In the published article, there were errors in the legend for Figure 5 as published. “ε” symbol was mistakenly used for “≥” and the color scheme for high and low synchrony was incorrect. The correct color scheme is purple for “high” synchrony and yellow for “low” synchrony. The corrected legend appears below.
“The effect of BIC, AP-5, and CNQX on synchronized neural network activity within a 3D neuron-astrocyte co-culture. (A) The heat map illustrates the average synchrony value per array within (bottom, middle 1, middle 2, and top) and between cross sections before and after the sequential addition of BIC, AP-5, and CNQX. (B) Overlay bar graph compares the average number of synchronized networks (or edges) detected within the 3D culture, independent of the electrode's position, across treatment conditions (e.g., baseline, BIC, AP-5+BIC, and CNQX+AP-5+BIC). Data was normalized to the total edges identified during BIC treatment (see Results for rationale). Edge activity has been categorized by the degree of synchrony: “high synchrony” has a 1-SPIKE distance ≥ 0.40 (purple), “low synchrony” has a 1-SPIKE distance < 0.40 (yellow), or inactive electrodes (white). Data is shown as mean ± SEM. (C) Scatter plot illustrates the shift in synchrony value for an active edge (dot) detected in BIC treatment (Reference, black line), its value before (e.g., baseline,), and after (e.g., AP-5 and CNQX) BIC treatment. The line of best fit was determined based on all available edges, categorized by the location within (i) or between (ii) cross sections, and the slope and intercept reported for each antagonist in brackets. (D) Scatter plot illustrates the shift in synchrony value for an active edge detected in BIC treatment (Reference, black line) based on its cross sectional position (e.g., bottom, middle 1, middle 2, and top graph) for baseline (i), AP-5 (ii), and CNQX (iii). The slope and intercept are reported based on the line of best fit for each cross section. Electrode data has been aggregated for 9 3D MEAs.”
In the published article, there was an error in Figure 2 as published. For panel B, the graph shown in burst per minute for the middle 2 cross section is incorrect. The corrected Figure 2 and its original caption appear below.
Figure 2
In the published article, Supplementary Table 1 was mistakenly not included in the publication.
The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Statements
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Summary
Keywords
3D multi-electrode array, microelectrode array, neural networks, electrophysiology, 3D culture, hiPSC, collagen
Citation
Lam D, Enright HA, Cadena J, George VK, Soscia DA, Tooker AC, Triplett M, Peters SKG, Karande P, Ladd A, Bogguri C, Wheeler EK and Fischer NO (2023) Corrigendum: Spatiotemporal analysis of 3D human iPSC-derived neural networks using a 3D multi-electrode array. Front. Cell. Neurosci. 17:1340688. doi: 10.3389/fncel.2023.1340688
Received
18 November 2023
Accepted
22 November 2023
Published
07 December 2023
Volume
17 - 2023
Edited and reviewed by
Emilio Carbone, University of Turin, Italy
Updates
Copyright
© 2023 Lam, Enright, Cadena, George, Soscia, Tooker, Triplett, Peters, Karande, Ladd, Bogguri, Wheeler and Fischer.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Nicholas O. Fischer fischer29@llnl.govDoris Lam lam31@llnl.gov
Disclaimer
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.