t-α cells are types of ganglion cells, which receive synaptic inputs from the VG3 amacrine cells.
VG3 cells depolarize to looming stimuli and hyperpolarize to receding stimuli, leading to the unique innervation of two types of ganglion cells, W3 and OFFα. VG3 cells respond at the onset of motion by transient excitation followed by sustained inhibition, with amplitudes proportional to the speed of the stimulus. W3 cells receive a similar excitation and inhibition pattern and are believed to signal the onset of the looming stimulus. Meanwhile, OFFα ganglion cells respond during looming, and their excitation corresponds to the speed of the stimulus. Therefore, slight delays between excitation and inhibition of both W3 and OFFα ganglion cells inform the brain regarding the onset and speed of stimulus, respectively. The ablation of these neurons eliminates the defensive responses to looming stimuli (127, 128), indicating that the pathway of VG3-W3/OFFα ganglion cells is critical for looming stimulus-evoked fear responses.
Looming-evoked fear responses are induced in both vertebrates and invertebrates. In the fly eye, a looming detector has been identified, named type II (LPLC2) neuron in the lobula plate/lobula columnar (129), which is analogous to the SC of vertebrates (130). These cells respond strongly to a looming stimulus, but weakly to a moving stimulus in a lateral direction. Similarly, in the locust eye, the lobula giant movement detector (LGMD) and its downstream neurons are designated looming detectors (131, 132). These cells respond only to dark, indicating that the looming responses are mediated only by OFF-responding neurons.
5 On-off polarity switch
Recent evidence suggests that the retinal neural hardware is more flexible than was previously thought (133). Rivlin-Etzion and her group (134) reported that in ON SACs, a certain light stimulus switched the status of light-evoked responses from depolarized to hyperpolarized. Because SACs are a crucial component of direction selectivity in the retina, the polarity switch in the SACs changed the direction selectivity in DSGCs (135, 136). Additionally, Tikidji-Hamburyan et al. (137) and Pearson and Kerschensteiner (138) found that ON and OFF spikes in ON-OFF ganglion cells were differently generated over a broad range of ambient light levels from scotopic to photopic conditions, resulting in the switching of the ON-dominance and OFF-dominance at different ambient light conditions. Furthermore, Sagdullaev and Mccall (139) and Farrow et al. (140) reported that a bright full-field light stimulus altered ON and OFF polarity in some ganglion cells, a result that was attributable to wide-field amacrine cell inputs that convey signals to distant regions within the retina. Pang et al. (141) and Hoshi et al. (142) suggested that glutamatergic AMPA receptors in ON bipolar cells may also induce cross excitation. Together, these reports provide evidence of a polarity switch between ON and OFF signs in the retina.
The ON and OFF sign switch might be a crucial mechanism for adapting to the saccadic image shift (143, 144). Additionally, the switch might play a role in coding a complicated natural scene (145, 146). How the ON and OFF polarity switch in the retina affects the central projection has not been explored, and future studies should focus on elucidating the ON and OFF pathways and signals in a broad range of visual environments.
6 Clinical conditions
Vision disorders in humans regarding the ON and OFF pathways have been reported. Sieving et al. (147) reported that a human patient with unilateral cone dystrophy exhibited an abnormal electroretinogram (ERG) with a sustained positive plateau instead of a standard transient b-wave. The patient’s ERG is similar to a photopic macaque ERG when ionotropic glutamate receptors were blocked pharmacologically, indicating that the OFF pathway is disrupted in this patient. The major complaint of cone dystrophy, including this patient, is decreased color vision (147, 148), which might be originated from the cone dysfunction and the reduced OFF pathway signaling might not be related to the symptom. This is a rare disorder, and physio-pathological mechanisms have not been fully understood.
Vision disorder with a loss of ON pathway has been reported as the congenital stationary night-blindness (CSNB) (149). ERG for these patients shows a reduced or abolished scotopic b-wave, which indicates that the signal transmission from rod photoreceptors to rod bipolar cells, scotopic ON signaling, is disrupted. These patients complain of night blindness, but many of them show normal vision in the daytime. There is a broad spectrum for this disorder; the complete form CSNB patients show the eliminated scotopic b-wave but normal cone photoreceptor functions, whereas the incomplete form CSNB patients exhibit reduced scotopic b-wave and reduced cone functions. Pathological mechanisms include the dysfunction of mGluR6-TrpM1 complex and Ca++ channel disorders (150, 151).
7 Conclusion
Although the visual system is complicated and only poorly elucidated, it is increasingly accepted that parallel processing represents a crucial mechanism for visual recognition. The ON/OFF dichotomy is generated in the retina through the sensing of the contrast of an object against the background luminance. ON and OFF signaling synergistically or independently conveys the features of the object. Specifically, orientation tuning and direction selectivity are achieved by the interaction between ON and OFF pathways, while the OKR and looming-evoked fear responses are mediated by either ON or OFF pathways independently. This suggests that visual recognition through the cerebral cortex utilizes both pathways, but reflexes use only one of them to promptly respond to a visual scene. Studying how the signals are subsequently transferred to the visual system is crucial for understanding visual signal processing.
Funding
The present study was supported by the National Institutes of Health (NIH) project grants (R01 EY028915; R01 EY032917), and by Research to Prevent Blindness (RPB).
