Investigating the prevalence of three medically important pathogens in Ixodes pacificus from southern Oregon

Andrew Partin^1*Emilio DeBess²Phillip Spinks³Michael Yabsley^4,5,6Kayla Garrett^4,5,6James Clover⁷Geoffrey Taylor¹

• ¹Jackson County Vector Control District, Central Point, United States
• ²Oregon Health Authority, Salem, Oregon, United States
• ³Placer Mosquito and Vector Control District, Roseville, United States
• ⁴Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States
• ⁵Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia, United States
• ⁶Center for the Ecology of Infectious Diseases, Odum School of Ecology, University of Georgia, Athens, United States
• ⁷Biological Consultant, Grants Pass, United States

In the far western United States of America, Ixodes pacificus is the primary vector of several pathogens of public health and veterinary importance including the Lyme disease spirochete Borrelia burgdorferi sensu lato (s.l.), as well as Borrelia miyamotoi and Anaplasma phagocytophilum. Ixodes pacificus is common in southern Oregon yet there are few published studies on the distribution of tick-borne pathogens in this region. Using real-time quantitative PCR, we assessed the prevalence of B. burgdorferi s.l., B. miyamotoi, and A. phagocytophilum among 2,463 unfed I. pacificus adults and nymphs combined into 260 pools (131 nymph, 129 adult) with nearly equal numbers of each life stage from 12 locations in Jackson County, Oregon.In our study, 27.9% (36/129) and 29.8% (39/131) of adult and nymph pools, respectively, tested positive for at least a single pathogen. Nymph pools had a higher pool positivity rate (PPR) for B. burgdorferi s.l. with 15.3% (20/131) testing positive compared to 3.1% (4/129) of adult pools.Nymph pools also had a higher minimum infection rate (MIR) and maximum-likelihood estimate of pooled prevalence (EPP) for B. burgdorferi s.l. than adults. Interestingly, the prevalence of B. burgdorferi s.l. varied greatly in nymph pools across collection sites (0-70%). PPR of B. miyamotoi was 21.7% (28/129) for adults and 12.2% (16/131) for nymphs, making it the most frequently detected pathogen in adult pools and most detected pathogen overall. Anaplasma phagocytophilum was the least frequently detected pathogen overall with a PPR of 3.1% (4/129) and 2.3% (3/131) for adults and nymphs, respectively. These findings underscore the importance of continued surveillance, pathogen testing, and public education regarding ticks in areas such as southern Oregon where I. pacificus is common but little research has been done.