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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Cell Dev. Biol. | doi: 10.3389/fcell.2019.00173

The microtubule-depolymerizing kinesin-13 Klp10A is enriched in the transition zone of the ciliary structures of Drosophila melanogaster.

 Giuliano Callaini1*, Maria G. Riparbelli1 and  Veronica Persico1
  • 1University of Siena, Italy

The precursor of the flagellar axoneme is already present in the primary spermatocytes of Drosophila melanogaster. During spermatogenesis each primary spermatocyte shows a centriole pair that moves to the cell membrane and organizes an axoneme-based structure, the cilium-like region (CLR). The CLRs persist through the meiotic divisions and are inherited by young spermatids. During spermatid differentiation the ciliary caps elongate giving rise to the sperm axoneme. Mutations in Klp10A, a kinesin-13 of Drosophila, results in defects of centriole/CLR organization in spermatocytes and of ciliary cap assembly in elongating spermatids. Reduced Klp10A expression also results in strong structural defects of sensory type I neurons. We show, here, that this protein displays a peculiar localization during male gametogenesis. The Klp10A signal is first detected at the distal ends of the centrioles when they dock to the plasma membrane of young primary spermatocytes. At the onset of the first meiotic prometaphase, when the CLRs reach their full size, Klp10A is enriched in a distinct narrow area at the distal end of the centrioles and persists in elongating spermatids at the base of the ciliary cap. We conclude that Klp10A could be a core component of the ciliary transition zone in Drosophila.

Keywords: Gametogenesis,, Klp10A, Ciliary structures, transition zone, Centrioles, Drosophila

Received: 31 May 2019; Accepted: 07 Aug 2019.

Edited by:

Tomer Avidor-Reiss, University of Toledo, United States

Reviewed by:

Bénédicte DURAND, Université Claude Bernard Lyon 1, France
Maurice Kernan, Stony Brook University, United States  

Copyright: © 2019 Callaini, Riparbelli and Persico. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Giuliano Callaini, University of Siena, Siena, Italy, callaini@unisi.it