Acknowledgments
We thank Mr. Jeremy Bohl for critically reading the manuscript and Dr. Reece Mazade for valuable and insightful suggestions.
Publisher’s note
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Statements
Author contributions
TI designed the article, and TI and SH wrote the manuscript. All authors contributed to the article and approved the submitted version.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
1
WassleH. Parallel processing in the mammalian retina. Nat Rev Neurosci (2004) 5:747–57. doi: 10.1038/nrn1497
2
LivingstoneMSHubelDH. Psychophysical evidence for separate channels for the perception of form, color, movement, and depth. J Neurosci (1987) 7:3416–68. doi: 10.1523/JNEUROSCI.07-11-03416.1987
3
LivingstoneMHubelD. Segregation of form, color, movement, and depth: Anatomy, physiology, and perception. Science (1988) 240:740–9. doi: 10.1126/science.3283936
4
BaylorDA. Photoreceptor signals and vision. proctor lecture. Invest Ophthalmol Vis Sci (1987) 28:34–49.
5
SchmidtBPBoehmAETutenWSRoordaA. Spatial summation of individual cones in human color vision. PloS One (2019) 14:E0211397. doi: 10.1371/journal.pone.0211397
6
HoferHCarrollJNeitzJNeitzMWilliamsDR. Organization of the human trichromatic cone mosaic. J Neurosci (2005) 25:9669–79. doi: 10.1523/JNEUROSCI.2414-05.2005
7
SchivizANRufTKuebber-HeissASchubertCAhneltPK. Retinal cone topography of artiodactyl mammals: Influence of body height and habitat. J Comp Neurol (2008) 507:1336–50. doi: 10.1002/cne.21626
8
AhneltPKSchubertCKubber-HeissASchivizAAngerE. Independent variation of retinal s and m cone photoreceptor topographies: A survey of four families of mammals. Vis Neurosci (2006) 23:429–35. doi: 10.1017/S095252380623342X
9
HaverkampSWassleHDuebelJKunerTAugustineGJFengGet al. The primordial, blue-cone color system of the mouse retina. J Neurosci (2005) 25:5438–45. doi: 10.1523/JNEUROSCI.1117-05.2005
10
PattersonSSNeitzMNeitzJ. S-cone circuits in the primate retina for non-Image-Forming vision. Semin Cell Dev Biol (2022) 126:66–70. doi: 10.1016/j.semcdb.2021.05.004
11
TsukamotoYOmiN. On bipolar cells in macaque retina: Type-specific synaptic connectivity with special reference to off counterparts. Front Neuroanat (2016) 10:104. doi: 10.3389/fnana.2016.00104
12
TsukamotoYOmiN. Some off bipolar cell types make contact with both rods and cones in macaque and mouse retinas. Front Neuroanat (2014) 8:105. doi: 10.3389/fnana.2014.00105
13
WuSMGaoFMapleBR. Functional architecture of synapses in the inner retina: Segregation of visual signals by stratification of bipolar cell axon terminals. J Neurosci (2000) 20:4462–70. doi: 10.1523/JNEUROSCI.20-12-04462.2000
14
EulerTWassleH. Immunocytochemical identification of cone bipolar cells in the rat retina. J Comp Neurol (1995) 361:461–78. doi: 10.1002/cne.903610310
15
BoycottBBWassleH. Morphological classification of bipolar cells of the primate retina. Eur J Neurosci (1991) 3:1069–88. doi: 10.1111/j.1460-9568.1991.tb00043.x
16
HaverkampSHaeseleerFHendricksonA. A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina. Vis Neurosci (2003) 20:589–600. doi: 10.1017/S0952523803206015
17
WassleHPullerCMullerFHaverkampS. Cone contacts, mosaics, and territories of bipolar cells in the mouse retina. J Neurosci (2009) 29:106–17. doi: 10.1523/JNEUROSCI.4442-08.2009
18
ShekharKLapanSWWhitneyIETranNMMacoskoEZKowalczykMet al. Comprehensive classification of retinal bipolar neurons by single-cell transcriptomics. Cell (2016) 166:1308–1323 E30. doi: 10.1016/j.cell.2016.07.054
19
HelmstaedterMBriggmanKLTuragaSCJainVSeungHSDenkW. Connectomic reconstruction of the inner plexiform layer in the mouse retina. Nature (2013) 500:168–74. doi: 10.1038/nature12346
20
BorghuisBGMarvinJSLoogerLLDembJB. Two-photon imaging of nonlinear glutamate release dynamics At bipolar cell synapses in the mouse retina. J Neurosci (2013) 33:10972–85. doi: 10.1523/JNEUROSCI.1241-13.2013
21
YamagataMYanWSanesJR. A cell atlas of the chick retina based on single-cell transcriptomics. Elife (2021) 10:1–39. doi: 10.7554/eLife.63907.sa2
22
