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Front. Pharmacol. | doi: 10.3389/fphar.2018.00165

Integrated metabolomics and network pharmacology approach to explain possible action mechanisms of Xin-Sheng-Hua Granule for treating anaemia

Han-Qing Pang1,  Shi-Jun Yue2,  Yu-Ping Tang1*, Yan-Yan Chen1, Ya-Jie Tan2, Yu-Jie Cao2, Xu-Qin Shi2, Gui-Sheng Zhou2,  An Kang2, Sheng-Liang Huang3, Ya-Jun Shi1, Jin Sun1, Zhi-Shu Tang1 and  Jin-ao Duan2
  • 1Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and College of Pharmacy, Shaanxi University of Chinese Medicine, China
  • 2Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, China
  • 3Jiangsu Revolence Pharmaceutical Co., Ltd., China

As a well-known traditional Chinese medicine (TCM) prescription, Xin-Sheng-Hua Granule (XSHG) has been applied in China for more than thirty years to treat postpartum diseases, especially anaemia. However, the underlying therapeutic mechanisms of XSHG for anaemia were still unclear. In this study, plasma metabolomics profiling for anaemia was firstly analyzed by UHPLC-QTOF/MS and multivariate data method. Using network pharmacology, the compound-target-pathway network of XSHG was constructed, so as to dissect anaemia-relevant signaling pathways. Finally, the crucial targets in the shared pathways of metabolomics and network pharmacology were experimentally validated by ELISA and Western Blot analysis. The results showed that XSHG could exert excellent effects on anaemia probably through regulating coenzyme A biosynthesis, sphingolipids metabolism, and HIF-1a signaling pathways, which was reflected by the increased levels of EPOR, F2, COASY, and the reduced protein expression of HIF-1a, SPHK1, and S1PR1. Our work successfully explained the polypharmcological mechanisms underlying the efficiency of XSHG on treating anaemia, and meanwhile, probed into the potential treatment strategies for anaemia from TCM prescription.

Keywords: Xin-Sheng-Hua granule, Postpartum Hemorrhage, Metabolomics, Network Pharmacology, molecular mechanism

Received: 16 Oct 2017; Accepted: 14 Feb 2018.

Edited by:

Muriel Cuendet, Université de Genève, Switzerland

Reviewed by:

William Chi-Shing Tai, Hong Kong Polytechnic University, Hong Kong
Pierre-Marie Allard, Université de Genève, Switzerland  

Copyright: © 2018 Pang, Yue, Tang, Chen, Tan, Cao, Shi, Zhou, Kang, Huang, Shi, Sun, Tang and Duan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Yu-Ping Tang, Shaanxi University of Chinese Medicine, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and College of Pharmacy, Shiji Road, Xianyang, 712046, Shaanxi Province, China, yupingtang9@126.com