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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Oncol. | doi: 10.3389/fonc.2019.01129

Circulating exosomal miR-150-5p and miR-99b-5p were association with colorectal cancer

  • 1Shandong Cancer Hospital, Shandong University, China
  • 2School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, China

Background: Circulating exosomal miRNAs are potential non-invasive biomarkers for colorectal cancer. The present study aimed to validate the novel sensitive and specific exosomal miRNA biomarkers for diagnosing colorectal cancer (CRC).
Patients and methods: Exosomes isolated from the serum of CRC patients and healthy donors by ultracentrifugation were characterized using TEM, qNano, and immunoblotting. The exosomes from 2 healthy donors and 4 CRC patients were subjected to RNA isolation and miRNA sequencing. The differently expressed miRNAs from 165 primary CRC patients and 153 healthy donors were substantiated by RT-qPCR.
Results: The RNA-sequence data analysis revealed that 29 exosomal miRNAs (20 downregulated and 9 upregulated) with >2-fold difference between CRC patients and healthy donors were selected. The serum exosomal miR-99b-5p and miR-150-5p levels were significantly downregulated in CRC patients as compared to healthy donors (p<0.0001 and p<0.0001, respectively) and benign disease (p=0.009 and p<0.0001, respectively). The expression levels of exosomal miR-99b-5p and miR-150-5p were significantly decreased in early CRC patients as compared to healthy donors (p<0.0001 and p<0.0001, respectively). The expression levels of exosomal miR-99b-5p and miR-150-5p were significantly increased postoperatively (p=0.0058 and p<0.0001, respectively).
Conclusions: The present study demonstrated that serum exosomal miRNAs are promising, sensitive, specific, and non-invasive diagnostic biomarkers for CRC.

Keywords: Exosomes, colorectal cancer, MiR-99b-5p, miR-150-5p, diagnosis

Received: 16 Jul 2019; Accepted: 09 Oct 2019.

Copyright: © 2019 Li, Yajing, Xingguo, limin, Youyong and Xianrang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Xie Li, Shandong Cancer Hospital, Shandong University, Jinan, China, l_xie2001@126.com