CrookJDManookinMBPackerOSDaceyDM. Horizontal cell feedback without cone type-selective inhibition mediates "Red-green" color opponency in midget ganglion cells of the primate retina. J Neurosci (2011) 31:1762–72. doi: 10.1523/JNEUROSCI.4385-10.2011
23
DaceyDMLeeBB. The 'Blue-on' opponent pathway in primate retina originates from a distinct bistratified ganglion cell type. Nature (1994) 367:731–5. doi: 10.1038/367731a0
24
WoolLEPackerOSZaidiQDaceyDM. Connectomic identification and three-dimensional color tuning of s-off midget ganglion cells in the primate retina. J Neurosci (2019) 39:7893–909. doi: 10.1523/JNEUROSCI.0778-19.2019
25
BreuningerTPullerCHaverkampSEulerT. Chromatic bipolar cell pathways in the mouse retina. J Neurosci (2011) 31:6504–17. doi: 10.1523/JNEUROSCI.0616-11.2011
26
AwatramaniGBSlaughterMM. Origin of transient and sustained responses in ganglion cells of the retina. J Neurosci (2000) 20:7087–95. doi: 10.1523/JNEUROSCI.20-18-07087.2000
27
DevriesSH. Bipolar cells use kainate and ampa receptors to filter visual information into separate channels. Neuron (2000) 28:847–56. doi: 10.1016/S0896-6273(00)00158-6
28
BorghuisBGLoogerLLTomitaSDembJB. Kainate receptors mediate signaling in both transient and sustained off bipolar cell pathways in mouse retina. J Neurosci (2014) 34:6128–39. doi: 10.1523/JNEUROSCI.4941-13.2014
29
IchinoseTHellmerCB. Differential signalling and glutamate receptor compositions in the off bipolar cell types in the mouse retina. J Physiol (2016) 594:883–94. doi: 10.1113/JP271458
30
SaitoTKanekoA. Ionic mechanisms underlying the responses of off-center bipolar cells in the carp retina. i. studies on responses evoked by light. J Gen Physiol (1983) 81:589–601. doi: 10.1085/jgp.81.4.589
31
PuthusseryTPercivalKAVenkataramaniSGayet-PrimoJGrunertUTaylorWR. Kainate receptors mediate synaptic input to transient and sustained off visual pathways in primate retina. J Neurosci (2014) 34:7611–21. doi: 10.1523/JNEUROSCI.4855-13.2014
32
NakajimaYIwakabeHAkazawaCNawaHShigemotoRMizunoNet al. Molecular characterization of a novel retinal metabotropic glutamate receptor Mglur6 with a high agonist selectivity for l-2-Amino-4-Phosphonobutyrate. J Biol Chem (1993) 268:11868–73. doi: 10.1016/S0021-9258(19)50280-0
33
NawyS. The metabotropic receptor Mglur6 may signal through G(O), but not phosphodiesterase, in retinal bipolar cells. J Neurosci (1999) 19:2938–44. doi: 10.1523/JNEUROSCI.19-08-02938.1999
34
ShenYHeimelJAKamermansMPeacheyNSGreggRGNawyS. A transient receptor potential-like channel mediates synaptic transmission in rod bipolar cells. J Neurosci (2009) 29:6088–93. doi: 10.1523/JNEUROSCI.0132-09.2009
35
KoikeCObaraTUriuYNumataTSanukiRMiyataKet al. Trpm1 is a component of the retinal on bipolar cell transduction channel in the Mglur6 cascade. Proc Natl Acad Sci USA (2010) 107:332–7. doi: 10.1073/pnas.0912730107
36
MorgansCWZhangJJeffreyBGNelsonSMBurkeNSDuvoisinRMet al. Trpm1 is required for the depolarizing light response in retinal on-bipolar cells. Proc Natl Acad Sci USA (2009) 106:19174–8. doi: 10.1073/pnas.0908711106
37
BadenTBerensPFrankeKRoman RosonMBethgeMEulerT. The functional diversity of retinal ganglion cells in the mouse. Nature (2016) 529:345–50. doi: 10.1038/nature16468
38
ShekharKWhitneyIEButrusSPengYRSanesJR. Diversification of multipotential postmitotic mouse retinal ganglion cell precursors into discrete types. Elife (2022) 11:1–36. doi: 10.7554/eLife.73809
39
PengYRShekharKYanWHerrmannDSappingtonABrymanGSet al. Molecular classification and comparative taxonomics of foveal and peripheral cells in primate retina. Cell (2019) 176:1222–1237 E22. doi: 10.1016/j.cell.2019.01.004
40
MasriRAPercivalKAKoizumiAMartinPRGrunertU. Survey of retinal ganglion cell morphology in marmoset. J Comp Neurol (2019) 527:236–58. doi: 10.1002/cne.24157
41
RaviSAhnDGreschnerMChichilniskyEJFieldGD. Pathway-specific asymmetries between on and off visual signals. J Neurosci (2018) 38:9728–40. doi: 10.1523/JNEUROSCI.2008-18.2018
42
