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CORRECTION article

Front. Parasitol., 10 October 2025

Sec. Molecular Cellular Parasitology

Volume 4 - 2025 | https://doi.org/10.3389/fpara.2025.1711595

Correction: In vitro co-culture model of Trichomonas vaginalis, Candida albicans, and Lactobacillus crispatus: a system for assessing antimicrobial activity and microorganism interactions in vaginitis

Fernanda Gomes CardosoFernanda Gomes CardosoLuisa Trindade dos SantosLuisa Trindade dos SantosSaulo Almeida MenezesSaulo Almeida MenezesGraziela Vargas RigoGraziela Vargas RigoTiana Tasca*Tiana Tasca*
  • Faculdade de Farmácia and Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

In the published article, there was a mistake in Figure 3 and Figure 5. Figure 3 appeared in place of Figure 5 and vice versa. The corrected figures appear below.

Figure 3
Bar graphs labeled A and B show hemolysis percentages. Graph A compares five groups, including positive control, with varying levels, two marked with asterisks. Graph B shows similar comparisons with slight variations. Both graphs range from zero to one hundred fifty percent hemolysis.

Figure 3. Hemolysis of erythrocytes co-incubated with monocultures or co-cultures of Trichomonas vaginalis, Candida albicans and Lactobacillus crispatus. (A) ATCC30236 T. vaginalis standard isolate, C. albicans (CA3), and L. crispatus (LC2). (B) TV-LACM6 T. vaginalis fresh clinical isolate, C. albicans (CA3), and L. crispatus (LC1). Positive control of hemolysis is erythrocytes treated with 0.2% Triton X-100. Results are expressed as a percentage of total hemolysis, presented as the mean ± S.D. of at least two blood samples. The percentage of hemolysis from erythrocytes co-incubated with T. vaginalis monocultures was compared to co-cultures with the protozoan. (*) indicates a significant difference.

Figure 5
Bar graphs A and B show the percentage of hyphae under different conditions. In graph A, the conditions are CA3, ATCC 30236 + CA3, CA3 + LC2, and ATCC 30236 + CA3 + LC2. In graph B, the conditions are CA3, TV-LACM6 + CA3, CA3 + LC1, and TV-LACM6 + CA3 + LC1. CA3 shows the highest percentage in both graphs, while combinations with LC2 or LC1 show reduced hyphae formation. Asterisks indicate statistical significance.

Figure 5. Yeast-to-hyphal form transition of Candida albicans in monoculture and co-culture. (A) Co-culture of C. albicans (CA3, initial density at 3.33x104 CFU/mL) with ATCC Trichomonas vaginalis isolate (ATCC3026, initial density at 1 x 106 trophozoites/mL) and second density of Lactobacillus crispatus (LC2, initial density at 5.53x106 CFU/mL). (B) Co-culture of CA3 with fresh clinical T. vaginalis isolate (TV-LACM6, initial density at 1 x 106 trophozoites/mL) and first density of L. crispatus (LC1, initial density at 5.53x107 CFU/mL. The date were expressed by percentage de hyphae formation. Results are representative of two independent experiments conducted with triplicate assays. (*) Statistically significant difference (p < 0.05).

The original article has been updated.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Keywords: Candida albicans, co-culture, Lactobacillus crispatus, Trichomonas vaginalis, vaginal microbiota, vaginitis

Citation: Cardoso FG, Santos LTd, Menezes SA, Rigo GV and Tasca T (2025) Correction: In vitro co-culture model of Trichomonas vaginalis, Candida albicans, and Lactobacillus crispatus: a system for assessing antimicrobial activity and microorganism interactions in vaginitis. Front. Parasitol. 4:1711595. doi: 10.3389/fpara.2025.1711595

Received: 23 September 2025; Accepted: 29 September 2025;
Published: 10 October 2025.

Approved by:

Frontiers Editorial Office, Frontiers Media SA, Switzerland

Copyright © 2025 Cardoso, Santos, Menezes, Rigo and Tasca. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Tiana Tasca, dGlhbmEudGFzY2FAdWZyZ3MuYnI=

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.