Frontiers journals are at the top of citation and impact metrics

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Behav. Neurosci. | doi: 10.3389/fnbeh.2019.00240

LPS-induced systemic neonatal inflammation: blockage of P2X7R by BBG decreases mortality on rat pups and oxidative stress in hippocampus of adult rats

  • 1Department of Physiology, Universidade Federal de São Paulo, Brazil
  • 2Department of Biochemistry, Universidade Federal de São Paulo, Brazil
  • 3Department of Physiology, Federal University of São Paulo, Brazil
  • 4D’Or Institute for Research and Education, Brazil
  • 5Department of Pediatry, Federal University of São Paulo, Brazil

Neonatal lipopolysaccharide (LPS) exposure-induced brain inflammation has been associated to neuronal injury and facilitates the development of models of neurological disorders in adult rats. The P2X7 receptor (P2X7R) plays a fundamental role in the onset and maintenance of the inflammatory cascade. Brilliant blue G (BBG), a P2X7R antagonist, has been shown to effectively promote neuroinflammatory protection. Here, we have investigated the long-term effects of the neonatal systemic inflammation on hippocampal oxidative stress, anxiety behavior and pain sensitivity in adulthood. We hypothesized that P2X7R blockade is able to modulate the effects of inflammation on these variables. Male and female rat pups received LPS and/or BBG solution intraperitoneally on the first, third, fifth and seventh postnatal days. The survival rate and body weight were evaluated during the experimental procedures. The animals were submitted to behavioral tests for anxiety (elevated plus maze) and nociception (hot-plate and tail flick) and the oxidative stress was measured by superoxide production in the dentate gyrus of the hippocampus using dihydroethidium (DHE) probe. BBG increased the survival rate in lipopolysaccharide treated rats. No significant differences were found regarding anxiety behavior and pain sensitivity between the experimental groups. Systemic neonatal inflammation lead to a higher production of superoxide anion in the dentate gyrus of the hippocampus in adulthood and BBG inhibited that effect. Our data suggest that blocking the activation of the P2X7R during neonatal systemic inflammation may have a potential neuroprotective effect in adulthood.

Keywords: neonate, brillant blue G, Lipopolysacaride, Inflammation, P2X7 receptor antagonist

Received: 14 Jan 2019; Accepted: 24 Sep 2019.

Copyright: © 2019 Silva, Calió, Mosini, Pires, Rêgo, Mello and Leslie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Ana Teresa F. Leslie, Federal University of São Paulo, Department of Pediatry, São Paulo, São Paulo, Brazil,