NicholsZNirenbergSVictorJ. Interacting linear and nonlinear characteristics produce population coding asymmetries between on and off cells in the retina. J Neurosci (2013) 33:14958–73. doi: 10.1523/JNEUROSCI.1004-13.2013
43
FreedMA. Asymmetry between on and off alpha ganglion cells of mouse retina: Integration of signal and noise from synaptic inputs. J Physiol (2017) 595:6979–91. doi: 10.1113/JP274736
44
ChichilniskyEJKalmarRS. Functional asymmetries in on and off ganglion cells of primate retina. J Neurosci (2002) 22:2737–47. doi: 10.1523/JNEUROSCI.22-07-02737.2002
45
TurnerMHRiekeF. Synaptic rectification controls nonlinear spatial integration of natural visual inputs. Neuron (2016) 90:1257–71. doi: 10.1016/j.neuron.2016.05.006
46
IchinoseTFyk-KolodziejBCohnJ. Roles of on cone bipolar cell subtypes in temporal coding in the mouse retina. J Neurosci (2014) 34:8761–71. doi: 10.1523/JNEUROSCI.3965-13.2014
47
HendricksonAEMovshonJAEggersHMGizziMSBootheRGKiorpesL. Effects of early unilateral blur on the macaque's visual system. ii. anatomical observations. J Neurosci (1987) 7:1327–39. doi: 10.1523/JNEUROSCI.07-05-01327.1987
48
HubermanADWeiWElstrottJStaffordBKFellerMBBarresBA. Genetic identification of an on-off direction-selective retinal ganglion cell subtype reveals a layer-specific subcortical map of posterior motion. Neuron (2009) 62:327–34. doi: 10.1016/j.neuron.2009.04.014
49
HubermanADManuMKochSMSusmanMWLutzABUllianEMet al. Architecture and activity-mediated refinement of axonal projections from a mosaic of genetically identified retinal ganglion cells. Neuron (2008) 59:425–38. doi: 10.1016/j.neuron.2008.07.018
50
SabbaghUGovindaiahGSomaiyaRDHaRVWeiJCGuidoWet al. Diverse gabaergic neurons organize into subtype-specific sublaminae in the ventral lateral geniculate nucleus. J Neurochem (2021) 159:479–97. doi: 10.1111/jnc.15101
51
BrownTMGiasCHatoriMKedingSRSemoMCoffeyPJet al. Melanopsin contributions to irradiance coding in the thalamo-cortical visual system. PloS Biol (2010) 8:E1000558. doi: 10.1371/journal.pbio.1000558
52
EiberCDRahmanASPietersenANJZeaterNDreherBSolomonSGet al. Receptive field properties of koniocellular On/Off neurons in the lateral geniculate nucleus of marmoset monkeys. J Neurosci (2018) 38:10384–98. doi: 10.1523/JNEUROSCI.1679-18.2018
53
Jeczmien-LazurJSOrlowska-FeuerPKustronALewandowskiMH. Short wavelengths contribution to light-induced responses and irradiance coding in the rat dorsal lateral geniculate nucleus - an In vivo electrophysiological approach. Neuroscience (2021) 468:220–34. doi: 10.1016/j.neuroscience.2021.06.016
54
DenmanDJSiegleJHKochCReidRCBlancheTJ. Spatial organization of chromatic pathways in the mouse dorsal lateral geniculate nucleus. J Neurosci (2017) 37:1102–16. doi: 10.1523/JNEUROSCI.1742-16.2016
55
SzelARohlichPCaffeARJuliussonBAguirreGVan VeenT. Unique topographic separation of two spectral classes of cones in the mouse retina. J Comp Neurol (1992) 325:327–42. doi: 10.1002/cne.903250302
56
SzatkoKPKorympidouMMRanYBerensPDalkaraDSchubertTet al. Neural circuits in the mouse retina support color vision in the upper visual field. Nat Commun (2020) 11:3481. doi: 10.1038/s41467-020-17113-8
57
MulyECFitzpatrickD. The morphological basis for binocular and On/Off convergence in tree shrew striate cortex. J Neurosci (1992) 12:1319–34. doi: 10.1523/JNEUROSCI.12-04-01319.1992
58
FitzpatrickD. The functional organization of local circuits in visual cortex: Insights from the study of tree shrew striate cortex. Cereb Cortex (1996) 6:329–41. doi: 10.1093/cercor/6.3.329
59
CallawayEM. Local circuits in primary visual cortex of the macaque monkey. Annu Rev Neurosci (1998) 21:47–74. doi: 10.1146/annurev.neuro.21.1.47
60
BinzeggerTDouglasRJMartinKA. A quantitative map of the circuit of cat primary visual cortex. J Neurosci (2004) 24:8441–53. doi: 10.1523/JNEUROSCI.1400-04.2004
61
MartinezLMWangQReidRCPillaiCAlonsoJMSommerFTet al. Receptive field structure varies with layer in the primary visual cortex. Nat Neurosci (2005) 8:372–9. doi: 10.1038/nn1404
62
NicholasJGMartinRAFuchsPABrownDADiamondMEWeisblatDA. From neuron to brain. 5th Ed. New York, NY, USA: Sinauer Associates (2011).
