Original Research ARTICLE
Potential of Manuka Honey as a natural polyelectrolyte to develop biomimetic nanostructured meshes with antimicrobial properties
- 1Ulster University, United Kingdom
- 2Politecnico di Torino, Italy
- 3Department of Pharmaceutical Sciences, University of Eastern Piedmont, Italy
- 4University of Leeds, United Kingdom
- 5Newcastle University, United Kingdom
The use of antibiotics has been the cornerstone to prevent bacterial infections; however, the emergency of antibiotic resistant bacteria is still an open challenge. This work aimed to develop a delivery system for treating soft tissue infections for: (1) reducing the released antimicrobial amount, preventing drug-related systemic side effects, (2) re-discovering the beneficial effects of naturally-derived agents, and (3) preserving the substrate functional properties. For the first time Manuka honey (MH) was proposed as polyelectrolyte within the layer-by-layer (LbL) assembly. Biomimetic electrospun poly(ε-caprolactone) meshes were treated via LbL assembly to obtain a multilayered nanocoating, consisting of MH as polyanion and poly-(allylamine-hydrochloride) as polycation. Physico-chemical characterisation demonstrated the successful nano-coating formation. Different cell lines (Human immortalized and primary skin fibroblasts, and primary endothelial cells) confirmed positively the membranes cytocompatibility, while bacterial tests using Gram-negative and Gram-positive bacteria demonstrated the antimicrobial MH activity was dependent on the concentration used and strains tested.
Keywords: Layer - by - layer assembly, Manuka honey, Nanofunctionalization, Soft tissue regeneration, Electrospinning
Received: 01 Jul 2019;
Accepted: 06 Nov 2019.
Copyright: © 2019 Mancuso, Tonda-Turo, Ceresa, Pensabene, Connell, Fracchia and Gentile. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Dr. Elena Mancuso, Ulster University, Coleraine, BT52 1SA, United Kingdom, firstname.lastname@example.org
Dr. Piergiorgio Gentile, Newcastle University, Newcastle upon Tyne, United Kingdom, Piergiorgio.email@example.com