Original Research ARTICLE
Construction and Investigation of a lncRNA-associated ceRNA Regulatory Network in Cholangiocarcinoma
- 1Peking Union Medical College Hospital (CAMS), China
Background: As a type of malignant tumor commonly found in the bile duct, cholangiocarcinoma (CCA) has a poor prognosis. Long non-coding RNA (lncRNA) has recently drawn increasing attention because it functions as a competing endogenous RNA (ceRNA) to hinder miRNA functions that participate in posttranscriptional regulatory networks in tumors. Therefore, to investigate the mechanisms of CCA carcinogenesis and to enhance treatment efficiency, the expression profiles, including lncRNA, miRNA and mRNA data, were comprehensively integrated and analyzed in this study.
Methods: A comprehensive comparison was performed on the RNA-sequencing and miRNA profiles data of 36 CCA tissues and 9 normal tissues from The Cancer Genome Atlas (TCGA) database. Then, a dysregulated lncRNA-related ceRNA network was constructed by using four public databases.
Results: In total, 1,410 lncRNAs, 64 miRNAs, and 3,494 mRNAs appeared as genes that were aberrantly expressed in CCA. Then, a dysregulated ceRNA network related to the lncRNAs was constructed. The network included 116 lncRNAs, 13 miRNAs and 60 mRNAs specific to CCA. The survival analysis showed that, among them, 26 lncRNAs, 3 miRNAs, and 13 mRNAs were prognostic biomarkers for patients with CCA. Finally, three mRNAs were selected for validation of their expression levels in the Gene Expression Omnibus (GEO) database. The results showed that the expression of those genes was highly consistent between the TCGA and GEO databases.
Conclusions: The findings in this study provide a better understanding of the ceRNA network involved in CCA biology and lay a solid foundation for improving CCA diagnosis and prognosis.
Keywords: long non-coding RNA, microRNA, Cholangiocarcinoma, prognosis, competing endogenous RNA network
Received: 20 Mar 2019;
Accepted: 02 Jul 2019.
Edited by:Cesar Wong, Hong Kong Polytechnic University, Hong Kong
Reviewed by:Daniele Vergara, University of Salento, Italy
Hua Zhang, Guangdong Medical University, China
Copyright: © 2019 Long, Xiong, Mao, Zhang, Bai, Lin, Xu, Chen and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Prof. Shuguang Chen, Peking Union Medical College Hospital (CAMS), Beijing, 100730, Beijing Municipality, China, email@example.com
Dr. Haitao Zhao, Peking Union Medical College Hospital (CAMS), Beijing, 100730, Beijing Municipality, China, firstname.lastname@example.org