63
KombanSJKremkowJJinJWangYLashgariRLiXet al. Neuronal and perceptual differences in the temporal processing of darks and lights. Neuron (2014) 82:224–34. doi: 10.1016/j.neuron.2014.02.020
64
JinJWangYLashgariRSwadlowHAAlonsoJM. Faster thalamocortical processing for dark than light visual targets. J Neurosci (2011) 31:17471–9. doi: 10.1523/JNEUROSCI.2456-11.2011
65
MazadeRJinJPonsCAlonsoJM. Functional specialization of on and off cortical pathways for global-slow and local-fast vision. Cell Rep (2019) 27:2881–2894 E5. doi: 10.1016/j.celrep.2019.05.007
66
WilliamsBDel RosarioJMuzzuTPeelmanKColettaSBichlerEKet al. Spatial modulation of dark versus bright stimulus responses in the mouse visual system. Curr Biol (2021) 31:4172–4179 E6. doi: 10.1016/j.cub.2021.06.094
67
KremkowJJinJKombanSJWangYLashgariRLiXet al. Neuronal nonlinearity explains greater visual spatial resolution for darks than lights. Proc Natl Acad Sci USA (2014) 111:3170–5. doi: 10.1073/pnas.1310442111
68
PonsCMazadeRJinJDulMWZaidiQAlonsoJM. Neuronal mechanisms underlying differences in spatial resolution between darks and lights in human vision. J Vis (2017) 17:5. doi: 10.1167/17.14.5
69
JinJZWengCYehCIGordonJARuthazerESStrykerMPet al. On and off domains of geniculate afferents in cat primary visual cortex. Nat Neurosci (2008) 11:88–94. doi: 10.1038/nn2029
70
XingDYehCIShapleyRM. Generation of black-dominant responses in V1 cortex. J Neurosci (2010) 30:13504–12. doi: 10.1523/JNEUROSCI.2473-10.2010
71
YehCIXingDShapleyRM. "Black" responses dominate macaque primary visual cortex V1. J Neurosci (2009) 29:11753–60. doi: 10.1523/JNEUROSCI.1991-09.2009
72
LiuKYaoH. Contrast-dependent off-dominance in cat primary visual cortex facilitates discrimination of stimuli with natural contrast statistics. Eur J Neurosci (2014) 39:2060–70. doi: 10.1111/ejn.12567
73
WangLSarnaikRRangarajanKLiuXCangJ. Visual receptive field properties of neurons in the superficial superior colliculus of the mouse. J Neurosci (2010) 30:16573–84. doi: 10.1523/JNEUROSCI.3305-10.2010
74
Rivlin-EtzionMZhouKWeiWElstrottJNguyenPLBarresBAet al. Transgenic mice reveal unexpected diversity of on-off direction-selective retinal ganglion cell subtypes and brain structures involved in motion processing. J Neurosci (2011) 31:8760–9. doi: 10.1523/JNEUROSCI.0564-11.2011
75
SabbahSBergDPapendorpCBriggmanKLBersonDM. A cre mouse line for probing irradiance- and direction-encoding retinal networks. Eneuro (2017) 4:1–21. doi: 10.1523/ENEURO.0065-17.2017
76
ChandrasekaranARShahRDCrairMC. Developmental homeostasis of mouse retinocollicular synapses. J Neurosci (2007) 27:1746–55. doi: 10.1523/JNEUROSCI.4383-06.2007
77
De FranceschiGSolomonSG. Visual response properties of neurons in the superficial layers of the superior colliculus of awake mouse. J Physiol (2018) 596:6307–32. doi: 10.1113/JP276964
78
KanedaKIsaT. Gabaergic mechanisms for shaping transient visual responses in the mouse superior colliculus. Neuroscience (2013) 235:129–40. doi: 10.1016/j.neuroscience.2012.12.061
79
HubelDHWieselTN. Receptive fields of single neurones in the cat's striate cortex. J Physiol (1959) 148:574–91. doi: 10.1113/jphysiol.1959.sp006308
80
HubelDHWieselTN. Receptive fields, binocular interaction and functional architecture in the cat's visual cortex. J Physiol (1962) 160:106–54. doi: 10.1113/jphysiol.1962.sp006837
81
LeeKSHuangXFitzpatrickD. Topology of on and off inputs in visual cortex enables an invariant columnar architecture. Nature (2016) 533:90–4. doi: 10.1038/nature17941
82
VnekNRamsdenBMHungCPGoldman-RakicPSRoeAW. Optical imaging of functional domains in the cortex of the awake and behaving monkey. Proc Natl Acad Sci USA (1999) 96:4057–60. doi: 10.1073/pnas.96.7.4057
83
ChenGWangFGoreJCRoeAW. Layer-specific bold activation in awake monkey V1 revealed by ultra-high spatial resolution functional magnetic resonance imaging. Neuroimage (2013) 64:147–55. doi: 10.1016/j.neuroimage.2012.08.060
84
HumphreyALNortonTT. Topographic organization of the orientation column system in the striate cortex of the tree shrew (Tupaia glis). i. microelectrode recording. J Comp Neurol (1980) 192:531–47. doi: 10.1002/cne.901920311
85
BoskingWHZhangYSchofieldBFitzpatrickD. Orientation selectivity and the arrangement of horizontal connections in tree shrew striate cortex. J Neurosci (1997) 17:2112–27. doi: 10.1523/JNEUROSCI.17-06-02112.1997
86
LawMIZahsKRStrykerMP. Organization of primary visual cortex (Area 17) in the ferret. J Comp Neurol (1988) 278:157–80. doi: 10.1002/cne.902780202
87
