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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Pharmacol. | doi: 10.3389/fphar.2018.01021

Protective effects of anti-IL17 on acute lung injury induced by LPS in mice

  • 1Faculdade de Medicina, Universidade de São Paulo, Brazil
  • 2Departamento de Ciências Biológicas, Federal University of São Paulo, Brazil
  • 3Department of Bioscience, Federal University of São Paulo, Brazil

Introduction: T helper 17 (Th17) has been implicated in a variety of inflammatory lung and immune system diseases. However, little is known about the expression and biological role of IL 17 in acute lung injury (ALI). We investigated the mechanisms involved in the effect of anti-IL17 in a model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Methods: Mice were pre-treated with anti-IL17, 1h before saline/LPS intratracheal administration alongside non-treated controls and levels of exhaled nitric oxide (eNO), cytokine expression, extracellular matrix remodeling and oxidative stress, as well as immune cell counts in bronchoalveolar lavage fluid (BALF), and respiratory mechanics were assessed in lung tissue. Results: LPS instillation led to an increase in multiple cytokines, proteases, nuclear factor-κB and Forkhead box P3 (FOXP3), eNO and regulators of the actomyosin cytoskeleton, the number of CD4+ and iNOS-positive cells as well as the number of neutrophils and macrophages in BALF, resistance and elastance of the respiratory system, ARG-1 gene expression, collagen fibers, and actin and 8-iso-PGF2α volume fractions. Pre-treatment with anti-IL17 led to a significant reduction in the level of all assessed factors. Conclusions: Anti-IL17 can protect the lungs from the inflammatory effects of LPS-induced ALI, primarily mediated by the reduced expression of cytokines and oxidative stress. This suggests that further studies using anti-IL17 in a treatment regime would be highly worthwhile.

Keywords: acute lung injury (ALI), Inflammation Mediators, IL-17, remodeling, Oxidative Stress

Received: 09 May 2018; Accepted: 23 Aug 2018.

Edited by:

Giuseppa Pistritto, Università degli Studi di Roma Tor Vergata, Italy

Reviewed by:

Nadia Mores, Università Cattolica del Sacro Cuore, Italy
Antonio Recchiuti, Università degli Studi G. d'Annunzio Chieti e Pescara, Italy  

Copyright: © 2018 Righetti, Santos, Camargo, Aristóteles, Fukuzaki, De Souza, Santana, Agrela, Cruz, Alonso-Vale, Saraiva-Romanholo, Leick, Martins, Prado and Tibério. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: PhD. Renato F. Righetti, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil, refragar@gmail.com