VeitJBhattacharyyaAKretzRRainerG. On the relation between receptive field structure and stimulus selectivity in the tree shrew primary visual cortex. Cereb Cortex (2014) 24:2761–71. doi: 10.1093/cercor/bht133
88
WangYJinJKremkowJLashgariRKombanSJAlonsoJM. Columnar organization of spatial phase in visual cortex. Nat Neurosci (2015) 18:97–103. doi: 10.1038/nn.3878
89
KremkowJJinJWangYAlonsoJM. Principles underlying sensory map topography in primary visual cortex. Nature (2016) 533:52–7. doi: 10.1038/nature17936
90
TringEDuanKKRingachDL. On/Off domains shape receptive field structure in mouse visual cortex. Nat Commun (2022) 13:2466. doi: 10.1038/s41467-022-29999-7
91
GiolliRAPopeJE. The anatomical organization of the visual system of the rabbit. Doc Ophthalmol (1971) 30:9–31. doi: 10.1007/BF00142517
92
NathASchwartzGW. Electrical synapses convey orientation selectivity in the mouse retina. Nat Commun (2017) 8:2025. doi: 10.1038/s41467-017-01980-9
93
NathASchwartzGW. Cardinal orientation selectivity is represented by two distinct ganglion cell types in mouse retina. J Neurosci (2016) 36:3208–21. doi: 10.1523/JNEUROSCI.4554-15.2016
94
VenkataramaniSTaylorWR. Synaptic mechanisms generating orientation selectivity in the on pathway of the rabbit retina. J Neurosci (2016) 36:3336–49. doi: 10.1523/JNEUROSCI.1432-15.2016
95
YoneharaKFarrowKGhanemAHillierDBalintKTeixeiraMet al. The first stage of cardinal direction selectivity is localized to the dendrites of retinal ganglion cells. Neuron (2013) 79:1078–85. doi: 10.1016/j.neuron.2013.08.005
96
LipinMYTaylorWRSmithRG. Inhibitory input to the direction-selective ganglion cell is saturated At low contrast. J Neurophysiol (2015) 114:927–41. doi: 10.1152/jn.00413.2015
97
MaximovaEGovardovskiVMaximovPMaximovV. Spectral sensitivity of direction-selective ganglion cells in the fish retina. Ann N Y Acad Sci (2005) 1048:433–4. doi: 10.1196/annals.1342.056
98
EulerTDetwilerPBDenkW. Directionally selective calcium signals in dendrites of starburst amacrine cells. Nature (2002) 418:845–52. doi: 10.1038/nature00931
99
LeeSZhouZJ. The synaptic mechanism of direction selectivity in distal processes of starburst amacrine cells. Neuron (2006) 51:787–99. doi: 10.1016/j.neuron.2006.08.007
100
KimYJPetersonBBCrookJDJooHRWuJPullerCet al. Origins of direction selectivity in the primate retina. Nat Commun (2022) 13:2862. doi: 10.1038/s41467-022-30405-5
101
PattersonSSBembryBNMazzaferriMANeitzMRiekeFSoetedjoRet al. Conserved circuits for direction selectivity in the primate retina. Curr Biol (2022) 32(11):2529–38.e4. doi: 10.1016/j.cub.2022.04.056
102
DhandeOSStaffordBKFrankeKEl-DanafRPercivalKAPhanAHet al. Molecular fingerprinting of on-off direction-selective retinal ganglion cells across species and relevance to primate visual circuits. J Neurosci (2019) 39:78–95. doi: 10.1523/JNEUROSCI.1784-18.2018
103
ReinhardKMunchTA. Visual properties of human retinal ganglion cells. PloS One (2021) 16:E0246952. doi: 10.1371/journal.pone.0246952
104
InayatSBarchiniJChenHFengLLiuXCangJ. Neurons in the most superficial lamina of the mouse superior colliculus are highly selective for stimulus direction. J Neurosci (2015) 35:7992–8003. doi: 10.1523/JNEUROSCI.0173-15.2015
105
HorwitzGDNewsomeWT. Target selection for saccadic eye movements: Direction-selective visual responses in the superior colliculus. J Neurophysiol (2001) 86:2527–42. doi: 10.1152/jn.2001.86.5.2527
106
DamjanovicIMaximovPVAliperATZaichikovaAAGacicZMaximovaEM. Putative targets of direction-selective retinal ganglion cells in the tectum opticum of cyprinid fish. Brain Res (2019) 1708:20–6. doi: 10.1016/j.brainres.2018.12.006
107
KanjhanRSivyerB. Two types of on direction-selective ganglion cells in rabbit retina. Neurosci Lett (2010) 483:105–9. doi: 10.1016/j.neulet.2010.07.071
108
WyattHJDawNW. Directionally sensitive ganglion cells in the rabbit retina: Specificity for stimulus direction, size, and speed. J Neurophysiol (1975) 38:613–26. doi: 10.1152/jn.1975.38.3.613
109
TsvillingVDonchinOShamirMSegevR. Archer fish fast hunting maneuver may be guided by directionally selective retinal ganglion cells. Eur J Neurosci (2012) 35:436–44. doi: 10.1111/j.1460-9568.2011.07971.x
110
MaximovVMaximovaEMaximovP. Direction selectivity in the goldfish tectum revisited. Ann NY Acad Sci (2005) 1048:198–205. doi: 10.1196/annals.1342.018
111
SchlegelDKNeuhaussSCF. Chapter 3 - the larval visual system and behavioral responses to visual stimuli. In: GerlaiRT, editor. Behavioral and neural genetics of zebrafish. Cambridge, MA, USA: Academic Press (2020).
112
CameronDJRassamdanaFTamPDangKYanezCGhaemmaghamiSet al. The optokinetic response as a quantitative measure of visual acuity in zebrafish. J Vis Exp (2013) (80):50832. doi: 10.3791/50832
113
SugitaYMiuraKArakiFFurukawaTKawanoK. Contributions of retinal direction-selective ganglion cells to optokinetic responses in mice. Eur J Neurosci (2013) 38:2823–31. doi: 10.1111/ejn.12284
114
PruskyGTAlamNMBeekmanSDouglasRM. Rapid quantification of adult and developing mouse spatial vision using a virtual optomotor system. Invest Ophthalmol Vis Sci (2004) 45:4611–6. doi: 10.1167/iovs.04-0541
115
Buttner-EnneverJA. Neuroanatomy of the oculomotor system. preface. Prog Brain Res (2006) 151:Vii–Viii. doi: 10.1016/S0079-6123(05)51019-7
116
GiolliRABlanksRHLuiF. The accessory optic system: Basic organization with an update on connectivity, neurochemistry, and function. Prog Brain Res (2006) 151:407–40. doi: 10.1016/S0079-6123(05)51013-6
117
PortuguesRFeiersteinCEEngertFOrgerMB. Whole-brain activity maps reveal stereotyped, distributed networks for visuomotor behavior. Neuron (2014) 81:1328–43. doi: 10.1016/j.neuron.2014.01.019
118
MaceEMontaldoGTrenholmSCowanCBrignallAUrbanAet al. Whole-brain functional ultrasound imaging reveals brain modules for visuomotor integration. Neuron (2018) 100:1241–51.E7. doi: 10.1016/j.neuron.2018.11.031
119
AllwardtBALallABBrockerhoffSEDowlingJE. Synapse formation is arrested in retinal photoreceptors of the zebrafish <Em>Nrc</Em> mutant. J Of Neurosci (2001) 21:2330–42. doi: 10.1523/JNEUROSCI.21-07-02330.2001
120
EmranFRihelJAdolphARWongKYKravesSDowlingJE. Off ganglion cells cannot drive the optokinetic reflex in zebrafish. Proc Of Natl Acad Of Sci (2007) 104:19126–31. doi: 10.1073/pnas.0709337104
121
AungMHHoganKMazadeREParkHNSidhuCSIuvonePMet al. On than off pathway disruption leads to greater deficits in visual function and retinal dopamine signaling. Exp Eye Res (2022) 220:109091. doi: 10.1016/j.exer.2022.109091
122
JolySGuzik-KornackaASchwabMEPernetV. New mouse retinal stroke model reveals direction-selective circuit damage linked to permanent optokinetic response loss. Invest Ophthalmol Vis Sci (2014) 55:4476–89. doi: 10.1167/iovs.14-14521
123
JoeschMSchnellBRaghuSVReiffDFBorstA. On and off pathways in drosophila motion vision. Nature (2010) 468:300–4. doi: 10.1038/nature09545
124
YilmazMMeisterM. Rapid innate defensive responses of mice to looming visual stimuli. Curr Biol (2013) 23:2011–5. doi: 10.1016/j.cub.2013.08.015
125
De FranceschiGVivattanasarnTSaleemABSolomonSG. Vision guides selection of freeze or flight defense strategies in mice. Curr Biol (2016) 26:2150–4. doi: 10.1016/j.cub.2016.06.006
126
MunchTADa SilveiraRASiegertSVineyTJAwatramaniGBRoskaB. Approach sensitivity in the retina processed by a multifunctional neural circuit. Nat Neurosci (2009) 12:1308–16. doi: 10.1038/nn.2389
127
KimTShenNHsiangJCJohnsonKPKerschensteinerD. Dendritic and parallel processing of visual threats in the retina control defensive responses. Sci Adv (2020) 6:1–11. doi: 10.1126/sciadv.abc9920
128
WangFLiEDeLWuQZhangY. Off-transient alpha rgcs mediate looming triggered innate defensive response. Curr Biol (2021) 31:2263–2273 E3. doi: 10.1016/j.cub.2021.03.025
129
KlapoetkeNCNernAPeekMYRogersEMBreadsPRubinGMet al. Ultra-selective looming detection from radial motion opponency. Nature (2017) 551:237–41. doi: 10.1038/nature24626
130
SanesJRZipurskySL. Design principles of insect and vertebrate visual systems. Neuron (2010) 66:15–36. doi: 10.1016/j.neuron.2010.01.018
131
GabbianiFKrappHGKochCLaurentG. Multiplicative computation in a visual neuron sensitive to looming. Nature (2002) 420:320–4. doi: 10.1038/nature01190
132
GuestBBGrayJR. Responses of a looming-sensitive neuron to compound and paired object approaches. J Neurophysiol (2006) 95:1428–41. doi: 10.1152/jn.01037.2005
133
Rivlin-EtzionMGrimesWNRiekeF. Flexible neural hardware supports dynamic computations in retina. Trends Neurosci (2018) 41:224–37. doi: 10.1016/j.tins.2018.01.009
134
VlasitsALBosRMorrieRDFortunyCFlanneryJGFellerMBet al. Visual stimulation switches the polarity of excitatory input to starburst amacrine cells. Neuron (2014) 83:1172–84. doi: 10.1016/j.neuron.2014.07.037
135
Rivlin-EtzionMWeiWFellerMB. Visual stimulation reverses the directional preference of direction-selective retinal ganglion cells. Neuron (2012) 76:518–25. doi: 10.1016/j.neuron.2012.08.041
136
AnkriLEzra-TsurEMaimonSRKaushanskyNRivlin-EtzionM. Antagonistic center-surround mechanisms for direction selectivity in the retina. Cell Rep (2020) 31:107608. doi: 10.1016/j.celrep.2020.107608
137
Tikidji-HamburyanAReinhardKSeitterHHovhannisyanAProcykCAAllenAEet al. Retinal output changes qualitatively with every change in ambient illuminance. Nat Neurosci (2015) 18:66–74. doi: 10.1038/nn.3891
138
PearsonJTKerschensteinerD. Ambient illumination switches contrast preference of specific retinal processing streams. J Neurophysiol (2015) 114:540–50. doi: 10.1152/jn.00360.2015
139
SagdullaevBTMccallMA. Stimulus size and intensity alter fundamental receptive-field properties of mouse retinal ganglion cells In vivo. Vis Neurosci (2005) 22:649–59. doi: 10.1017/S0952523805225142
140
FarrowKTeixeiraMSzikraTVineyTJBalintKYoneharaKet al. Ambient illumination toggles a neuronal circuit switch in the retina and visual perception At cone threshold. Neuron (2013) 78:325–38. doi: 10.1016/j.neuron.2013.02.014
141
PangJJGaoFWuSM. Ionotropic glutamate receptors mediate off responses in light-adapted on bipolar cells. Vision Res (2012) 68:48–58. doi: 10.1016/j.visres.2012.07.012
142
HoshiHLiuWLMasseySCMillsSL. On inputs to the off layer: Bipolar cells that break the stratification rules of the retina. J Neurosci (2009) 29:8875–83. doi: 10.1523/JNEUROSCI.0912-09.2009
143
GeffenMNDe VriesSEMeisterM. Retinal ganglion cells can rapidly change polarity from off to on. PloS Biol (2007) 5:E65. doi:Â 10.1371/journal.pbio.0050065
144
SivyerBTomlinsonATaylorWR. Simulated saccadic stimuli suppress on-type direction-selective retinal ganglion cells Via glycinergic inhibition. J Neurosci (2019) 39:4312–22. doi: 10.1523/JNEUROSCI.3066-18.2019
145
LiangZFreedMA. The on pathway rectifies the off pathway of the mammalian retina. J Neurosci (2010) 30:5533–43. doi: 10.1523/JNEUROSCI.4733-09.2010
146
LeonhardtAAmmerGMeierMSerbeEBahlABorstA. Asymmetry of drosophila on and off motion detectors enhances real-world velocity estimation. Nat Neurosci (2016) 19:706–15. doi: 10.1038/nn.4262
147
SievingPAMurayamaKNaarendorpF. Push-pull model of the primate photopic electroretinogram: A role for hyperpolarizing neurons in shaping the b-wave. Vis Neurosci (1994) 11:519–32. doi: 10.1017/S0952523800002431
148
FarrellDF. Unilateral retinitis pigmentosa and cone-rod dystrophy. Clin Ophthalmol (2009) 3:263–70. doi: 10.2147/OPTH.S5130
149
MiyakeYYagasakiKHoriguchiMKawaseY. On- and off-responses in photopic electroretinogram in complete and incomplete types of congenital stationary night blindness. Jpn J Ophthalmol (1987) 31:81–7.
150
TsangSHSharmaT. Congenital stationary night blindness. Adv Exp Med Biol (2018) 1085:61–4. doi: 10.1007/978-3-319-95046-4_13
151
Miraldi UtzVPfeiferWLongmuirSQOlsonRJWangKDrackAV. Presentation of Trpm1-associated congenital stationary night blindness in children. JAMA Ophthalmol (2018) 136:389–98. doi: 10.1001/jamaophthalmol.2018.0185
Summary
Keywords
retina, bipolar cell, parallel processing, visual system, primary visual cortex, superior colliculus
Citation
Ichinose T and Habib S (2022) On and off signaling pathways in the retina and the visual system. Front. Ophthalmol. 2:989002. doi: 10.3389/fopht.2022.989002
Received
07 July 2022
Accepted
05 August 2022
Published
26 August 2022
Volume
2 - 2022
Edited by
Genea Edwards, University of Missouri–Kansas City, United States
Reviewed by
Benedetto Falsini, UniversitĂ Cattolica del Sacro Cuore, Italy; Judith Mosinger Ogilvie, Saint Louis University, United States
Updates
Copyright
© 2022 Ichinose and Habib.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Tomomi Ichinose, tichinos@med.wayne.edu
This article was submitted to Retina, a section of the journal Frontiers in Ophthalmology
Disclaimer
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